FastQCFastQC Report
Thu 26 May 2016
SRR1926864_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926864_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences628694
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12750.20280136282515818No Hit
GTCCTACAGTGGACATTTCTAAATT10210.16240015015253842No Hit
CTGTAGGACGTGGAATATGGCAAGA9880.15715117370294612No Hit
GTCCTACAGTGTGCATTTCTCATTT8500.13520090855010544No Hit
GGTATCAACGCAGAGTACTTTTTTT7920.1259754347902159No Hit
CTGTAGGACCTGGAATATGGCGAGA7550.12009021877097603No Hit
GTACATGGGAAGCAGTGGTATCAAC7200.11452312253655993No Hit
CTTTAGGACGTGAAATATGGCGAGG7000.11134192468832213No Hit
TATCAACGCAGAGTACTTTTTTTTT6850.10895602630214381No Hit
GTCCTAAAGTGTGTATTTCTCATTT6820.10847884662490814No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG250.0060575618.98400718
CTAGGAC902.215529E-914.8065753
TAGGACC2800.013.9378224
CTAATAC551.9251594E-413.8451093
GTGTAGA1700.012.8872421
TAATACT905.262027E-712.691354
ACTATAC604.0194165E-412.691353
GTCCTAA2200.012.5561481
TCTACAC701.0692874E-412.2380883
TTGCACA951.0123895E-612.0233844
CTTGCAC802.8035543E-511.8981413
TAGGACA2100.011.7848254
AGGACCT4850.011.7754795
CTGTAGG5950.011.5264791
GTGTAAC1001.8626506E-611.4304241
CTAGAAG752.0329584E-411.4222153
TAGGACT1001.8788269E-611.4222154
GGCGAGA4350.011.34676219
GGACCTG5050.011.1197516
TAATAGG600.00578459311.1049314