FastQCFastQC Report
Thu 26 May 2016
SRR1926863_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926863_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640784
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17700.27622412544632824No Hit
CTGTAGGACGTGGAATATGGCAAGA14310.22332018277609927No Hit
GTCCTAAAGTGTGTATTTCTCATTT13410.2092748882618792No Hit
GTCCTACAGTGTGCATTTCTCATTT12090.1886751229743564No Hit
CTTTAGGACGTGAAATATGGCGAGG10750.16776324003096207No Hit
CTGTAGGACCTGGAATATGGCGAGA8780.13701965092761367No Hit
GTATCAACGCAGAGTACTTTTTTTT6780.10580788534045794No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTACG359.7691576E-519.0399634
TCCAACG602.6038826E-514.22646318
AATCCCG1006.730261E-1014.22646219
CCCCTAC551.8973072E-413.868981
CTAAGAC903.4691766E-813.7683453
TAGGACC3050.013.7337444
TCTAGAC701.0553698E-412.2553413
ATAGTAC701.0553698E-412.2553413
TAGAACT701.0678075E-412.2399774
GTCCTAC9150.011.8795781
TGTAGGA8450.011.8444112
GTCCTAG1052.5839472E-711.8051431
CTGTAGG8700.011.7268611
AAATCCC1302.6684575E-911.67299618
GGACCTG5650.011.4513966
GTCTTAT752.000785E-411.4419091
AGGACCT5900.011.2887015
ATCAGGA855.1327308E-511.2140372
CTATAAG600.005711060511.1240781
CTAAGTT600.005778150611.1066454
Produced by FastQC (version 0.11.5)