FastQCFastQC Report
Thu 26 May 2016
SRR1926866_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926866_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT15670.25204474719123715No Hit
CTGTAGGACGTGGAATATGGCAAGA12710.20443450777285413No Hit
GTCCTAAAGTGTGTATTTCTCATTT12510.20121759970404446No Hit
GTCCTACAGTGTGCATTTCTCATTT11640.18722404960472241No Hit
CTTTAGGACGTGAAATATGGCGAGG10850.17451726273292426No Hit
GTATCAACGCAGAGTACTTTTTTTT7610.12240335201820769No Hit
CTGTAGGACCTGGAATATGGCGAGA7590.12208166121132674No Hit
GTACATGGGAAGCAGTGGTATCAAC6350.10213683118470683No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACT654.7111826E-1019.0452354
CCTATAC508.426376E-515.2571383
ATCCCGT755.963011E-815.17123710
CCAACGA953.110472E-1014.97282119
TCCTATA1151.8189894E-1214.9254622
TCCAACG1300.014.58890218
TAGGACC3550.014.4851074
CCCGTTT903.7160135E-813.69625712
GTATAAG852.5523332E-713.4698081
TTGTAGT500.001471170213.3316644
GATATAC2150.013.3131811
GTCTTAA655.2260155E-513.21077251
CCGTTTC957.5353455E-812.97644513
TAAGACT1051.9197614E-812.6968224
AGGACCT4850.012.5578135
TGTAGGA9000.012.3964252
CTATCCC701.0649524E-412.2433644
GGACCTG4750.012.2211486
GTAGGAC8900.012.107143
CGTTTCC1103.8940925E-812.06900214
Produced by FastQC (version 0.11.5)