FastQCFastQC Report
Thu 26 May 2016
SRR1926865_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926865_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699548
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT16890.24144161658671026No Hit
CTGTAGGACGTGGAATATGGCAAGA15880.22700372240360916No Hit
GTATCAACGCAGAGTACTTTTTTTT14310.20456065916849164No Hit
GTCCTACAGTGTGCATTTCTCATTT12870.1839759387490208No Hit
GTCCTAAAGTGTGTATTTCTCATTT12290.17568487080228948No Hit
CTTTAGGACGTGAAATATGGCGAGG12100.17296883130249818No Hit
CTGTAGGACCTGGAATATGGCGAGA10610.15166936364624015No Hit
GGTATCAACGCAGAGTACTTTTTTT9230.13194233991091392No Hit
GTACATGGGAAGCAGTGGTATCAAC7870.11250121507030254No Hit
TATCAACGCAGAGTACTTTTTTTTT7540.10778388330750714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4050.014.1054234
TAAGACT852.6198722E-713.4416394
TGTAGGA9100.012.7774662
TAGACTG1207.1122486E-1012.69488055
GATATAC2100.012.2555581
GTAGGAC9550.012.1631583
AGGACCT6550.012.0649825
CTAGACT802.7966405E-511.9014514
ACTATAC802.7966405E-511.9014513
CTAAGAC802.7966405E-511.9014513
GGACCTG6650.011.8835546
ACTGTTC2400.011.8597668
CTGTAGG9700.011.792291
GGCGAGG3300.011.78957419
CCCTTAT657.7900547E-411.7318151
CTAGAAC1302.5138434E-911.7183513
GTAGACT657.865494E-411.7183514
TCTGTAC657.865494E-411.7183513
GACGTGG3650.011.6981397
GTCCTAC8350.011.6440031