FastQCFastQC Report
Thu 26 May 2016
SRR1926866_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926866_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences621715
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16060.258317717925416No Hit
CTGTAGGACGTGGAATATGGCAAGA14860.239016269512558No Hit
CTTTAGGACGTGAAATATGGCGAGG13250.2131201595586402No Hit
GTCCTAAAGTGTGTATTTCTCATTT13150.21151170552423537No Hit
GTATCAACGCAGAGTACTTTTTTTT12550.20186098131780636No Hit
GTCCTACAGTGTGCATTTCTCATTT11750.18899334904256773No Hit
CTGTAGGACCTGGAATATGGCGAGA9110.1465301625342802No Hit
GGTATCAACGCAGAGTACTTTTTTT8350.13430591187280344No Hit
GTACATGGGAAGCAGTGGTATCAAC7470.12015151637004093No Hit
TATCAACGCAGAGTACTTTTTTTTT7420.11934728935283853No Hit
CTGAAGGACCTGGAATATGGCGAGA6490.10438866683287358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG602.5998756E-514.22864718
CCAACGA400.005327119514.22864719
GGTAGGA655.2583793E-513.2020892
ATAGGAC1051.9028448E-812.7049063
GTAGGAG603.9832536E-412.7049063
AAAGGCG603.9859704E-412.703885
TAGGACC3900.012.215274
CCTCTAG550.00298195912.1352541
AAGGCGT550.002998253612.12643056
TAGGCTG657.816692E-411.7266595
TGTAGGA9900.011.55738452
CTGTAGG9750.011.4418121
GTAGGAC10000.011.2438423
GGACCTG5350.011.2197826
CCTTATA951.30247645E-511.0403442
GTCCTAG951.30247645E-511.0403441
GATATAC1900.011.0403441
AGGACCT5550.010.987145
ACTGTTC2350.010.8994158
GTGTTAG700.001444406510.8969631