FastQCFastQC Report
Thu 26 May 2016
SRR1926867_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926867_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581399
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9540.16408696953383134No Hit
GTCCTAAAGTGTGTATTTCTCATTT7720.13278316612171676No Hit
CTGTAGGACGTGGAATATGGCAAGA7480.12865519204539397No Hit
CTTTAGGACGTGAAATATGGCGAGG6980.12005524605305479No Hit
GTATCAACGCAGAGTACTTTTTTTT6630.11403528385841738No Hit
GTCCTACAGTGTGCATTTCTCATTT6280.10801532166377995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAGG1700.012.82710219
TTGAAGT701.06207815E-412.2466234
TTAGACT550.003004355812.1229194
TAGGACC1900.012.031774
GTCTTAT657.698987E-411.7476321
GGACGTG3900.011.4710976
GTCCTAG751.9807297E-411.453941
TTAGGAC2750.011.4430393
TAGGACG3950.011.3337244
GTGTAGC1451.1168595E-911.1906321
AGGACGT4100.011.1436925
CTTTAGG2750.011.1068521
TAGACTC600.005783431711.1049995
GACGTGA2150.011.0671487
TAGTACA951.31683555E-511.0291224
AGTGAGC700.001477613710.8671028
TAGCCCT1855.456968E-1210.8123344
TGGCGAG4300.010.80381818
GTCCTAC5750.010.7899451
ACTGTCC803.7312414E-410.6973048
Produced by FastQC (version 0.11.5)