FastQCFastQC Report
Thu 26 May 2016
SRR1926865_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926865_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699548
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17100.24444355498121645No Hit
CTGTAGGACGTGGAATATGGCAAGA13590.19426829895875625No Hit
GTCCTACAGTGTGCATTTCTCATTT12540.1792586069862254No Hit
GTCCTAAAGTGTGTATTTCTCATTT12310.17597076969700434No Hit
CTTTAGGACGTGAAATATGGCGAGG10270.1468090824360873No Hit
CTGTAGGACCTGGAATATGGCGAGA8470.12107818191174874No Hit
GTATCAACGCAGAGTACTTTTTTTT8150.11650379959631076No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAAGTC350.002116468516.3451213
TCCAACG601.4963043E-615.80021218
CGGTTTC508.859852E-515.16820313
AATCCCG704.5320303E-714.89734419
TAGGACC3850.013.3646344
GTATTAG500.001454389313.3523561
GTAGGAC8200.012.7903893
TGTAGGA8750.012.7491942
AAATCCC905.529346E-712.64016918
CTGTAGG8150.012.6385131
GACGTGG3900.012.450497
CTAGACT853.8049511E-612.3309854
AGGACGT6950.012.1931885
GGACGTG6800.012.1821076
TATACTG1501.2732926E-1112.0607535
GTCCTAG959.889573E-712.0472371
TAGGACG7150.011.9939014
GGCGAGG3250.011.95954619
TCTAGGA1459.094947E-1111.8361222
AGGACCT5400.011.8138965
Produced by FastQC (version 0.11.5)