##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926865_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 699548 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.01080983720917 31.0 28.0 31.0 16.0 34.0 2 28.010749798441278 31.0 28.0 31.0 16.0 34.0 3 27.681238456832126 31.0 28.0 31.0 16.0 34.0 4 31.428603898517327 35.0 32.0 37.0 17.0 37.0 5 31.651519266726513 35.0 32.0 37.0 17.0 37.0 6 31.11256840131056 35.0 32.0 37.0 11.0 37.0 7 30.903775009005816 35.0 32.0 37.0 11.0 37.0 8 30.810800688444537 35.0 32.0 37.0 10.0 37.0 9 32.8303904807104 37.0 32.0 39.0 17.0 39.0 10 32.991271506744354 37.0 33.0 39.0 17.0 39.0 11 33.172935952929606 38.0 33.0 39.0 17.0 39.0 12 33.04499190906128 38.0 33.0 39.0 16.0 39.0 13 33.14828432073281 38.0 33.0 39.0 17.0 39.0 14 34.12858302789802 39.0 34.0 41.0 17.0 41.0 15 33.73247296825951 39.0 33.0 41.0 10.0 41.0 16 33.80312859160487 39.0 33.0 41.0 11.0 41.0 17 34.027709320875765 39.0 34.0 41.0 13.0 41.0 18 34.07362468336697 39.0 34.0 41.0 11.0 41.0 19 33.45003202067621 39.0 33.0 41.0 10.0 41.0 20 33.74475518477646 39.0 33.0 41.0 10.0 41.0 21 33.70768982257115 39.0 34.0 41.0 2.0 41.0 22 33.73342644107338 39.0 34.0 41.0 2.0 41.0 23 33.72351718538256 39.0 34.0 41.0 2.0 41.0 24 32.44053160040483 38.0 32.0 40.0 2.0 41.0 25 32.998057317010414 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.36333418711607735 1101 2 -0.4773233752116717 1101 3 -1.4682713946016577 1101 4 -0.6245142752305739 1101 5 -0.39673224418551456 1101 6 -2.4869548213006496 1101 7 -1.0341892224800837 1101 8 -0.5926753871502974 1101 9 -0.6750483174297628 1101 10 -9.156467034459702 1101 11 -4.309094081212667 1101 12 -1.9892990926895955 1101 13 -1.3494413998295585 1101 14 -1.1749352924325862 1101 15 -2.5261097766644376 1101 16 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2216 15 -2.9094513519769016 2216 16 -1.7267394253732107 2216 17 -1.9974903802226578 2216 18 -1.8150605854237938 2216 19 -1.0437410359234534 2216 20 -2.1494045664405377 2216 21 -4.549759539192166 2216 22 -3.4519748071609015 2216 23 -3.330274951057561 2216 24 -3.3437943732860056 2216 25 -2.812924945297187 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44593.0 3 0.0 4 325.0 5 1921.0 6 2506.0 7 2050.0 8 1622.0 9 1492.0 10 1368.0 11 1309.0 12 1284.0 13 1350.0 14 1373.0 15 1392.0 16 1464.0 17 1634.0 18 1841.0 19 2397.0 20 2953.0 21 3788.0 22 4710.0 23 5507.0 24 6582.0 25 7316.0 26 8499.0 27 9180.0 28 10475.0 29 12302.0 30 14679.0 31 17493.0 32 22668.0 33 29865.0 34 39561.0 35 55092.0 36 79388.0 37 125845.0 38 173724.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08647953263289 18.62061950782049 12.594873058486789 25.698027901059827 2 14.296244874006112 19.67708922270967 42.54535565272778 23.481310250556433 3 19.179823368592817 24.077122368063208 28.99030960594306 27.752744657400914 4 11.618113626415754 17.079007521120072 38.39747789294205 32.90540095952213 5 12.554795245216766 37.86412208938238 36.746199004320715 12.834883661080148 6 32.2161315135554 37.09670501239588 18.25139471509495 12.43576875895377 7 28.54334631890009 30.859527902718654 22.275719379847185 18.32140639853407 8 24.36115989820424 38.64855371160257 20.503387218179864 16.486899172013334 9 26.109956222245735 15.66734625601551 20.29203279185408 37.930664729884676 10 14.635816989725514 29.47612272184526 34.72547743831376 21.16258285011547 11 34.2582849626855 22.601292425585452 24.596974809138477 18.54344780259057 12 23.35503130312202 24.778433371315504 32.06915137729448 19.797383948268 13 29.035869150300446 22.295231028552067 25.398200609328065 23.27069921181942 14 20.222632386349826 22.80380699665022 27.517088451052185 29.456472165947766 15 22.841457399583966 31.973007941784076 22.933098868420863 22.252435790211088 16 21.505806796589837 28.333993386128025 28.779636251338577 21.380563565943564 17 21.305831606969505 29.183483523412068 28.17211311221579 21.338571757402633 18 21.16354309781591 27.957859341470005 30.208001235226252 20.670596325487836 19 23.71067986428736 27.061603988453275 27.123941442826116 22.103774704433253 20 23.318574138860335 27.74733326566121 28.303769670784657 20.630322924693793 21 22.564928102295177 27.118544718399463 27.0014553959735 23.315071783331856 22 22.761611199592828 28.787025308311915 27.23795989443194 21.21340359766332 23 21.685467152762886 28.447351490456786 27.93266137679605 21.934519979984273 24 23.278429309341593 27.338686964142493 28.200919848512356 21.181963878003554 25 22.41570343047901 27.70231534101063 28.1216358216637 21.760345406846664 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 194.0 2 256.0 3 318.0 4 318.0 5 318.0 6 487.5 7 657.0 8 657.0 9 657.0 10 963.5 11 1270.0 12 1270.0 13 1270.0 14 2222.5 15 3175.0 16 3175.0 17 3175.0 18 5487.5 19 7800.0 20 7800.0 21 7800.0 22 12493.0 23 17186.0 24 17186.0 25 17186.0 26 25033.0 27 32880.0 28 32880.0 29 32880.0 30 43597.5 31 54315.0 32 54315.0 33 54315.0 34 62100.5 35 69886.0 36 69886.0 37 69886.0 38 75509.5 39 81133.0 40 81133.0 41 81133.0 42 85141.5 43 89150.0 44 89150.0 45 89150.0 46 91070.5 47 92991.0 48 92991.0 49 92991.0 50 88839.5 51 84688.0 52 84688.0 53 84688.0 54 74612.5 55 64537.0 56 64537.0 57 64537.0 58 54705.0 59 44873.0 60 44873.0 61 44873.0 62 36442.0 63 28011.0 64 28011.0 65 28011.0 66 21629.0 67 15247.0 68 15247.0 69 15247.0 70 11129.5 71 7012.0 72 7012.0 73 7012.0 74 4874.5 75 2737.0 76 2737.0 77 2737.0 78 1883.5 79 1030.0 80 1030.0 81 1030.0 82 679.5 83 329.0 84 329.0 85 329.0 86 216.5 87 104.0 88 104.0 89 104.0 90 60.0 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 4.5 99 6.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21585366550972915 2 0.12908335096376516 3 0.13122759267412673 4 0.06432725131084643 5 0.018011630367037002 6 0.017439832577607256 7 0.3011944855821187 8 0.2963342043719659 9 0.015152641419888271 10 0.03473671570785707 11 0.012579551367454414 12 0.013866096393671342 13 0.012722500814811851 14 0.014151995288386215 15 0.01186480413066723 16 0.015581489761960582 17 0.014437894183101088 18 0.011578905235952357 19 0.018440478709109313 20 0.013866096393671342 21 0.011721854683309794 22 0.013437248051599031 23 0.014009045841028777 24 0.012150703025382105 25 0.01186480413066723 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 699548.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.25991227535827 #Duplication Level Percentage of deduplicated Percentage of total 1 93.5523002414379 79.76260911742975 2 4.821498703108726 8.221611129256072 3 0.8116526954542121 2.0760431283745264 4 0.28218501267335117 0.9623627770380313 5 0.14154039767991214 0.6033860944804316 6 0.081660055926001 0.4177397522791017 7 0.06025762507144784 0.3596291879259151 8 0.036005125774575056 0.24558350920028083 9 0.026204479308572514 0.20107724463633 >10 0.1563120978831473 2.465647824299633 >50 0.016115296428037284 0.9750954671043109 >100 0.012757511335604672 2.3494412799399123 >500 6.714479637441607E-4 0.41790743974995415 >1k 8.393099546802009E-4 0.9418660482857699 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1710 0.24444355498121645 No Hit CTGTAGGACGTGGAATATGGCAAGA 1359 0.19426829895875625 No Hit GTCCTACAGTGTGCATTTCTCATTT 1254 0.1792586069862254 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1231 0.17597076969700434 No Hit CTTTAGGACGTGAAATATGGCGAGG 1027 0.1468090824360873 No Hit CTGTAGGACCTGGAATATGGCGAGA 847 0.12107818191174874 No Hit GTATCAACGCAGAGTACTTTTTTTT 815 0.11650379959631076 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.429494473574365E-4 0.0 5 0.0 0.0 0.0 1.429494473574365E-4 0.0 6 0.0 0.0 0.0 1.429494473574365E-4 0.0 7 0.0 1.429494473574365E-4 0.0 1.429494473574365E-4 0.0 8 0.0 1.429494473574365E-4 0.0 1.429494473574365E-4 0.0 9 0.0 1.429494473574365E-4 0.0 2.85898894714873E-4 0.0 10 0.0 1.429494473574365E-4 0.0 2.85898894714873E-4 0.0 11 0.0 1.429494473574365E-4 0.0 2.85898894714873E-4 0.0 12 0.0 1.429494473574365E-4 0.0 2.85898894714873E-4 2.85898894714873E-4 13 0.0 1.429494473574365E-4 0.0 2.85898894714873E-4 2.85898894714873E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAAGTC 35 0.0021164685 16.345121 3 TCCAACG 60 1.4963043E-6 15.800212 18 CGGTTTC 50 8.859852E-5 15.168203 13 AATCCCG 70 4.5320303E-7 14.897344 19 TAGGACC 385 0.0 13.364634 4 GTATTAG 50 0.0014543893 13.352356 1 GTAGGAC 820 0.0 12.790389 3 TGTAGGA 875 0.0 12.749194 2 AAATCCC 90 5.529346E-7 12.640169 18 CTGTAGG 815 0.0 12.638513 1 GACGTGG 390 0.0 12.45049 7 CTAGACT 85 3.8049511E-6 12.330985 4 AGGACGT 695 0.0 12.193188 5 GGACGTG 680 0.0 12.182107 6 TATACTG 150 1.2732926E-11 12.060753 5 GTCCTAG 95 9.889573E-7 12.047237 1 TAGGACG 715 0.0 11.993901 4 GGCGAGG 325 0.0 11.959546 19 TCTAGGA 145 9.094947E-11 11.836122 2 AGGACCT 540 0.0 11.813896 5 >>END_MODULE