##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926867_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 581399 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.959690333144707 31.0 28.0 31.0 16.0 34.0 2 27.937330473564625 31.0 28.0 31.0 16.0 34.0 3 27.596963531069026 31.0 27.0 31.0 15.0 34.0 4 31.3517085512703 35.0 32.0 37.0 17.0 37.0 5 31.591475045536715 35.0 32.0 37.0 17.0 37.0 6 31.034695622111492 35.0 32.0 37.0 11.0 37.0 7 30.8143220060578 35.0 32.0 37.0 11.0 37.0 8 30.752017117332503 35.0 32.0 37.0 10.0 37.0 9 32.77295110586706 37.0 32.0 39.0 17.0 39.0 10 32.95095450800569 37.0 33.0 39.0 17.0 39.0 11 33.1281942349402 37.0 33.0 39.0 17.0 39.0 12 33.01964055665731 38.0 33.0 39.0 15.0 39.0 13 33.10578965564096 38.0 33.0 39.0 17.0 39.0 14 34.09597023730691 39.0 34.0 40.0 17.0 41.0 15 33.68150444015212 39.0 33.0 40.0 10.0 41.0 16 33.755720254076806 39.0 33.0 40.0 10.0 41.0 17 33.969313672710136 39.0 33.0 41.0 11.0 41.0 18 34.015003465778236 39.0 34.0 41.0 10.0 41.0 19 33.383416552144055 39.0 33.0 40.0 9.0 41.0 20 33.700272962285794 39.0 33.0 41.0 8.0 41.0 21 33.66553434044434 39.0 34.0 41.0 2.0 41.0 22 33.67991517013273 39.0 34.0 41.0 2.0 41.0 23 33.66247963962786 39.0 34.0 41.0 2.0 41.0 24 32.382952155060465 38.0 32.0 40.0 2.0 41.0 25 32.93197786717899 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.9496589593056655 1101 2 -1.2160060124957432 1101 3 -2.1733724337486784 1101 4 -1.6637310170762838 1101 5 -1.2503834384821424 1101 6 -3.26440729188138 1101 7 -2.103350792028813 1101 8 -1.147205544361448 1101 9 -1.3328891789779114 1101 10 -9.602706503367738 1101 11 -4.9683806927644945 1101 12 -2.7145647234972614 1101 13 -1.9995570340572577 1101 14 -2.091206262832003 1101 15 -3.3418983542428116 1101 16 -1.671085480024189 1101 17 -1.4935439728500413 1101 18 -1.3228225894509507 1101 19 -1.925081898412202 1101 20 -1.3201742488411767 1101 21 -1.097909052379876 1101 22 -1.1813500267719093 1101 23 -1.3543622173704222 1101 24 -1.3716638386563176 1101 25 -1.2573821885810084 1102 1 -0.35487045381692184 1102 2 -0.4307975152494805 1102 3 -0.69022268289633 1102 4 -0.6561254571497166 1102 5 -0.3872179519885677 1102 6 -0.8261977830581806 1102 7 -0.22315709377846815 1102 8 0.05859416340521406 1102 9 -0.1553574267018618 1102 10 -0.14231723357642068 1102 11 -0.22269862764891002 1102 12 -0.19987812501837254 1102 13 -0.5029008578180765 1102 14 -0.7184776277115503 1102 15 -0.13705402469352634 1102 16 -0.3070114475787946 1102 17 -0.38191458466675954 1102 18 -0.7714007618686196 1102 19 -0.3882717376838656 1102 20 -0.7014638221055733 1102 21 -0.9703192280945316 1102 22 -1.0543690219388182 1102 23 -1.149742682695809 1102 24 -4.321909239727841 1102 25 -2.721311977648483 1103 1 -0.26100386087528094 1103 2 -0.7528105336793836 1103 3 -0.16205199862239184 1103 4 -0.2633255731582125 1103 5 -0.34439238620998935 1103 6 0.3602438596546307 1103 7 -0.008115833514224846 1103 8 0.133529416841764 1103 9 -0.07094498397766813 1103 10 -0.10239865636098244 1103 11 -0.4308986393039689 1103 12 -0.5694410738567299 1103 13 -0.2831325224451078 1103 14 -0.45191423028894206 1103 15 -0.024832069001725188 1103 16 -0.18481357449154245 1103 17 -0.40382202994558014 1103 18 -0.7201361202006993 1103 19 -2.1222599227065757 1103 20 -0.9768159471887543 1103 21 -0.8644696081714329 1103 22 -0.518300002305466 1103 23 -0.49506039825003256 1103 24 1.1052866208288634 1103 25 0.3320695479987421 1104 1 -0.813186835494367 1104 2 -0.7568493571056365 1104 3 -2.104838031361954 1104 4 -1.3377780589771398 1104 5 -1.0955433969275745 1104 6 -2.3623601967054384 1104 7 -1.094013001346628 1104 8 -0.49772897208047695 1104 9 -0.5095094075280286 1104 10 -0.3957394540083712 1104 11 -0.5008172288184056 1104 12 -0.595582810388926 1104 13 -0.8882667114766107 1104 14 -0.8080092249267778 1104 15 -0.31991439004367095 1104 16 -0.674321941492984 1104 17 -0.7521172660494813 1104 18 -0.7713242276598464 1104 19 -1.8125169694012548 1104 20 -0.8899299825722977 1104 21 -0.6670974064460964 1104 22 -0.6016651489050417 1104 23 -0.553283396880822 1104 24 0.8259441099865015 1104 25 0.08244759751952557 1105 1 -0.1840737058494426 1105 2 -0.24236545950035193 1105 3 0.32263678284118313 1105 4 0.07164225481311703 1105 5 -0.04716684862038889 1105 6 -0.0030340199919791644 1105 7 -0.059747105392869315 1105 8 0.013716114624727993 1105 9 -0.6664375660746842 1105 10 -0.18944383055667657 1105 11 -0.22784613055251413 1105 12 -0.005016336400487376 1105 13 -0.09398099719089714 1105 14 -0.3996855255048146 1105 15 -0.022322317376456624 1105 16 -0.5207628993790223 1105 17 -0.32681586225096737 1105 18 -0.43799309636339245 1105 19 -1.1035888108545961 1105 20 -0.14041388017758294 1105 21 -6.002966195026715E-4 1105 22 0.29827209304375657 1105 23 -0.16142212520452404 1105 24 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0.4670218711424994 1107 11 0.026224354028983043 1107 12 0.30717749619863355 1107 13 -0.1367248612563472 1107 14 0.07449949652161791 1107 15 -1.2312688246137071 1107 16 -0.22909518383465866 1107 17 -0.06820038081610136 1107 18 -0.49603966653331355 1107 19 0.5955691501271474 1107 20 0.7123325011943464 1107 21 0.29743494567124173 1107 22 0.171037354214306 1107 23 -0.18744855692258255 1107 24 -3.4268161966010595 1107 25 -1.6263655215759947 1108 1 0.7008786751029383 1108 2 0.422617929203966 1108 3 0.8034164063184832 1108 4 0.8326787996636185 1108 5 0.9656437826088471 1108 6 -2.9703784275224336 1108 7 -1.0737094531226603 1108 8 0.4386552065405631 1108 9 0.2592767196396011 1108 10 0.7827023488453122 1108 11 0.7534783434231258 1108 12 0.8095484476526735 1108 13 0.628233842233243 1108 14 0.5205359584375984 1108 15 -0.5399657282291628 1108 16 0.4171330031680398 1108 17 0.4105927756227885 1108 18 0.47023281725786603 1108 19 0.398320380729011 1108 20 -1.02842273953528 1108 21 -0.295333408468899 1108 22 0.1656563676838445 1108 23 0.1308126477744338 1108 24 -0.6454108468369348 1108 25 -0.05005357071225802 1109 1 0.4752930895173506 1109 2 0.4540051968703054 1109 3 -0.8970080048036024 1109 4 0.5394723706432352 1109 5 0.7033505258969832 1109 6 0.6013829684250105 1109 7 1.0647934351476707 1109 8 1.2861485470571559 1109 9 0.5187361790990579 1109 10 0.9383708412579921 1109 11 0.5609106262042474 1109 12 0.263307987923838 1109 13 0.33984139663149193 1109 14 0.6878820420627534 1109 15 -0.9822296532837882 1109 16 0.34858072996460265 1109 17 0.28986367442857386 1109 18 0.16920381483351576 1109 19 -1.1510328262986178 1109 20 -0.24132507708878137 1109 21 0.31572402770481744 1109 22 0.5482489270205946 1109 23 0.5680913883089929 1109 24 -0.6470007423803175 1109 25 0.05818263891930542 1110 1 0.6605736642096893 1110 2 0.8284655395659399 1110 3 0.9536744423494312 1110 4 1.0448871972345302 1110 5 0.9809775647004919 1110 6 1.011360004284402 1110 7 1.2467365547378506 1110 8 1.6175505372826784 1110 9 1.1438083100535437 1110 10 1.1433628921323944 1110 11 0.7643281613864872 1110 12 0.8025130753642173 1110 13 0.7036722863447338 1110 14 0.5466761302686081 1110 15 1.3695009007997072 1110 16 0.9298502347635207 1110 17 0.5628448909392034 1110 18 0.6846922444395958 1110 19 -1.1198651880721542 1110 20 0.4626636174881469 1110 21 0.48865460534128147 1110 22 0.6587476610487357 1110 23 0.7289133867780464 1110 24 -0.37476615485161346 1110 25 0.14071576027595256 1111 1 0.3653737891094515 1111 2 -0.03288508102974674 1111 3 0.5240724893713526 1111 4 0.5659782250318237 1111 5 0.6078714637729696 1111 6 0.9623936207711665 1111 7 0.7992583234887789 1111 8 0.9247258866116965 1111 9 0.613239803244376 1111 10 0.3678311810766388 1111 11 0.08936559583185044 1111 12 0.3014592336320874 1111 13 0.06591352739418 1111 14 0.22324607058639145 1111 15 -0.8923344160269906 1111 16 -0.35571248833512925 1111 17 -0.0641944879683507 1111 18 -0.07433992434540215 1111 19 0.4482300196091842 1111 20 0.0506008553583257 1111 21 0.3437992690699616 1111 22 0.45467848361301577 1111 23 0.1413096880419502 1111 24 0.8555035860523503 1111 25 0.6128687908692996 1112 1 0.25537602960029204 1112 2 0.3123435352086439 1112 3 -1.2346542224498194 1112 4 -0.17064345947259696 1112 5 0.028938971485430898 1112 6 0.9014315884736313 1112 7 0.9714486618028957 1112 8 1.267472728381339 1112 9 0.19895925932213743 1112 10 0.4657096085967609 1112 11 0.42595281124643236 1112 12 0.5260736106894583 1112 13 0.3149078916979917 1112 14 0.5319546861353999 1112 15 1.0442596932055608 1112 16 0.8652688466527181 1112 17 0.15805186261675885 1112 18 0.6533508189805204 1112 19 1.3562630409972556 1112 20 0.8123056965419906 1112 21 0.9387012906820829 1112 22 0.9904625692342321 1112 23 0.8520381922557974 1112 24 2.024922609543033 1112 25 1.301219961956626 1113 1 -0.3834413823064651 1113 2 0.0518802732383854 1113 3 -4.506066277284912 1113 4 -0.967967771328663 1113 5 0.1080181429219742 1113 6 0.5771732689515865 1113 7 1.1073745307750542 1113 8 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0.3767051778180175 2215 3 0.7305274460551097 2215 4 1.0883120864920102 2215 5 1.0416341872017973 2215 6 1.7913690874745747 2215 7 1.249570115150938 2215 8 -0.07659607067235186 2215 9 0.7023784316234867 2215 10 0.3209886252050822 2215 11 0.5890884112400343 2215 12 0.4543109546367745 2215 13 0.5794070045354687 2215 14 0.740052641665379 2215 15 0.5630979099289704 2215 16 0.7928092485133647 2215 17 0.6015340154644733 2215 18 -0.2535914275387654 2215 19 0.7455851209537698 2215 20 -3.3477544067205294 2215 21 -1.4904935493888942 2215 22 -0.6863609094019338 2215 23 -0.36632846961539656 2215 24 -0.4694682854520913 2215 25 -0.25271472370011594 2216 1 -0.5610988988012409 2216 2 -0.9955735561946888 2216 3 -0.19100585586567576 2216 4 -0.5234904086498204 2216 5 -0.5524017401305095 2216 6 -1.6961413448791198 2216 7 -9.421639052385736 2216 8 -10.754438829310033 2216 9 -5.488939386374671 2216 10 -3.3465033907722024 2216 11 -2.491992919543339 2216 12 -1.9955321366043606 2216 13 -1.7029456105591834 2216 14 -1.7850881758615955 2216 15 -3.1525754118864278 2216 16 -2.065122881904145 2216 17 -2.1237694081953364 2216 18 -1.9345138477832293 2216 19 -1.4812011597697001 2216 20 -2.0365268844006827 2216 21 -5.308131387343256 2216 22 -3.449205408048421 2216 23 -3.3606310956428516 2216 24 -3.3832808757246724 2216 25 -2.7441856008801295 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37595.0 3 5.0 4 274.0 5 1607.0 6 2134.0 7 1677.0 8 1356.0 9 1187.0 10 1149.0 11 1101.0 12 1134.0 13 1057.0 14 1156.0 15 1171.0 16 1168.0 17 1350.0 18 1546.0 19 1931.0 20 2373.0 21 3148.0 22 3855.0 23 4687.0 24 5461.0 25 6237.0 26 7081.0 27 7794.0 28 8635.0 29 10139.0 30 12172.0 31 14943.0 32 19122.0 33 24840.0 34 33308.0 35 46531.0 36 67008.0 37 105374.0 38 140093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.68511927026147 18.942244545835322 12.688032294929098 25.684603888974117 2 14.368057588647599 20.223190452408424 42.052112215199685 23.356639743744296 3 18.53208512866083 24.814267558044616 29.402556339701796 27.251090973592756 4 11.579942030581966 17.548141467411135 38.93038850525649 31.94152799675041 5 12.521870177593582 38.03595913093892 36.791272917451586 12.650897774015906 6 31.50597735675074 37.53236408803376 18.708464294991888 12.253194260223612 7 28.018217732961848 31.28688616504645 22.84169031579129 17.853205786200412 8 24.23937173027731 38.033224608495715 21.135280491107387 16.592123170119585 9 26.11703179968863 15.692032444799972 20.54120541205412 37.649730343457286 10 14.298864260065692 29.66068887676852 35.153568350002665 20.886878513163122 11 33.82636279812284 23.022033221859818 24.726132970012248 18.425471010005094 12 22.86702219163943 25.20436951660072 32.260106657491825 19.668501634268022 13 28.571158246030514 22.54459754691989 25.718635495690766 23.16560871135883 14 20.327366872812036 22.64766344689965 28.133079880613447 28.891889799674868 15 22.850247113851278 31.358085431034333 23.351011764786836 22.440655690327553 16 21.490539194519805 28.537587756948735 28.563564329864626 21.408308718666834 17 21.399768792491262 28.95822438138229 28.098421458258787 21.543585367867664 18 21.20410382032368 28.221849884399425 29.82185126059672 20.75219503468017 19 23.319163284721636 27.370959944810785 27.424635240239958 21.88524153022762 20 23.062194866007687 27.914922019879512 28.273771636773997 20.749111477338804 21 22.61450119042704 27.334234754964697 27.205730564386275 22.845533490221985 22 22.46984770316137 28.69831188424546 27.460222708107185 21.37161770448599 23 22.009767745109677 28.325944734311488 27.720750523394937 21.9435369971839 24 22.83808216397357 27.56128064462301 28.322504175977674 21.278133015425745 25 22.456102927388987 27.676616153709855 28.172570931166707 21.694709987734452 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 175.0 1 175.0 2 234.5 3 294.0 4 294.0 5 294.0 6 438.0 7 582.0 8 582.0 9 582.0 10 880.5 11 1179.0 12 1179.0 13 1179.0 14 2012.5 15 2846.0 16 2846.0 17 2846.0 18 4991.0 19 7136.0 20 7136.0 21 7136.0 22 11245.5 23 15355.0 24 15355.0 25 15355.0 26 21976.0 27 28597.0 28 28597.0 29 28597.0 30 37268.5 31 45940.0 32 45940.0 33 45940.0 34 52383.0 35 58826.0 36 58826.0 37 58826.0 38 63774.0 39 68722.0 40 68722.0 41 68722.0 42 71572.0 43 74422.0 44 74422.0 45 74422.0 46 75559.5 47 76697.0 48 76697.0 49 76697.0 50 73023.5 51 69350.0 52 69350.0 53 69350.0 54 60932.0 55 52514.0 56 52514.0 57 52514.0 58 44373.0 59 36232.0 60 36232.0 61 36232.0 62 29206.5 63 22181.0 64 22181.0 65 22181.0 66 16975.0 67 11769.0 68 11769.0 69 11769.0 70 8566.0 71 5363.0 72 5363.0 73 5363.0 74 3727.0 75 2091.0 76 2091.0 77 2091.0 78 1433.5 79 776.0 80 776.0 81 776.0 82 508.0 83 240.0 84 240.0 85 240.0 86 159.0 87 78.0 88 78.0 89 78.0 90 50.5 91 23.0 92 23.0 93 23.0 94 16.0 95 9.0 96 9.0 97 9.0 98 5.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21431065412909206 2 0.12538721256830507 3 0.12469921688891793 4 0.0693155646982537 5 0.022015861740388273 6 0.02098386822130757 7 0.2999661162127902 8 0.28345421990749897 9 0.01788788766406547 10 0.03302379261058241 11 0.01633989738544442 12 0.017027893064831552 13 0.015307903866363718 14 0.016167898465597637 15 0.015135904946516935 16 0.018231885503759038 17 0.017715888744218686 18 0.014963906026670153 19 0.021671863900694706 20 0.01668389522513799 21 0.01633989738544442 22 0.017199891984678334 23 0.01685589414498477 24 0.01685589414498477 25 0.016511896305291204 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 581399.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.8835255463971 #Duplication Level Percentage of deduplicated Percentage of total 1 94.31174597500386 82.88448736719565 2 4.345357201258792 7.637706212100953 3 0.6763637268410431 1.7832368659947344 4 0.22940302148469596 0.8064298519628383 5 0.1181402496856877 0.5191290825654934 6 0.07141091162778235 0.37655056057990294 7 0.0405014429076024 0.24915867147053536 8 0.03729083427207898 0.26217999891173704 9 0.023089223578088375 0.18262461331542634 >10 0.12359598114252802 2.0558026405553487 >50 0.010767643668726148 0.6608598640151768 >100 0.010963367581552242 1.727072665340757 >500 0.001370420947565388 0.8547616059915037 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 954 0.16408696953383134 No Hit GTCCTAAAGTGTGTATTTCTCATTT 772 0.13278316612171676 No Hit CTGTAGGACGTGGAATATGGCAAGA 748 0.12865519204539397 No Hit CTTTAGGACGTGAAATATGGCGAGG 698 0.12005524605305479 No Hit GTATCAACGCAGAGTACTTTTTTTT 663 0.11403528385841738 No Hit GTCCTACAGTGTGCATTTCTCATTT 628 0.10801532166377995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCGAGG 170 0.0 12.827102 19 TTGAAGT 70 1.06207815E-4 12.246623 4 TTAGACT 55 0.0030043558 12.122919 4 TAGGACC 190 0.0 12.03177 4 GTCTTAT 65 7.698987E-4 11.747632 1 GGACGTG 390 0.0 11.471097 6 GTCCTAG 75 1.9807297E-4 11.45394 1 TTAGGAC 275 0.0 11.443039 3 TAGGACG 395 0.0 11.333724 4 GTGTAGC 145 1.1168595E-9 11.190632 1 AGGACGT 410 0.0 11.143692 5 CTTTAGG 275 0.0 11.106852 1 TAGACTC 60 0.0057834317 11.104999 5 GACGTGA 215 0.0 11.067148 7 TAGTACA 95 1.31683555E-5 11.029122 4 AGTGAGC 70 0.0014776137 10.867102 8 TAGCCCT 185 5.456968E-12 10.812334 4 TGGCGAG 430 0.0 10.803818 18 GTCCTAC 575 0.0 10.789945 1 ACTGTCC 80 3.7312414E-4 10.697304 8 >>END_MODULE