FastQCFastQC Report
Thu 26 May 2016
SRR1926867_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926867_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences581399
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12310.2117306703313903No Hit
CTGTAGGACGTGGAATATGGCAAGA9400.16167898465597635No Hit
GTCCTACAGTGGACATTTCTAAATT9080.15617501922087929No Hit
CTTTAGGACGTGAAATATGGCGAGG8090.13914712615604774No Hit
GTCCTAAAGTGTGTATTTCTCATTT7750.1332991628812571No Hit
GGTATCAACGCAGAGTACTTTTTTT7090.1219472341713694No Hit
GTCCTACAGTGTGCATTTCTCATTT6940.11936725037366765No Hit
TATCAACGCAGAGTACTTTTTTTTT6360.10939131302255421No Hit
CTGTAGGACCTGGAATATGGCGAGA6170.10612333354546534No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTAT350.002115039916.3461021
CTAGCAC456.6312274E-414.8133663
GTGTAGA1101.7462298E-1013.8694211
TAGCACA903.5106495E-813.7552684
CCTGGAC655.3195563E-513.1855253
GTATTAC550.002980656512.1357431
CTGGACC657.850074E-411.7204674
TAGGACT1251.7576895E-811.4274544
AATCCCG752.0947005E-411.38518819
TTAGCAC600.00576398611.1100253
TTAGGAC4150.011.0144133
GTATTAA700.001443675310.8974021
TAGGCAG803.6802594E-410.7132395
GGACGTG6200.010.5980426
GTATAAA909.140924E-510.5946961
CACTGTT1909.094947E-1210.4881627
TTTAGGA4300.010.4222242
AATGTCC1659.404175E-1010.3510628
TACACTG3500.010.3391255
AGGACGT6200.010.2908525
Produced by FastQC (version 0.11.5)