FastQCFastQC Report
Thu 26 May 2016
SRR1926863_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926863_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences640784
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT18740.29245424355164923No Hit
CTGTAGGACGTGGAATATGGCAAGA16910.2638954780394017No Hit
CTTTAGGACGTGAAATATGGCGAGG13520.21099153536917278No Hit
GTCCTAAAGTGTGTATTTCTCATTT13370.20865065295013607No Hit
GTCCTACAGTGTGCATTTCTCATTT12310.19210841718894353No Hit
GTATCAACGCAGAGTACTTTTTTTT12090.1886751229743564No Hit
CTGTAGGACCTGGAATATGGCGAGA10460.1632375340208245No Hit
GGTATCAACGCAGAGTACTTTTTTT7300.11392294439311842No Hit
CTGAAGGACCTGGAATATGGCGAGA7070.1103335913505955No Hit
GTACATGGGAAGCAGTGGTATCAAC6530.10190641464206349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA453.4496472E-516.9363161
GGTCTAC456.612511E-414.8192751
TAGGGGT456.651904E-414.8076744
CATAACT456.651904E-414.8076744
CCAACGA653.3979595E-614.60151619
GTAGGGG400.00520164714.2799463
CCAACAA400.005316199314.23314514
TAGGACC3950.014.2185814
TCCAACG954.945832E-913.98671418
GGACCTG5600.013.5988846
AGGACCT6300.013.4477865
CTAGGAC852.62271E-713.439953
TCTACTC500.001474352213.32795053
TATGACG500.001508623813.28737916
CTAACAC655.3365315E-513.1814893
GTAACTC604.014438E-412.6932853
GTAGGAC9500.012.6264793
TGTAGGA9600.012.5047432
GACTGTC701.0994656E-412.2017437
CTGTAGG9400.012.1617151