##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926864_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628694 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.75863933805635 31.0 28.0 31.0 16.0 34.0 2 27.7261434020366 31.0 28.0 31.0 16.0 34.0 3 27.38141130661339 31.0 27.0 31.0 10.0 34.0 4 31.147249695400305 35.0 32.0 37.0 17.0 37.0 5 31.39313561128307 35.0 32.0 37.0 17.0 37.0 6 30.838245633010654 35.0 32.0 37.0 11.0 37.0 7 30.60717773670498 35.0 31.0 37.0 10.0 37.0 8 30.52398464117679 35.0 31.0 37.0 10.0 37.0 9 32.530698559235496 37.0 32.0 39.0 15.0 39.0 10 32.726272240549456 37.0 33.0 39.0 13.0 39.0 11 32.91323282868932 37.0 33.0 39.0 16.0 39.0 12 32.781590089932465 37.0 33.0 39.0 11.0 39.0 13 32.87996545219137 37.0 33.0 39.0 11.0 39.0 14 33.82667084463984 39.0 33.0 40.0 11.0 41.0 15 33.420043773282394 38.0 33.0 40.0 10.0 41.0 16 33.490702949288526 38.0 33.0 40.0 10.0 41.0 17 33.69732015893265 39.0 33.0 40.0 10.0 41.0 18 33.73276665595663 39.0 33.0 40.0 10.0 41.0 19 33.09318364737058 39.0 32.0 40.0 7.0 41.0 20 33.38709610716819 39.0 33.0 40.0 2.0 41.0 21 33.37284115961024 39.0 33.0 40.0 2.0 41.0 22 33.38334547490512 39.0 33.0 40.0 2.0 41.0 23 33.34215373456721 39.0 33.0 40.0 2.0 41.0 24 32.029838045217545 38.0 31.0 40.0 2.0 41.0 25 32.58264911069614 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5850835952309943 1101 2 -0.7867604595628812 1101 3 -1.9062451624070818 1101 4 -1.2631082052806981 1101 5 -0.79827113644237 1101 6 -3.0511998536480576 1101 7 -1.2211086015299806 1101 8 -0.33141213339431275 1101 9 -0.5649510885415694 1101 10 -9.407565075818042 1101 11 -4.237207592210879 1101 12 -1.7996066442267065 1101 13 -1.0895793326016694 1101 14 -0.9147407990444236 1101 15 -2.770063978627423 1101 16 -0.7740637379276549 1101 17 -0.6640480647305225 1101 18 -0.5318193697610738 1101 19 -1.0433389781549138 1101 20 -0.4584934443596467 1101 21 -0.5112255883742733 1101 22 -0.2003781256710795 1101 23 -0.41014558404579304 1101 24 -0.7974433901239486 1101 25 -0.5415883376226844 1102 1 -0.24001600129953005 1102 2 -0.4851297129311014 1102 3 -0.47027119008731333 1102 4 -0.5191824782729633 1102 5 0.11880178237605676 1102 6 -0.7137213712408439 1102 7 -0.2771059849906976 1102 8 0.49292053432814953 1102 9 0.07432110040011253 1102 10 0.15459265100607666 1102 11 -0.08849864777791083 1102 12 0.05089026871598179 1102 13 -0.07497835484225135 1102 14 -0.22536417805243047 1102 15 0.4178723981542376 1102 16 0.1564751773561497 1102 17 -0.2366076828075947 1102 18 -0.5048322917927948 1102 19 0.010204825925967498 1102 20 -0.031509809206212935 1102 21 -0.29850897794025144 1102 22 -0.29104350702019843 1102 23 -0.8962710402187568 1102 24 -4.159624069047034 1102 25 -2.3418970433538213 1103 1 0.23121993852971556 1103 2 -0.06211620613252933 1103 3 0.30910948480088507 1103 4 0.44654006769080823 1103 5 0.19729706177254158 1103 6 1.1383678910801613 1103 7 0.7433236125157379 1103 8 1.1042477241009294 1103 9 0.8372813027762191 1103 10 0.9106142438145 1103 11 0.7387679195076302 1103 12 0.320759718054191 1103 13 0.4868434819016585 1103 14 0.3018368880360143 1103 15 0.7046953642291314 1103 16 0.6146018431193454 1103 17 0.4884024811712635 1103 18 0.31617326451564765 1103 19 -1.3213915963534717 1103 20 -0.016433240565476126 1103 21 0.1258235874480107 1103 22 0.46976870782372515 1103 23 0.28841974895773603 1103 24 1.89153688320485 1103 25 1.1447294191298027 1104 1 -0.3829738118105297 1104 2 -0.30025519029062053 1104 3 -1.681118616892185 1104 4 -0.5612992267726895 1104 5 -0.3511609381185785 1104 6 -1.5442087325792677 1104 7 -0.11737239706562264 1104 8 0.3158474301273877 1104 9 0.23840547937810896 1104 10 0.5334979065070797 1104 11 0.37670411636102585 1104 12 0.4414521890651386 1104 13 0.100799059233033 1104 14 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-2.6176722115067648 2216 12 -2.303597195452941 2216 13 -2.016665544474453 2216 14 -2.110056737299878 2216 15 -3.4439185502086396 2216 16 -2.0535312823313667 2216 17 -2.3792113406056465 2216 18 -2.088255118713544 2216 19 -1.7580478286018675 2216 20 -2.6590987308421283 2216 21 -5.11908510132028 2216 22 -3.5367256096484887 2216 23 -3.4818618997609256 2216 24 -3.509684628617464 2216 25 -2.793584636716492 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 42294.0 3 3.0 4 291.0 5 1837.0 6 2384.0 7 1879.0 8 1483.0 9 1368.0 10 1205.0 11 1241.0 12 1300.0 13 1287.0 14 1253.0 15 1365.0 16 1385.0 17 1535.0 18 1706.0 19 2194.0 20 2796.0 21 3534.0 22 4414.0 23 5453.0 24 6329.0 25 7216.0 26 8138.0 27 8867.0 28 9892.0 29 11550.0 30 13677.0 31 16725.0 32 21416.0 33 27655.0 34 37318.0 35 51409.0 36 73003.0 37 112927.0 38 140365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80467575850786 18.14107600777115 12.249282411797612 25.804965821923375 2 14.974202178482706 19.298044461430663 41.467131664437225 24.260621695649405 3 19.308532662356274 23.701309042265184 28.52469981208396 28.465458483294583 4 11.910120944506879 16.53205395544887 37.62093654790822 33.93688855213603 5 12.939310340438666 37.57250907656131 36.222400412382946 13.265780170617077 6 33.20430579685243 36.341854560033596 17.742797686095575 12.711041957018399 7 29.372885168540403 30.611193271465787 21.55728172067713 18.45863983931668 8 25.158885124140966 37.84847846755617 19.972944862395273 17.01969154590759 9 26.267894018410274 15.552324193953817 19.60283949713791 38.576942290497996 10 14.751450752386363 29.15602569426875 34.24141601707011 21.851107536274778 11 34.97935054439474 22.454453473499335 23.641399454014394 18.92479652809153 12 23.269787886645908 24.573681271566503 31.49914330577382 20.657387536013772 13 29.13584371370846 21.674064175376635 25.310297650296697 23.879794460618207 14 20.76906800895353 21.932167635253485 27.086651001703842 30.212113354089144 15 23.398287922391756 30.75678539213768 22.63785947345898 23.207067212011587 16 22.30648810148815 28.060378703270615 27.476191839838616 22.156941355402616 17 21.956116403530544 28.671655753040227 27.330043336324238 22.042184507104995 18 21.918723502414114 27.74155060094337 28.87598533236822 21.463740564274296 19 24.086881675266056 26.740362081227236 26.762317968984924 22.41043827452178 20 23.676465441263034 27.30304081249543 27.548991221511276 21.471502524730266 21 23.000133631990224 26.78955506910683 26.729897930613177 23.480413368289767 22 23.16302748279839 27.928091317662968 26.809370401304538 22.0995107982341 23 22.483430749601478 27.61647793259028 27.294797430374146 22.605293887434097 24 23.65780594006481 26.88747158272866 27.51507765929183 21.939644817914694 25 23.011575149382427 27.025810228639426 27.419231674801303 22.543382947176845 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 150.0 1 150.0 2 227.5 3 305.0 4 305.0 5 305.0 6 425.5 7 546.0 8 546.0 9 546.0 10 822.0 11 1098.0 12 1098.0 13 1098.0 14 1775.5 15 2453.0 16 2453.0 17 2453.0 18 4407.5 19 6362.0 20 6362.0 21 6362.0 22 10039.0 23 13716.0 24 13716.0 25 13716.0 26 19577.0 27 25438.0 28 25438.0 29 25438.0 30 34139.0 31 42840.0 32 42840.0 33 42840.0 34 49709.5 35 56579.0 36 56579.0 37 56579.0 38 62498.5 39 68418.0 40 68418.0 41 68418.0 42 73165.5 43 77913.0 44 77913.0 45 77913.0 46 81142.0 47 84371.0 48 84371.0 49 84371.0 50 81924.5 51 79478.0 52 79478.0 53 79478.0 54 71621.0 55 63764.0 56 63764.0 57 63764.0 58 55006.0 59 46248.0 60 46248.0 61 46248.0 62 37877.5 63 29507.0 64 29507.0 65 29507.0 66 23130.5 67 16754.0 68 16754.0 69 16754.0 70 12332.0 71 7910.0 72 7910.0 73 7910.0 74 5502.5 75 3095.0 76 3095.0 77 3095.0 78 2145.5 79 1196.0 80 1196.0 81 1196.0 82 789.5 83 383.0 84 383.0 85 383.0 86 253.5 87 124.0 88 124.0 89 124.0 90 81.0 91 38.0 92 38.0 93 38.0 94 21.5 95 5.0 96 5.0 97 5.0 98 4.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1980295660528015 2 0.11674996103032637 3 0.11993115887856413 4 0.0750762692184115 5 0.024177103646607093 6 0.02322274429213576 7 0.2976010587026439 8 0.29044336354410893 9 0.02099590579836932 10 0.03610659557749875 11 0.01686034859566021 12 0.01829188762736721 13 0.016542228810836432 14 0.01829188762736721 15 0.016065049133600767 16 0.020359666228721766 17 0.01940530687425043 18 0.015746929348776986 19 0.025608642678314093 20 0.017814707950131542 21 0.016224109026012654 22 0.018928127197014765 23 0.019087187089426652 24 0.01829188762736721 25 0.01686034859566021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628694.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.97039082084162 #Duplication Level Percentage of deduplicated Percentage of total 1 93.51825193532441 81.3331891969709 2 4.870585779588673 8.471934975545208 3 0.8272001422692677 2.15825758980642 4 0.27824394163953303 0.9679593739168654 5 0.15185391353135833 0.6603397103748261 6 0.08645461543415359 0.45113950155443416 7 0.05349282193873325 0.32566041410849245 8 0.034720466280299185 0.24157220175035785 9 0.02318290885244418 0.1814603978934917 >10 0.13423138137017004 2.0903898545017516 >50 0.010982658367952529 0.6670219503142447 >100 0.009152063901252224 1.434954117595557 >500 0.001647371501746059 1.016120715667489 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 971 0.154447155531944 No Hit CTGTAGGACGTGGAATATGGCAAGA 829 0.1318606508094558 No Hit GTCCTACAGTGTGCATTTCTCATTT 829 0.1318606508094558 No Hit GTCCTAAAGTGTGTATTTCTCATTT 757 0.1204083385557998 No Hit GTATCAACGCAGAGTACTTTTTTTT 718 0.11420500275173613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 1.5905989241188877E-4 0.0 0.0 0.0 8 0.0 1.5905989241188877E-4 0.0 0.0 0.0 9 0.0 1.5905989241188877E-4 0.0 0.0 0.0 10 0.0 1.5905989241188877E-4 0.0 0.0 0.0 11 0.0 1.5905989241188877E-4 0.0 0.0 0.0 12 0.0 1.5905989241188877E-4 0.0 0.0 0.0 13 0.0 1.5905989241188877E-4 0.0 0.0 1.5905989241188877E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 70 6.989283E-6 13.618895 1 TAATCTG 70 7.1056675E-6 13.597149 5 GACAGTC 65 5.3465294E-5 13.178775 7 CTACACT 175 0.0 13.059519 4 TGTAGGA 600 0.0 12.388241 2 TATACTG 100 1.4020588E-7 12.373405 5 TAGGACC 265 0.0 12.217631 4 GGCGAGG 140 5.4569682E-11 12.192654 19 CTGTAGG 580 0.0 12.163083 1 GTAGGAC 590 0.0 11.951195 3 TCTAGGA 80 2.7708751E-5 11.91177 2 GTGTAGC 130 2.4610927E-9 11.733202 1 TGGCGAG 405 0.0 11.7076235 18 CTAGGAG 90 7.195207E-6 11.646133 3 CCTACAC 160 4.3655746E-11 11.315278 3 TCTACAC 135 4.483809E-9 11.29322 3 GTATAGT 60 0.0057184314 11.122098 1 CTCCTAA 60 0.0057184314 11.122098 1 GACCTAC 60 0.0057184314 11.122098 1 TATAGGA 120 1.2165037E-7 11.117653 2 >>END_MODULE