##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926864_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 628694 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95257788367632 31.0 30.0 33.0 26.0 34.0 2 30.245696634610795 31.0 30.0 34.0 26.0 34.0 3 30.31557959834196 31.0 30.0 34.0 26.0 34.0 4 33.94366893910233 37.0 35.0 37.0 30.0 37.0 5 33.94987227490639 37.0 35.0 37.0 30.0 37.0 6 33.908343327596576 37.0 35.0 37.0 30.0 37.0 7 33.89907808886358 37.0 35.0 37.0 30.0 37.0 8 33.92831806888566 37.0 35.0 37.0 30.0 37.0 9 35.47696653697984 39.0 35.0 39.0 30.0 39.0 10 35.359376739717575 39.0 35.0 39.0 30.0 39.0 11 35.40399622073696 39.0 35.0 39.0 30.0 39.0 12 35.30288502832857 39.0 35.0 39.0 30.0 39.0 13 35.31771418209813 39.0 35.0 39.0 30.0 39.0 14 36.434247821674774 40.0 37.0 41.0 30.0 41.0 15 36.40838945496537 40.0 36.0 41.0 30.0 41.0 16 36.39703416924609 40.0 36.0 41.0 30.0 41.0 17 36.36359500806434 40.0 36.0 41.0 30.0 41.0 18 36.33466837603031 40.0 36.0 41.0 30.0 41.0 19 36.39839413132621 40.0 37.0 41.0 30.0 41.0 20 36.369381606950284 40.0 37.0 41.0 30.0 41.0 21 36.31332571966648 40.0 37.0 41.0 29.0 41.0 22 36.22793759762301 40.0 37.0 41.0 29.0 41.0 23 36.160928846147726 40.0 37.0 41.0 29.0 41.0 24 36.095019198529016 40.0 37.0 41.0 29.0 41.0 25 35.97166666136467 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.03767476526778779 1101 2 0.1298431425146802 1101 3 0.3017215124559023 1101 4 0.24329987885186455 1101 5 0.17728715013377894 1101 6 0.1283138384013114 1101 7 0.15392622365019548 1101 8 0.042306502886305 1101 9 0.21422720725576028 1101 10 0.17922102637719206 1101 11 0.12004439523648358 1101 12 0.0685756652908509 1101 13 0.2910869110442249 1101 14 0.1404474770399844 1101 15 0.13225516297450923 1101 16 0.02443827306080948 1101 17 0.1364220023678584 1101 18 0.28291458518656043 1101 19 0.1426273488166956 1101 20 0.34386564014817367 1101 21 0.12859767168070135 1101 22 0.2240780917614984 1101 23 0.14907974441057803 1101 24 0.20230595533736562 1101 25 0.32463856542131 1102 1 -0.3547590753069336 1102 2 -0.5583273456764637 1102 3 -0.32919726006584327 1102 4 -0.4871047666529691 1102 5 -0.4662908525745735 1102 6 -0.3857546506620082 1102 7 -0.40423669034828436 1102 8 -0.48791796864161796 1102 9 -0.3578481215330527 1102 10 -0.4365550988494604 1102 11 -0.3380909762432367 1102 12 -0.43558210743746173 1102 13 -0.468435914818194 1102 14 -0.5640740864569906 1102 15 -0.6112551310418723 1102 16 -0.5090844150890206 1102 17 -0.5287872060142433 1102 18 -0.4784524418280611 1102 19 -0.6774339332875527 1102 20 -0.5814459345953367 1102 21 -0.748392849102423 1102 22 -0.7500043980821971 1102 23 -0.6738884210281526 1102 24 -0.7768960001814662 1102 25 -0.617344266408665 1103 1 -0.24668887609294998 1103 2 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0.18577344518238803 2214 2 0.17379345048001937 2214 3 0.13495147423913423 2214 4 0.19945908332078943 2214 5 0.2748391890096684 2214 6 -0.038851263363369526 2214 7 -0.049656368171369536 2214 8 0.05061204871878999 2214 9 0.07808994621291987 2214 10 0.0618400725341246 2214 11 -0.07818948647780388 2214 12 0.01644058221492628 2214 13 0.12535672921264762 2214 14 0.043834993441265624 2214 15 -0.0028534999707758857 2214 16 -0.022849605447937904 2214 17 0.13972989197113606 2214 18 -0.07104802501672935 2214 19 0.10468282556423958 2214 20 0.09728150696467708 2214 21 0.13574054344471165 2214 22 0.12154067842472216 2214 23 0.13667720535967476 2214 24 -0.1313774083933481 2214 25 0.08475311338058589 2215 1 0.08292447279015747 2215 2 0.03518978547926466 2215 3 -0.013394642944675184 2215 4 0.19128900020348993 2215 5 0.10231867694295715 2215 6 0.19274400082135656 2215 7 -0.05935605904272023 2215 8 0.06507873498438954 2215 9 -0.177221810475217 2215 10 0.0407248931593287 2215 11 -0.0964467848537609 2215 12 -0.01039481346084159 2215 13 -0.10084526453351117 2215 14 -0.14684304060742903 2215 15 -0.18158776514436425 2215 16 -0.17415783432785759 2215 17 0.08962254326154806 2215 18 0.053262671547223306 2215 19 -0.08347229109396892 2215 20 0.05903953139207374 2215 21 -0.07423442337073283 2215 22 -0.029297176026460647 2215 23 -0.04351183527248281 2215 24 0.10125303587886236 2215 25 0.011239291677441088 2216 1 -0.19278442958042064 2216 2 0.039068592795011625 2216 3 -1.6259593417001454 2216 4 -1.4665750722769317 2216 5 -0.5363562569106364 2216 6 -0.05972412863851417 2216 7 -0.0249884364825661 2216 8 0.08381887983833991 2216 9 0.004086151146502459 2216 10 -0.23227757452469433 2216 11 -0.10285741816660732 2216 12 0.02403071504225096 2216 13 -0.07388425750461636 2216 14 0.020115828355876886 2216 15 -0.16034875613776478 2216 16 -0.07977560165286945 2216 17 -0.13256612320912353 2216 18 0.034265067962401474 2216 19 -0.06324886324031809 2216 20 -0.1465515101131203 2216 21 -0.20676420038830656 2216 22 -0.2750437618029764 2216 23 -0.5189205176004776 2216 24 -0.41980245583167886 2216 25 -0.35832089041447546 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13780.0 3 11.0 4 231.0 5 1543.0 6 2009.0 7 1476.0 8 1172.0 9 1026.0 10 879.0 11 834.0 12 727.0 13 720.0 14 741.0 15 773.0 16 746.0 17 804.0 18 920.0 19 1095.0 20 1411.0 21 1786.0 22 2323.0 23 2879.0 24 3373.0 25 4070.0 26 4800.0 27 5277.0 28 6336.0 29 7880.0 30 10075.0 31 12922.0 32 16793.0 33 21963.0 34 30334.0 35 42423.0 36 62304.0 37 106985.0 38 255273.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32819230677357 18.930018870778955 10.420332667149717 28.321456155297753 2 14.6130281376183 20.190874699071422 39.60188263466952 25.59421452864076 3 18.948207374129616 23.80071364597369 28.884844647575658 28.36623433232103 4 12.280537113444696 16.686178013469192 37.1735693357977 33.85971553728841 5 13.239897688446048 38.23973862197535 34.975694643277535 13.544669046301069 6 32.973899553261475 37.126700407475326 17.070072030401843 12.829328008861351 7 29.010575015471947 31.284036292651262 20.863660734030486 18.841727957846306 8 25.629306998733753 37.86802369604917 19.004562316664227 17.49810698855285 9 26.2581785383065 15.576009302813908 19.202165990324946 38.96364616855465 10 14.912075588747584 29.546260727096307 33.4941503026254 22.047513381530703 11 35.1402511381387 22.537850468776348 23.442953084188858 18.878945308896093 12 23.52041076018629 24.61045860585855 31.122750871656528 20.746379762298627 13 29.358216979541456 21.722290618846294 25.142917131922772 23.776575269689477 14 20.903048069566545 22.032693222288145 26.88211893563313 30.18213977251218 15 23.305841651091583 30.795721159581657 22.590368632441248 23.308068556885512 16 22.466469854681495 28.168980479983713 27.15878146234698 22.205768202987812 17 21.8261435267882 28.807474578131387 27.214959336144467 22.15142255893595 18 21.873136002061432 27.62987741156597 28.953902485966832 21.543084100405764 19 23.64977389528401 26.827169467989837 27.049059076716542 22.473997560009607 20 23.698634930633098 27.245715667280646 27.5380714697003 21.51757793238596 21 23.03684743859158 26.552808616984215 26.860308072139087 23.550035872285118 22 23.27638449927071 27.79671477175664 26.743577548978596 22.18332317999405 23 22.413765679328897 27.702188982239374 27.1607491084693 22.72329622996243 24 23.179871860943038 26.735030825905216 27.942776431066406 22.142320882085347 25 22.831555757040714 27.27000866916402 27.40442047831516 22.494015095480105 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 194.0 1 194.0 2 188.0 3 182.0 4 182.0 5 182.0 6 311.0 7 440.0 8 440.0 9 440.0 10 682.0 11 924.0 12 924.0 13 924.0 14 1545.5 15 2167.0 16 2167.0 17 2167.0 18 4003.0 19 5839.0 20 5839.0 21 5839.0 22 9471.0 23 13103.0 24 13103.0 25 13103.0 26 19205.0 27 25307.0 28 25307.0 29 25307.0 30 33740.5 31 42174.0 32 42174.0 33 42174.0 34 48783.5 35 55393.0 36 55393.0 37 55393.0 38 61330.0 39 67267.0 40 67267.0 41 67267.0 42 72385.0 43 77503.0 44 77503.0 45 77503.0 46 81283.0 47 85063.0 48 85063.0 49 85063.0 50 83453.5 51 81844.0 52 81844.0 53 81844.0 54 73282.0 55 64720.0 56 64720.0 57 64720.0 58 55924.0 59 47128.0 60 47128.0 61 47128.0 62 38621.0 63 30114.0 64 30114.0 65 30114.0 66 23477.5 67 16841.0 68 16841.0 69 16841.0 70 12290.0 71 7739.0 72 7739.0 73 7739.0 74 5432.5 75 3126.0 76 3126.0 77 3126.0 78 2135.0 79 1144.0 80 1144.0 81 1144.0 82 752.0 83 360.0 84 360.0 85 360.0 86 232.0 87 104.0 88 104.0 89 104.0 90 59.5 91 15.0 92 15.0 93 15.0 94 8.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1175452604923858 2 0.10148021135878504 3 0.014474450209481878 4 0.0 5 0.004135557202709108 6 0.3424559483627965 7 0.02179120526042876 8 0.01049795289918466 9 0.010657012791596547 10 0.004612736879944774 11 0.005726156126827996 12 9.543593544713326E-4 13 0.0012724791392951104 14 1.590598924118888E-4 15 0.0030221379558258866 16 9.543593544713326E-4 17 4.771796772356663E-4 18 7.952994620594438E-4 19 4.771796772356663E-4 20 0.0012724791392951104 21 0.011929491930891658 22 1.590598924118888E-4 23 0.0 24 3.181197848237776E-4 25 0.004612736879944774 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 628694.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.817097147871 #Duplication Level Percentage of deduplicated Percentage of total 1 91.51521214978202 76.70539427266304 2 6.3642483401962195 10.668656428068067 3 1.1032283092624469 2.774081831211959 4 0.3994711326653128 1.3393004293751436 5 0.17878417810045297 0.7492585412173969 6 0.10866350456458658 0.5464715711110847 7 0.0635422016440492 0.37281460217322165 8 0.04741485506924667 0.3179340410873018 9 0.03571308929871795 0.2694030727681111 >10 0.15828090197248337 2.3863625115838003 >50 0.011961314750957906 0.689697445704895 >100 0.011391568474068513 1.740615351014085 >500 0.0017087352705448826 1.0746366303849764 >1k 3.797189490099739E-4 0.36537327163688815 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1275 0.20280136282515818 No Hit GTCCTACAGTGGACATTTCTAAATT 1021 0.16240015015253842 No Hit CTGTAGGACGTGGAATATGGCAAGA 988 0.15715117370294612 No Hit GTCCTACAGTGTGCATTTCTCATTT 850 0.13520090855010544 No Hit GGTATCAACGCAGAGTACTTTTTTT 792 0.1259754347902159 No Hit CTGTAGGACCTGGAATATGGCGAGA 755 0.12009021877097603 No Hit GTACATGGGAAGCAGTGGTATCAAC 720 0.11452312253655993 No Hit CTTTAGGACGTGAAATATGGCGAGG 700 0.11134192468832213 No Hit TATCAACGCAGAGTACTTTTTTTTT 685 0.10895602630214381 No Hit GTCCTAAAGTGTGTATTTCTCATTT 682 0.10847884662490814 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 1.5905989241188877E-4 0.0 7 0.0 0.0 0.0 1.5905989241188877E-4 0.0 8 0.0 0.0 0.0 1.5905989241188877E-4 0.0 9 0.0 0.0 0.0 1.5905989241188877E-4 0.0 10 0.0 0.0 0.0 1.5905989241188877E-4 0.0 11 0.0 0.0 0.0 1.5905989241188877E-4 0.0 12 0.0 0.0 0.0 1.5905989241188877E-4 0.0 13 0.0 0.0 0.0 1.5905989241188877E-4 1.5905989241188877E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGTCGAG 25 0.00605756 18.984007 18 CTAGGAC 90 2.215529E-9 14.806575 3 TAGGACC 280 0.0 13.937822 4 CTAATAC 55 1.9251594E-4 13.845109 3 GTGTAGA 170 0.0 12.887242 1 TAATACT 90 5.262027E-7 12.69135 4 ACTATAC 60 4.0194165E-4 12.69135 3 GTCCTAA 220 0.0 12.556148 1 TCTACAC 70 1.0692874E-4 12.238088 3 TTGCACA 95 1.0123895E-6 12.023384 4 CTTGCAC 80 2.8035543E-5 11.898141 3 TAGGACA 210 0.0 11.784825 4 AGGACCT 485 0.0 11.775479 5 CTGTAGG 595 0.0 11.526479 1 GTGTAAC 100 1.8626506E-6 11.430424 1 CTAGAAG 75 2.0329584E-4 11.422215 3 TAGGACT 100 1.8788269E-6 11.422215 4 GGCGAGA 435 0.0 11.346762 19 GGACCTG 505 0.0 11.119751 6 TAATAGG 60 0.005784593 11.104931 4 >>END_MODULE