FastQCFastQC Report
Thu 26 May 2016
SRR1926864_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926864_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628694
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT9710.154447155531944No Hit
CTGTAGGACGTGGAATATGGCAAGA8290.1318606508094558No Hit
GTCCTACAGTGTGCATTTCTCATTT8290.1318606508094558No Hit
GTCCTAAAGTGTGTATTTCTCATTT7570.1204083385557998No Hit
GTATCAACGCAGAGTACTTTTTTTT7180.11420500275173613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTAG706.989283E-613.6188951
TAATCTG707.1056675E-613.5971495
GACAGTC655.3465294E-513.1787757
CTACACT1750.013.0595194
TGTAGGA6000.012.3882412
TATACTG1001.4020588E-712.3734055
TAGGACC2650.012.2176314
GGCGAGG1405.4569682E-1112.19265419
CTGTAGG5800.012.1630831
GTAGGAC5900.011.9511953
TCTAGGA802.7708751E-511.911772
GTGTAGC1302.4610927E-911.7332021
TGGCGAG4050.011.707623518
CTAGGAG907.195207E-611.6461333
CCTACAC1604.3655746E-1111.3152783
TCTACAC1354.483809E-911.293223
GTATAGT600.005718431411.1220981
CTCCTAA600.005718431411.1220981
GACCTAC600.005718431411.1220981
TATAGGA1201.2165037E-711.1176532
Produced by FastQC (version 0.11.5)