##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926863_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640784 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.84866195160928 31.0 28.0 31.0 16.0 34.0 2 27.851583372868237 31.0 28.0 31.0 16.0 34.0 3 27.4904741691428 31.0 27.0 31.0 10.0 34.0 4 31.22592168343779 35.0 32.0 37.0 17.0 37.0 5 31.451844927463856 35.0 32.0 37.0 17.0 37.0 6 30.888882993333166 35.0 32.0 37.0 11.0 37.0 7 30.674659479637445 35.0 31.0 37.0 11.0 37.0 8 30.608563884241804 35.0 31.0 37.0 10.0 37.0 9 32.5913115808135 37.0 32.0 39.0 15.0 39.0 10 32.763712889210716 37.0 33.0 39.0 13.0 39.0 11 32.947974668531046 37.0 33.0 39.0 16.0 39.0 12 32.831270755824114 37.0 33.0 39.0 11.0 39.0 13 32.9320036080801 38.0 33.0 39.0 11.0 39.0 14 33.889571212764366 39.0 33.0 40.0 11.0 41.0 15 33.501683874753425 39.0 33.0 40.0 10.0 41.0 16 33.56954917725786 39.0 33.0 40.0 10.0 41.0 17 33.78162532148119 39.0 33.0 40.0 10.0 41.0 18 33.810738095832605 39.0 33.0 41.0 10.0 41.0 19 33.17716578441409 39.0 32.0 40.0 7.0 41.0 20 33.493738919823215 39.0 33.0 40.0 2.0 41.0 21 33.47863398586731 39.0 33.0 40.0 2.0 41.0 22 33.483250205997656 39.0 33.0 41.0 2.0 41.0 23 33.46873205323479 39.0 34.0 41.0 2.0 41.0 24 32.15170478663637 38.0 31.0 40.0 2.0 41.0 25 32.71797360733102 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.47556232641616347 1101 2 -0.5686091732114242 1101 3 -1.393514236280538 1101 4 -0.8454879361198628 1101 5 -0.7378189418886265 1101 6 -2.3528176469788775 1101 7 -1.2047896733052461 1101 8 -0.5767800337398654 1101 9 -0.5022808876427334 1101 10 -8.812450577763304 1101 11 -3.9906916944399775 1101 12 -1.843342238107624 1101 13 -1.143570744834367 1101 14 -1.061059889188435 1101 15 -2.7984936191947583 1101 16 -0.9895674526237173 1101 17 -0.859507120832312 1101 18 -0.7571867319061667 1101 19 -1.263132838821857 1101 20 -0.6628731637041412 1101 21 -0.3375554876450977 1101 22 -0.39041566468399935 1101 23 -0.5582575525546147 1101 24 -0.8496469602562726 1101 25 -0.6013841137119584 1102 1 -0.6869612901467335 1102 2 -0.948920054040439 1102 3 -1.3448095730681011 1102 4 -1.1822755008867034 1102 5 -0.706730858987072 1102 6 -1.6750974397249898 1102 7 -0.7809554764140536 1102 8 -0.23377485239271678 1102 9 -0.6411409912696762 1102 10 -0.5502744119601957 1102 11 -0.7948367721601812 1102 12 -0.476502859869278 1102 13 -0.6679230764405801 1102 14 -1.0714225834889533 1102 15 -0.4845039818890555 1102 16 -0.6330907686858964 1102 17 -0.8253102296405999 1102 18 -0.7385338821652354 1102 19 -0.36157843467677964 1102 20 -0.5768628010098382 1102 21 -0.6743430524119418 1102 22 -0.40595970613477306 1102 23 -0.8867549618810386 1102 24 -4.179180639012749 1102 25 -2.753715719929577 1103 1 -0.27106580896096943 1103 2 -0.6365920577778041 1103 3 -0.2955071437807497 1103 4 -0.6033502057197957 1103 5 -0.4900571139773042 1103 6 0.07530177882593136 1103 7 -0.18046070023126148 1103 8 0.23933947700505698 1103 9 -0.04888115945110627 1103 10 -0.19637990758577928 1103 11 -0.4187835147066892 1103 12 -0.6530954870671053 1103 13 -0.463470090795461 1103 14 -0.4980077784265262 1103 15 -0.22987156024631616 1103 16 -0.21275185761819415 1103 17 -0.26065593591767566 1103 18 -0.4763470988474694 1103 19 -2.559516513331328 1103 20 -1.1594458299744232 1103 21 -1.0227323754392046 1103 22 -0.9411026178819952 1103 23 -0.9038682719974105 1103 24 0.8067320425467557 1103 25 0.2793397884370208 1104 1 -0.2162970883085258 1104 2 -0.15467942082253572 1104 3 -1.4374020692276552 1104 4 -0.4402076486722635 1104 5 -0.11100612130904608 1104 6 -1.2048654507646432 1104 7 -0.07044957905159421 1104 8 0.25811985430137696 1104 9 0.3911447899681164 1104 10 0.528029022309596 1104 11 0.45905444558658104 1104 12 0.5959839263936431 1104 13 0.3437154269572318 1104 14 0.20044452406956026 1104 15 0.7182038578276604 1104 16 0.17865188239333207 1104 17 0.2614031299032291 1104 18 0.10868667658707665 1104 19 -0.2782186304744201 1104 20 0.21217044813744934 1104 21 0.4355848253186849 1104 22 0.43972467431670736 1104 23 0.3441196182736235 1104 24 1.7119589969911075 1104 25 0.9726310397756848 1105 1 -0.04612432743047279 1105 2 -0.37377692651446637 1105 3 0.22487196491885797 1105 4 -0.10285083432809117 1105 5 -0.13675443908358886 1105 6 -0.13944652972148575 1105 7 -0.19714156572563013 1105 8 0.4231143525240739 1105 9 -0.45838844634462816 1105 10 -0.133667959065491 1105 11 -0.34747162573876267 1105 12 -0.3779077316949895 1105 13 -0.2967462152599083 1105 14 -0.5962391972616174 1105 15 -0.06183153609452319 1105 16 -0.5043392589845936 1105 17 -0.32355191778729164 1105 18 -0.20938501385301578 1105 19 -1.0969125034721046 1105 20 -0.346849677718879 1105 21 -0.4365417003469787 1105 22 -0.4241953293342462 1105 23 -0.8149512152076142 1105 24 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1110 8 0.9004567855057211 1110 9 0.5060021140639392 1110 10 0.6562569469426265 1110 11 0.4785513383343414 1110 12 0.6158078983242987 1110 13 0.4727223947349799 1110 14 0.3253848073399581 1110 15 0.6968247730546864 1110 16 0.48536790828215004 1110 17 -0.09507650693976188 1110 18 0.3808690876562295 1110 19 -1.926594632773888 1110 20 -0.08299968259510848 1110 21 0.008876332162564893 1110 22 0.33487993781367464 1110 23 0.24652216354435552 1110 24 -0.592280147449042 1110 25 -0.21410243033621867 1111 1 0.1137208620434258 1111 2 -0.24984547373959032 1111 3 0.3854644414476951 1111 4 0.22334924518715837 1111 5 0.23933999455585564 1111 6 0.4457769188763976 1111 7 0.4084872152782353 1111 8 0.8599197540907397 1111 9 0.012568462055561724 1111 10 0.05759943001122281 1111 11 -0.24419850843813862 1111 12 -0.00982148011615891 1111 13 -0.016762665343570404 1111 14 -0.03442585882263671 1111 15 -1.0110863492581075 1111 16 -0.7148426960166248 1111 17 -0.5168550535600644 1111 18 -0.3008138755443426 1111 19 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5 -0.6218217826272188 2212 6 0.4621142353105192 2212 7 0.4130499650007131 2212 8 0.27745106634615624 2212 9 0.02033999378643614 2212 10 0.24530943984127163 2212 11 0.10503919559143071 2212 12 -0.22853538832667653 2212 13 -0.09484707668064374 2212 14 -0.39606699103492105 2212 15 -1.0753245952485315 2212 16 -0.2753507562826698 2212 17 -0.3034355422218695 2212 18 -0.312024989853235 2212 19 0.5242308757439318 2212 20 -0.7162690466337054 2212 21 -0.7894699854145202 2212 22 -0.1814303284965888 2212 23 -0.26731690798702346 2212 24 1.0434242382553407 2212 25 0.539423496266636 2213 1 -0.23483614373893502 2213 2 -0.1385980561447404 2213 3 0.46792244606913513 2213 4 -7.004074877536894 2213 5 -1.4722818486045277 2213 6 -2.7984184648773542 2213 7 -0.9459198422449546 2213 8 -2.2734816397589626 2213 9 -0.5268426568444866 2213 10 -0.1217928841575997 2213 11 0.11017980418031925 2213 12 0.22630122168234124 2213 13 0.1790068264836009 2213 14 -0.0619681138325916 2213 15 -2.403506225154299 2213 16 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0.21642227084189258 2215 3 0.31912963509548575 2215 4 0.6762064830550756 2215 5 0.717567088250199 2215 6 1.3576350287320729 2215 7 0.6123374476851424 2215 8 -0.2604884544177928 2215 9 0.029034620801674293 2215 10 -0.023185063386051752 2215 11 -0.061897643101133326 2215 12 -0.49163259130937575 2215 13 0.016178889307148836 2215 14 0.055935720509779685 2215 15 0.12941545014037104 2215 16 0.20363845648516588 2215 17 -0.1661746444647889 2215 18 -0.9650062153535401 2215 19 0.19059298485412768 2215 20 -4.098799477828258 2215 21 -2.1181600440664106 2215 22 -1.0135982379545752 2215 23 -0.8500890203392899 2215 24 -0.8739399634600034 2215 25 -0.2871334314353362 2216 1 -0.36361832585659926 2216 2 -1.0061903503403222 2216 3 -0.0879643301689157 2216 4 -0.3949689943414185 2216 5 -0.5410504238184792 2216 6 -1.7213335649597106 2216 7 -9.550630337464636 2216 8 -10.871603528970123 2216 9 -5.604804843975224 2216 10 -3.2984856904024547 2216 11 -2.1392204178270617 2216 12 -1.6823266295010981 2216 13 -1.744114167536523 2216 14 -1.607560193182323 2216 15 -2.755441628169301 2216 16 -1.555256496468786 2216 17 -1.8876348951128143 2216 18 -1.4901423595593855 2216 19 -0.9164377635228433 2216 20 -1.7918913979569915 2216 21 -4.882399697503111 2216 22 -3.0910726143071017 2216 23 -2.731803089762927 2216 24 -2.890546782164275 2216 25 -2.3269739425607128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 43490.0 3 3.0 4 371.0 5 1855.0 6 2429.0 7 1876.0 8 1508.0 9 1360.0 10 1284.0 11 1210.0 12 1178.0 13 1197.0 14 1305.0 15 1314.0 16 1364.0 17 1521.0 18 1731.0 19 2159.0 20 2772.0 21 3635.0 22 4354.0 23 5377.0 24 6070.0 25 6938.0 26 7957.0 27 8704.0 28 9557.0 29 11477.0 30 13593.0 31 16483.0 32 21251.0 33 27791.0 34 36984.0 35 51106.0 36 73548.0 37 114565.0 38 151467.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.84820784971675 18.79150450558918 12.61846423752109 25.74182340717298 2 14.134955828263038 19.767906975290703 42.95439701750932 23.14274017893694 3 19.131535360480015 24.22466327072721 28.974967967748995 27.668833401043784 4 11.498403129709596 17.024433286741683 38.745305045173076 32.73185853837564 5 12.353757467247135 37.9747467731034 36.992434022081355 12.679061737568118 6 32.04827627113882 37.43674353699235 18.269215760341908 12.245764431526927 7 28.39143177703467 30.89292224974533 22.35004905650339 18.36559691671661 8 24.00240958200011 39.117999108141724 20.642294422756468 16.237296887101696 9 26.215621390441108 15.525239596665937 20.366028782817718 37.89311023007523 10 14.445624004745996 29.55256502326162 35.06869204109033 20.93311893090205 11 34.09903617278651 22.51828149998049 24.987708276427206 18.39497405080579 12 23.422235194772963 24.70901510764529 32.289996862654505 19.57875283492724 13 29.038033205499808 22.45484073878101 25.548443287335115 22.95868276838407 14 19.999906347749537 23.000212278434383 27.704520906304047 29.295360467512033 15 22.54105013423238 32.550103015545986 22.72054691889867 22.18829993132297 16 21.234494287841585 28.42889498338409 29.30519112590162 21.031419602872703 17 21.089222191524666 29.59858520679488 28.27213853235497 21.040054069325492 18 20.859308420142412 28.00486359762627 30.836083024545875 20.299744957685455 19 23.82670184037588 26.982657696330175 27.076315501927787 22.114324961366155 20 23.05983754456286 28.177776945313326 28.439066724106716 20.323318786017094 21 22.356870824748704 27.23902728351127 26.999750265343074 23.404351626396952 22 22.46903212916599 29.148040158335725 27.42279884619707 20.96012886630121 23 21.425706102253127 28.715787502048652 28.109230252940304 21.749276142757914 24 23.324020166388312 27.355736962867 28.627062294160798 20.693180576583888 25 22.17426932532095 27.942404495258906 28.407538923791314 21.475787255628827 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 184.0 2 229.5 3 275.0 4 275.0 5 275.0 6 409.5 7 544.0 8 544.0 9 544.0 10 835.5 11 1127.0 12 1127.0 13 1127.0 14 1923.0 15 2719.0 16 2719.0 17 2719.0 18 4862.5 19 7006.0 20 7006.0 21 7006.0 22 11415.0 23 15824.0 24 15824.0 25 15824.0 26 23417.0 27 31010.0 28 31010.0 29 31010.0 30 41390.5 31 51771.0 32 51771.0 33 51771.0 34 59158.5 35 66546.0 36 66546.0 37 66546.0 38 71696.0 39 76846.0 40 76846.0 41 76846.0 42 80193.0 43 83540.0 44 83540.0 45 83540.0 46 84661.5 47 85783.0 48 85783.0 49 85783.0 50 80923.0 51 76063.0 52 76063.0 53 76063.0 54 66179.5 55 56296.0 56 56296.0 57 56296.0 58 47696.5 59 39097.0 60 39097.0 61 39097.0 62 31537.5 63 23978.0 64 23978.0 65 23978.0 66 18484.5 67 12991.0 68 12991.0 69 12991.0 70 9338.0 71 5685.0 72 5685.0 73 5685.0 74 3962.5 75 2240.0 76 2240.0 77 2240.0 78 1559.5 79 879.0 80 879.0 81 879.0 82 572.5 83 266.0 84 266.0 85 266.0 86 172.5 87 79.0 88 79.0 89 79.0 90 52.5 91 26.0 92 26.0 93 26.0 94 15.5 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19429324078004445 2 0.12203800344577892 3 0.12547129766036605 4 0.0731915903018802 5 0.023877000674174136 6 0.021536118255137456 7 0.2692014781892182 8 0.26046218382481456 9 0.019351294664036556 10 0.03807835401633 11 0.017010412244999877 12 0.017946765212614547 13 0.016698294589128318 14 0.018102824040550324 15 0.01623011810532098 16 0.020755824115458562 17 0.019507353491972336 18 0.01654223576119254 19 0.024033059502109915 20 0.018102824040550324 21 0.01623011810532098 22 0.01872705935229344 23 0.018571000524357662 24 0.017790706384678768 25 0.017010412244999877 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 640784.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 85.38176831182992 #Duplication Level Percentage of deduplicated Percentage of total 1 93.96844983225277 80.23192412199218 2 4.519689340104438 7.717981361564889 3 0.7410273934787698 1.8981068766817062 4 0.2568717212443094 0.8772864715657032 5 0.12459135753197595 0.5318915211225766 6 0.08185017939332366 0.4193107831945486 7 0.05007463335871058 0.2992822520613279 8 0.03885460977027139 0.26539802314014993 9 0.02872419960434272 0.22072706600046718 >10 0.15828458843809137 2.5637037869220567 >50 0.015517358006259137 0.9308556415015943 >100 0.01423924286121776 2.481888888435928 >500 9.1277197777994E-4 0.4976870701839639 >1k 9.1277197777994E-4 1.0639561356328648 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1770 0.27622412544632824 No Hit CTGTAGGACGTGGAATATGGCAAGA 1431 0.22332018277609927 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1341 0.2092748882618792 No Hit GTCCTACAGTGTGCATTTCTCATTT 1209 0.1886751229743564 No Hit CTTTAGGACGTGAAATATGGCGAGG 1075 0.16776324003096207 No Hit CTGTAGGACCTGGAATATGGCGAGA 878 0.13701965092761367 No Hit GTATCAACGCAGAGTACTTTTTTTT 678 0.10580788534045794 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.5605882793577868E-4 2 0.0 0.0 0.0 0.0 1.5605882793577868E-4 3 0.0 0.0 0.0 0.0 1.5605882793577868E-4 4 0.0 0.0 0.0 0.0 1.5605882793577868E-4 5 0.0 0.0 0.0 0.0 1.5605882793577868E-4 6 0.0 0.0 0.0 0.0 1.5605882793577868E-4 7 0.0 0.0 0.0 0.0 1.5605882793577868E-4 8 0.0 0.0 0.0 0.0 1.5605882793577868E-4 9 0.0 0.0 0.0 1.5605882793577868E-4 1.5605882793577868E-4 10 0.0 0.0 0.0 1.5605882793577868E-4 1.5605882793577868E-4 11 0.0 0.0 0.0 1.5605882793577868E-4 1.5605882793577868E-4 12 0.0 0.0 0.0 3.1211765587155736E-4 1.5605882793577868E-4 13 0.0 0.0 0.0 3.1211765587155736E-4 1.5605882793577868E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTACG 35 9.7691576E-5 19.039963 4 TCCAACG 60 2.6038826E-5 14.226463 18 AATCCCG 100 6.730261E-10 14.226462 19 CCCCTAC 55 1.8973072E-4 13.86898 1 CTAAGAC 90 3.4691766E-8 13.768345 3 TAGGACC 305 0.0 13.733744 4 TCTAGAC 70 1.0553698E-4 12.255341 3 ATAGTAC 70 1.0553698E-4 12.255341 3 TAGAACT 70 1.0678075E-4 12.239977 4 GTCCTAC 915 0.0 11.879578 1 TGTAGGA 845 0.0 11.844411 2 GTCCTAG 105 2.5839472E-7 11.805143 1 CTGTAGG 870 0.0 11.726861 1 AAATCCC 130 2.6684575E-9 11.672996 18 GGACCTG 565 0.0 11.451396 6 GTCTTAT 75 2.000785E-4 11.441909 1 AGGACCT 590 0.0 11.288701 5 ATCAGGA 85 5.1327308E-5 11.214037 2 CTATAAG 60 0.0057110605 11.124078 1 CTAAGTT 60 0.0057781506 11.106645 4 >>END_MODULE