##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926867_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 581399 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.830438304847444 31.0 30.0 33.0 26.0 34.0 2 30.1571605730316 31.0 30.0 34.0 26.0 34.0 3 30.20983524223468 31.0 30.0 34.0 26.0 34.0 4 33.80513898372718 35.0 35.0 37.0 28.0 37.0 5 33.74855477907599 37.0 35.0 37.0 28.0 37.0 6 33.66118964772901 37.0 35.0 37.0 29.0 37.0 7 33.655412204011355 37.0 35.0 37.0 28.0 37.0 8 33.68550341503855 37.0 35.0 37.0 29.0 37.0 9 35.22685453535352 39.0 35.0 39.0 29.0 39.0 10 35.13514127131282 39.0 35.0 39.0 29.0 39.0 11 35.15003637777155 39.0 35.0 39.0 29.0 39.0 12 35.05987626397706 39.0 35.0 39.0 28.0 39.0 13 35.06790517355551 39.0 35.0 39.0 29.0 39.0 14 36.16342305370322 40.0 36.0 41.0 29.0 41.0 15 36.137919053868345 40.0 36.0 41.0 28.0 41.0 16 36.11829225712462 40.0 36.0 41.0 28.0 41.0 17 36.09159286479681 40.0 36.0 41.0 28.0 41.0 18 36.073801296527854 40.0 36.0 41.0 28.0 41.0 19 36.138462570455054 40.0 37.0 41.0 28.0 41.0 20 36.09547832039615 40.0 37.0 41.0 28.0 41.0 21 36.05184391442022 40.0 36.0 41.0 28.0 41.0 22 35.957695145674485 40.0 36.0 41.0 27.0 41.0 23 35.89017524969943 40.0 36.0 41.0 27.0 41.0 24 35.819610972843094 40.0 36.0 41.0 27.0 41.0 25 35.69326744628044 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.408732887590908 1101 2 -0.22698907735575347 1101 3 -0.04642009162121141 1101 4 -0.16162482376567766 1101 5 -0.36131251763899996 1101 6 -0.5985976145512311 1101 7 -0.6908561050118962 1101 8 -0.8459253902315496 1101 9 -0.6534482059122269 1101 10 -0.6906115367498131 1101 11 -0.766892942101677 1101 12 -0.5541236674619654 1101 13 -0.5723655616579322 1101 14 -0.8319123981150867 1101 15 -0.7248246957357978 1101 16 -0.4374060408805107 1101 17 -0.602656226454414 1101 18 -0.7726806720420072 1101 19 -0.7948408865345158 1101 20 -1.0349002094687734 1101 21 -0.7672106269598373 1101 22 -0.9772789185392199 1101 23 -0.7484070616532463 1101 24 -0.7513867173869926 1101 25 -0.638158897438025 1102 1 -0.43770332706488446 1102 2 -0.4355050565247609 1102 3 -0.34471352386428933 1102 4 -0.5640919475152586 1102 5 -0.4649054890525903 1102 6 -0.4484440047202014 1102 7 -0.2894380240108134 1102 8 -0.5163386381428268 1102 9 -0.4503835024541303 1102 10 -0.2988221695473854 1102 11 -0.5568876968879408 1102 12 -0.3445211797320198 1102 13 -0.38477423874009276 1102 14 -0.44121890592670354 1102 15 -0.6111253214148675 1102 16 -0.49802572541837264 1102 17 -0.6519331362742022 1102 18 -0.4542961978746831 1102 19 -0.6515903526466857 1102 20 -0.6904114304795996 1102 21 -0.7449278350074948 1102 22 -0.6893166840781646 1102 23 -0.6505744589032574 1102 24 -0.8157436167163539 1102 25 -0.7255760043279835 1103 1 -0.6715065219063021 1103 2 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10 0.4569300292584302 1107 11 0.4731038097609215 1107 12 0.5066520283499685 1107 13 0.6015417446211231 1107 14 0.7103336888943943 1107 15 0.575237221883313 1107 16 0.5664866487802627 1107 17 0.4034949337396654 1107 18 0.4683260475422344 1107 19 0.5494718278933064 1107 20 0.4293550535288517 1107 21 0.5538373033676152 1107 22 0.3744486845383932 1107 23 0.4431670669323893 1107 24 0.251516305410874 1107 25 0.5565618642501988 1108 1 0.3550741355934619 1108 2 0.26765244016648637 1108 3 0.29040518612679733 1108 4 0.34293881714454955 1108 5 0.1713206406505705 1108 6 0.2846678446707216 1108 7 0.24147519209809332 1108 8 0.08666490019820117 1108 9 0.2322732244453647 1108 10 0.2787647358897729 1108 11 0.21198570613180578 1108 12 0.0866972520536251 1108 13 -0.04476937689745597 1108 14 -0.09540684315491887 1108 15 -0.08194076046626719 1108 16 0.19378747178336653 1108 17 0.17711907451368347 1108 18 0.023947056318654347 1108 19 0.027203910379000718 1108 20 0.028195921706881677 1108 21 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0.4541755757102095 2214 12 0.34432751494237124 2214 13 0.20027721232036555 2214 14 0.3111502101211414 2214 15 0.11317319090601785 2214 16 0.26449304626616055 2214 17 0.465531373220756 2214 18 0.2894139007367684 2214 19 0.418828431613953 2214 20 0.4434500992185022 2214 21 0.22630987554978077 2214 22 0.32280815964524834 2214 23 0.2929477379201657 2214 24 0.1231106262391819 2214 25 0.26337181963614853 2215 1 0.6813179753622087 2215 2 0.6748282522463924 2215 3 0.4270126008203512 2215 4 0.4799410219469351 2215 5 0.6556913017232588 2215 6 0.8021674180966585 2215 7 0.541539094564861 2215 8 0.39968327132098125 2215 9 0.35103478794802356 2215 10 0.7540251133492646 2215 11 0.6135106665691552 2215 12 0.5496789879925785 2215 13 0.537616292890732 2215 14 0.6568824376244962 2215 15 0.857552636836445 2215 16 0.6574283064633377 2215 17 0.8597847776248315 2215 18 0.778156756780028 2215 19 0.6273517893858696 2215 20 0.6521081578582795 2215 21 0.4553205938902636 2215 22 0.7771541869317957 2215 23 0.8195351235046076 2215 24 0.4464857168415506 2215 25 0.43827127505978325 2216 1 -0.3581109321894296 2216 2 -0.14075853107785363 2216 3 -2.0626043200723814 2216 4 -1.3688137168823644 2216 5 -0.7744665925273182 2216 6 -0.6328039872453175 2216 7 -0.45628050501753137 2216 8 -0.31607012169619253 2216 9 -0.5015294922888671 2216 10 -0.43349240222612195 2216 11 -0.6388953190611417 2216 12 -0.32270599434556146 2216 13 -0.4080030424567056 2216 14 -0.6122738274850974 2216 15 -0.5655343543649636 2216 16 -0.44820100698770204 2216 17 -0.6165926780203392 2216 18 -0.7159718683666441 2216 19 -0.6712460406845651 2216 20 -0.66055140710872 2216 21 -0.8459873383941385 2216 22 -0.8161764978925561 2216 23 -0.884918991077285 2216 24 -0.9985869785706782 2216 25 -1.0936601963101396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14583.0 3 14.0 4 262.0 5 1679.0 6 2189.0 7 1566.0 8 1246.0 9 1014.0 10 953.0 11 909.0 12 807.0 13 749.0 14 708.0 15 767.0 16 795.0 17 830.0 18 870.0 19 1029.0 20 1421.0 21 1760.0 22 2302.0 23 2767.0 24 3238.0 25 4022.0 26 4674.0 27 5183.0 28 6030.0 29 7286.0 30 9198.0 31 11869.0 32 15362.0 33 20435.0 34 27915.0 35 38616.0 36 56998.0 37 98535.0 38 232818.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.32774923927625 19.686686860789706 10.61672438466188 28.368839515272164 2 13.902591465776846 21.329359442553343 40.24494181211486 24.52310727955495 3 18.02217462734068 25.311932839608115 29.640199898502484 27.025692634548726 4 11.884781363573037 17.796040240867285 38.50092621418337 31.818252181376298 5 12.71318362818539 38.824490638411326 35.504373328287734 12.957952405115547 6 31.097651489783306 38.34582442961546 18.217265675434025 12.339258405167206 7 27.622054016858765 32.04300705315672 22.12145191811457 18.213487011869947 8 24.6815149669904 38.116371317597434 20.23528157069972 16.966832144712445 9 26.09066607951946 15.854350795228969 20.112773548100773 37.9422095771508 10 14.451158366573269 29.99334341281677 34.36123423790677 21.194263982703195 11 33.727056468050634 23.091199625713614 24.626014624002146 18.55572928223361 12 22.995739586755395 25.241701482114692 31.937447432830123 19.82511149829979 13 28.670647888874363 22.4189708907526 25.784835120984667 23.12554609938837 14 20.497146533011808 22.767020182387967 27.817949150151875 28.917884134448347 15 22.518202286616567 31.469616414652645 23.396280937007102 22.615900361723686 16 21.61775860556556 28.794094224772405 28.102484188944903 21.485662980717134 17 21.22191472637552 29.21883250573186 27.905620752701672 21.653632015190944 18 21.006852472325228 28.19678841959697 29.842138576804793 20.954220531273005 19 22.924571463954123 27.40669902545244 27.702021678781147 21.966707831812286 20 22.99826624194181 27.99193664903095 28.218976394746438 20.790820714280798 21 22.405233570263743 27.239026773365627 27.322801054145362 23.032938602225265 22 22.44310705728768 28.637304157729886 27.44586763995122 21.47372114503121 23 21.83629486806823 28.260454524345587 27.73998579288922 22.163264814696966 24 22.533578489127088 27.432451724203172 28.550960699966804 21.483009086702936 25 22.287699743708824 27.735521268727314 28.113077730189033 21.86370125737482 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 162.0 1 162.0 2 168.5 3 175.0 4 175.0 5 175.0 6 352.0 7 529.0 8 529.0 9 529.0 10 790.0 11 1051.0 12 1051.0 13 1051.0 14 1834.0 15 2617.0 16 2617.0 17 2617.0 18 4709.0 19 6801.0 20 6801.0 21 6801.0 22 10952.0 23 15103.0 24 15103.0 25 15103.0 26 21566.0 27 28029.0 28 28029.0 29 28029.0 30 36723.0 31 45417.0 32 45417.0 33 45417.0 34 51998.5 35 58580.0 36 58580.0 37 58580.0 38 63318.0 39 68056.0 40 68056.0 41 68056.0 42 70998.0 43 73940.0 44 73940.0 45 73940.0 46 75790.0 47 77640.0 48 77640.0 49 77640.0 50 73937.5 51 70235.0 52 70235.0 53 70235.0 54 61644.5 55 53054.0 56 53054.0 57 53054.0 58 45097.0 59 37140.0 60 37140.0 61 37140.0 62 29808.0 63 22476.0 64 22476.0 65 22476.0 66 17238.5 67 12001.0 68 12001.0 69 12001.0 70 8677.0 71 5353.0 72 5353.0 73 5353.0 74 3691.0 75 2029.0 76 2029.0 77 2029.0 78 1382.0 79 735.0 80 735.0 81 735.0 82 467.5 83 200.0 84 200.0 85 200.0 86 129.5 87 59.0 88 59.0 89 59.0 90 36.5 91 14.0 92 14.0 93 14.0 94 8.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12074324173244191 2 0.1045753432668443 3 0.01788788766406547 4 0.0 5 0.005847963274790634 6 0.3531137824454462 7 0.017027893064831552 8 0.009115942751879519 9 0.009803938431266652 10 0.0034399783969356673 11 0.004471971916016368 12 0.0 13 5.159967595403501E-4 14 1.7199891984678338E-4 15 0.002751982717548534 16 3.4399783969356676E-4 17 0.0 18 5.159967595403501E-4 19 1.7199891984678338E-4 20 5.159967595403501E-4 21 0.012899918988508752 22 0.0 23 0.0 24 0.0 25 0.004987968675556718 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 581399.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.35468110673946 #Duplication Level Percentage of deduplicated Percentage of total 1 92.8025651733863 79.21133356261792 2 5.560634292389581 9.492523335562252 3 0.8340422593501002 2.135682332291169 4 0.284286487504261 0.9706072993552509 5 0.13491260512712172 0.5757711193952469 6 0.08285420851751268 0.4243196727818155 7 0.06012092184236317 0.3592121478188681 8 0.04064410622879449 0.27753317808217576 9 0.026983190020121412 0.20728274214690162 >10 0.1461469689938542 2.3451029905543987 >50 0.01269941075729929 0.7439701617935128 >100 0.012296184697921812 1.9973892044471822 >500 0.0016126143865126577 1.0474722608549436 >1k 2.015767983140822E-4 0.21179999229836327 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1231 0.2117306703313903 No Hit CTGTAGGACGTGGAATATGGCAAGA 940 0.16167898465597635 No Hit GTCCTACAGTGGACATTTCTAAATT 908 0.15617501922087929 No Hit CTTTAGGACGTGAAATATGGCGAGG 809 0.13914712615604774 No Hit GTCCTAAAGTGTGTATTTCTCATTT 775 0.1332991628812571 No Hit GGTATCAACGCAGAGTACTTTTTTT 709 0.1219472341713694 No Hit GTCCTACAGTGTGCATTTCTCATTT 694 0.11936725037366765 No Hit TATCAACGCAGAGTACTTTTTTTTT 636 0.10939131302255421 No Hit CTGTAGGACCTGGAATATGGCGAGA 617 0.10612333354546534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCCCTAT 35 0.0021150399 16.346102 1 CTAGCAC 45 6.6312274E-4 14.813366 3 GTGTAGA 110 1.7462298E-10 13.869421 1 TAGCACA 90 3.5106495E-8 13.755268 4 CCTGGAC 65 5.3195563E-5 13.185525 3 GTATTAC 55 0.0029806565 12.135743 1 CTGGACC 65 7.850074E-4 11.720467 4 TAGGACT 125 1.7576895E-8 11.427454 4 AATCCCG 75 2.0947005E-4 11.385188 19 TTAGCAC 60 0.005763986 11.110025 3 TTAGGAC 415 0.0 11.014413 3 GTATTAA 70 0.0014436753 10.897402 1 TAGGCAG 80 3.6802594E-4 10.713239 5 GGACGTG 620 0.0 10.598042 6 GTATAAA 90 9.140924E-5 10.594696 1 CACTGTT 190 9.094947E-12 10.488162 7 TTTAGGA 430 0.0 10.422224 2 AATGTCC 165 9.404175E-10 10.351062 8 TACACTG 350 0.0 10.339125 5 AGGACGT 620 0.0 10.290852 5 >>END_MODULE