##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926863_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 640784 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.790228844665286 31.0 30.0 33.0 25.0 34.0 2 30.115341519139054 31.0 30.0 33.0 26.0 34.0 3 30.18268870633474 31.0 30.0 34.0 26.0 34.0 4 33.7954973906964 35.0 35.0 37.0 28.0 37.0 5 33.803877749756545 37.0 35.0 37.0 30.0 37.0 6 33.77804377138006 37.0 35.0 37.0 30.0 37.0 7 33.75853797847637 37.0 35.0 37.0 29.0 37.0 8 33.785440959824214 37.0 35.0 37.0 29.0 37.0 9 35.32200242203301 39.0 35.0 39.0 30.0 39.0 10 35.199741254463284 39.0 35.0 39.0 29.0 39.0 11 35.25309308596969 39.0 35.0 39.0 30.0 39.0 12 35.15189829958301 39.0 35.0 39.0 29.0 39.0 13 35.16737933531424 39.0 35.0 39.0 29.0 39.0 14 36.26290450448201 40.0 36.0 41.0 29.0 41.0 15 36.24659635696272 40.0 36.0 41.0 29.0 41.0 16 36.23727184199356 40.0 36.0 41.0 29.0 41.0 17 36.2019323204075 40.0 36.0 41.0 29.0 41.0 18 36.18171333866014 40.0 36.0 41.0 29.0 41.0 19 36.25019507353492 40.0 37.0 41.0 29.0 41.0 20 36.2088878623686 40.0 37.0 41.0 29.0 41.0 21 36.14994600364553 40.0 37.0 41.0 29.0 41.0 22 36.06269351294664 40.0 37.0 41.0 28.0 41.0 23 35.99132000799021 40.0 36.0 41.0 28.0 41.0 24 35.93128729805988 40.0 36.0 41.0 28.0 41.0 25 35.81178993233289 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.129961741535233 1101 2 0.11686657153524749 1101 3 0.4846467906187044 1101 4 0.49580538426869225 1101 5 0.3893883166904857 1101 6 0.21248335790968298 1101 7 0.2972416384985337 1101 8 0.36899272173253905 1101 9 0.34622385001596 1101 10 0.6965330871974302 1101 11 0.40750436698206016 1101 12 0.4185625309875931 1101 13 0.44747179172193086 1101 14 0.4940782876411447 1101 15 0.5280905402172209 1101 16 0.5268105156301317 1101 17 0.43681645007571035 1101 18 0.7423577759131135 1101 19 0.6520244019057913 1101 20 0.6542129361360267 1101 21 0.6794018089168432 1101 22 0.33542410743357465 1101 23 0.753164966097934 1101 24 0.6368437044310085 1101 25 0.7471108762300034 1102 1 -0.44724033100362703 1102 2 -0.27380700359428545 1102 3 -0.14644129228285152 1102 4 -0.05859876080902637 1102 5 -0.024083185900181547 1102 6 -0.12119539856699646 1102 7 -0.045763542848611394 1102 8 0.014588576654816166 1102 9 -0.08279169402548803 1102 10 -0.15528038430515778 1102 11 -0.19974951902830185 1102 12 -0.046722443105672085 1102 13 -0.1421655139775524 1102 14 -0.06447093515678404 1102 15 -0.1786452110781127 1102 16 -0.06593559835950913 1102 17 -0.04297629603827602 1102 18 -0.052460876936628154 1102 19 -0.2505662716693422 1102 20 -0.29086478407122485 1102 21 -0.20453601491735185 1102 22 -0.369239105001661 1102 23 -0.33802674374662445 1102 24 -0.10927028520629989 1102 25 -0.3285367921637814 1103 1 -0.24415063423984407 1103 2 -0.34072601319251916 1103 3 -0.038044961695913315 1103 4 -0.07309697706656237 1103 5 -0.05220473520792268 1103 6 -0.2357732461844293 1103 7 -0.12634623205272533 1103 8 0.0027203187766247083 1103 9 0.0561724612450476 1103 10 0.17631309229383874 1103 11 0.2250583039564944 1103 12 -0.053071716867670204 1103 13 -0.14150298106325465 1103 14 -0.12089984027867473 1103 15 -0.14157628217299134 1103 16 -0.07915652760438974 1103 17 -0.04782531497992437 1103 18 0.13544152686868927 1103 19 0.09486076476996885 1103 20 0.08391649512694244 1103 21 -0.11187908805079161 1103 22 -0.12394372574403434 1103 23 -0.13462276854913569 1103 24 -0.08795774804499956 1103 25 -0.07374510780125831 1104 1 0.09213530364630884 1104 2 0.10368147506845915 1104 3 0.409780229835512 1104 4 0.33313191328684155 1104 5 0.24321704582840908 1104 6 0.38873247916362175 1104 7 0.3503569560187785 1104 8 0.3136746807504238 1104 9 0.20920195296594812 1104 10 0.37738813966182505 1104 11 0.28863541080219335 1104 12 0.39723308583436534 1104 13 0.38852420404259647 1104 14 0.28361144565921137 1104 15 0.5859393698576554 1104 16 0.4534697183796297 1104 17 0.4457419063727386 1104 18 0.48383719372757383 1104 19 0.5558388107375123 1104 20 0.580637806602347 1104 21 0.4217768967914495 1104 22 0.694374298804938 1104 23 0.7299192036067197 1104 24 0.6413949581087195 1104 25 0.5875253840020207 1105 1 -0.06474293213400628 1105 2 -0.0427398343038341 1105 3 0.013513325181062186 1105 4 0.11772389438304032 1105 5 0.015306554927889238 1105 6 0.062317356121575074 1105 7 -0.22243231830439925 1105 8 -0.30759919620245313 1105 9 -0.15504612901813886 1105 10 -0.013869865804458925 1105 11 -0.019039188227260695 1105 12 0.10783959973586121 1105 13 0.0577989328835784 1105 14 -0.03914664347465191 1105 15 -0.25123844657060346 1105 16 -0.002867467042946714 1105 17 -0.0027092292245214367 1105 18 -0.02804005325405967 1105 19 -0.08475061469439282 1105 20 0.08336232728852622 1105 21 0.04170397922683833 1105 22 -0.05636730020930969 1105 23 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0.5197598301983817 1108 23 0.5200369506506917 1108 24 0.5421692326278276 1108 25 0.6457358068142156 1109 1 0.5674669963227892 1109 2 0.583373188768924 1109 3 0.6081218892567648 1109 4 0.5953855077498744 1109 5 0.5511496708302488 1109 6 0.5532893790098754 1109 7 0.3986846158231785 1109 8 0.25382803795679365 1109 9 0.5257111853448464 1109 10 0.5534944778041506 1109 11 0.5069528042209015 1109 12 0.4603050438649916 1109 13 0.6057687847172346 1109 14 0.5690199151817978 1109 15 0.6587668503687141 1109 16 0.5547512973399265 1109 17 0.5746818160874909 1109 18 0.47102435141238175 1109 19 0.5878709165254961 1109 20 0.5274608760060673 1109 21 0.4360152439524754 1109 22 0.5359133724723932 1109 23 0.4357484475782911 1109 24 0.3711758272638832 1109 25 0.34197308683682337 1110 1 0.578540703426274 1110 2 0.37462990558395504 1110 3 0.5380616765166515 1110 4 0.8010603126802565 1110 5 0.605477063867319 1110 6 0.7689273390647102 1110 7 0.38414621791009296 1110 8 0.3634690173248174 1110 9 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0.6171830453970131 2214 12 0.5921152177746691 2214 13 0.5356913012991242 2214 14 0.595327275278116 2214 15 0.6167998871332969 2214 16 0.5820996855412375 2214 17 0.4831265698028844 2214 18 0.4304270166652344 2214 19 0.508573292941378 2214 20 0.48591087628219753 2214 21 0.559889304403903 2214 22 0.37469208358571393 2214 23 0.40590932369870814 2214 24 0.3736693242899065 2214 25 0.47095287929392526 2215 1 0.1028021876848193 2215 2 0.08089674991597917 2215 3 -0.08589566874134036 2215 4 -0.04857720482279149 2215 5 -0.11352371906475867 2215 6 -0.13027956579399103 2215 7 -0.2019781930087987 2215 8 -0.21801238051007488 2215 9 -0.3260074912014588 2215 10 -0.28481505186834255 2215 11 -0.45349413003174277 2215 12 -0.42079790883362733 2215 13 -0.23440437012595083 2215 14 -0.4544078492888133 2215 15 -0.32577569401131257 2215 16 -0.27048114279648416 2215 17 -0.33121444024585145 2215 18 -0.24151379787164728 2215 19 -0.465230275149942 2215 20 -0.520888910954902 2215 21 -0.4027531964227151 2215 22 -0.4229441637367799 2215 23 -0.5015981158148151 2215 24 -0.512333608387884 2215 25 -0.3678181274483592 2216 1 -0.8587791857268314 2216 2 -0.5721525235852489 2216 3 -2.4184690122210846 2216 4 -1.955294643171463 2216 5 -1.311169633153213 2216 6 -0.928836675018502 2216 7 -0.7795219295826428 2216 8 -0.9983287423881109 2216 9 -1.089612994043243 2216 10 -1.0420235605839494 2216 11 -0.8299982559740542 2216 12 -0.8865795390762727 2216 13 -1.204404092685067 2216 14 -1.2323035394428317 2216 15 -1.2407955508518143 2216 16 -1.103997785286154 2216 17 -1.5940510354913044 2216 18 -1.2702259569166117 2216 19 -1.1851715369510885 2216 20 -1.1487495246541428 2216 21 -1.136081971798646 2216 22 -1.406227359538704 2216 23 -1.4705713070984103 2216 24 -1.403483640491757 2216 25 -1.7356314176594552 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15672.0 3 12.0 4 275.0 5 1633.0 6 2218.0 7 1581.0 8 1311.0 9 1133.0 10 976.0 11 888.0 12 850.0 13 834.0 14 743.0 15 790.0 16 747.0 17 877.0 18 987.0 19 1159.0 20 1428.0 21 1908.0 22 2496.0 23 2975.0 24 3489.0 25 4119.0 26 5059.0 27 5550.0 28 6531.0 29 8126.0 30 10121.0 31 13330.0 32 16993.0 33 22480.0 34 30491.0 35 43073.0 36 63539.0 37 110326.0 38 256064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.503768326063316 19.596545388643776 10.57724755964954 28.322438725643366 2 13.902639812921477 20.75984247663471 40.87547000042177 24.46204771002204 3 18.921639781799453 24.632781588743473 29.01099587167061 27.43458275778647 4 11.997490574046793 17.464075257809185 38.1381245474294 32.40030962071462 5 12.683378280520389 38.84076834719205 35.677766124803355 12.798087247484204 6 31.64760929410991 38.25661840469278 17.70941969752489 12.38635260367242 7 28.192933192020064 31.629873707771868 21.6772781691295 18.499914931078564 8 24.55031409731164 39.307659292208044 19.592336805961995 16.54968980451832 9 26.13738165768207 15.730437035487869 19.968285975596434 38.16389533123363 10 14.71766075592193 29.951588285051674 34.21297408990034 21.11777686912606 11 34.24455715957862 22.587592664845886 24.775809598127193 18.392040577448302 12 23.603889010268738 24.86719310839914 31.94029151971035 19.588626361621774 13 29.26460054215091 22.412493504020876 25.537742223942345 22.785163729885873 14 20.120976803415815 23.190497890084647 27.336980948338287 29.351544358161252 15 22.20484729310049 32.66101721366481 22.865926931660347 22.268208561574358 16 21.283502242586355 28.71384473249706 28.89206558277594 21.110587442140645 17 20.863537266125974 29.818518905776216 28.146658073013796 21.171285755084014 18 20.801178561786323 28.152364068222997 30.674598653830248 20.371858716160435 19 23.320099503419257 27.16009500891099 27.30273322284334 22.217072264826417 20 23.258325439271385 28.152539807140393 28.205288267213085 20.383846486375134 21 22.13199189643924 27.09339384990932 27.287398588432136 23.487215665219306 22 22.62665734475268 29.077817173961897 27.242721416265077 21.05280406502035 23 21.373103489174166 28.803087477488443 28.051193697700622 21.772615335636768 24 22.95566517838332 27.219979306567122 28.813498485446708 21.010857029602846 25 22.077234972984613 28.18589581519042 28.34508525999382 21.391783951831147 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 170.0 1 170.0 2 170.5 3 171.0 4 171.0 5 171.0 6 321.0 7 471.0 8 471.0 9 471.0 10 773.0 11 1075.0 12 1075.0 13 1075.0 14 1802.5 15 2530.0 16 2530.0 17 2530.0 18 4728.0 19 6926.0 20 6926.0 21 6926.0 22 11185.5 23 15445.0 24 15445.0 25 15445.0 26 22840.0 27 30235.0 28 30235.0 29 30235.0 30 40826.5 31 51418.0 32 51418.0 33 51418.0 34 58401.0 35 65384.0 36 65384.0 37 65384.0 38 70694.0 39 76004.0 40 76004.0 41 76004.0 42 79297.5 43 82591.0 44 82591.0 45 82591.0 46 84699.5 47 86808.0 48 86808.0 49 86808.0 50 82229.0 51 77650.0 52 77650.0 53 77650.0 54 67549.5 55 57449.0 56 57449.0 57 57449.0 58 48532.5 59 39616.0 60 39616.0 61 39616.0 62 32066.0 63 24516.0 64 24516.0 65 24516.0 66 18842.0 67 13168.0 68 13168.0 69 13168.0 70 9488.5 71 5809.0 72 5809.0 73 5809.0 74 4000.0 75 2191.0 76 2191.0 77 2191.0 78 1498.0 79 805.0 80 805.0 81 805.0 82 537.0 83 269.0 84 269.0 85 269.0 86 167.0 87 65.0 88 65.0 89 65.0 90 39.5 91 14.0 92 14.0 93 14.0 94 9.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11111388549027441 2 0.09753676745986166 3 0.013889235686284302 4 0.0 5 0.004993882493944918 6 0.3411445978676122 7 0.01981947114784389 8 0.009207470848210941 9 0.009051412020275162 10 0.00468176483807336 11 0.005306000149816475 12 6.242353117431147E-4 13 4.6817648380733604E-4 14 0.0 15 0.0021848235911009015 16 9.363529676146721E-4 17 1.5605882793577868E-4 18 4.6817648380733604E-4 19 3.1211765587155736E-4 20 0.0010924117955504508 21 0.012172588578990737 22 0.0 23 3.1211765587155736E-4 24 1.5605882793577868E-4 25 0.00593023546155959 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 640784.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.52348536117955 #Duplication Level Percentage of deduplicated Percentage of total 1 92.28560601334496 76.15729856889855 2 5.859212095680077 9.67045207211802 3 0.9160009302032276 2.2677476806335877 4 0.319979896421433 1.0562342519282348 5 0.16047070145150077 0.6621300791065569 6 0.09999395073826446 0.49511095979734054 7 0.06474199244450342 0.37399144060233025 8 0.0437852052168559 0.28906461933995603 9 0.034153716143574535 0.2536635324783739 >10 0.17895739412909065 2.779643383808737 >50 0.01835989896271513 1.0463689873227335 >100 0.016466907656629774 2.9157137631940233 >500 9.463740029575116E-4 0.5112300393994694 >1k 0.0013249236041405162 1.5213506213720842 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1874 0.29245424355164923 No Hit CTGTAGGACGTGGAATATGGCAAGA 1691 0.2638954780394017 No Hit CTTTAGGACGTGAAATATGGCGAGG 1352 0.21099153536917278 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1337 0.20865065295013607 No Hit GTCCTACAGTGTGCATTTCTCATTT 1231 0.19210841718894353 No Hit GTATCAACGCAGAGTACTTTTTTTT 1209 0.1886751229743564 No Hit CTGTAGGACCTGGAATATGGCGAGA 1046 0.1632375340208245 No Hit GGTATCAACGCAGAGTACTTTTTTT 730 0.11392294439311842 No Hit CTGAAGGACCTGGAATATGGCGAGA 707 0.1103335913505955 No Hit GTACATGGGAAGCAGTGGTATCAAC 653 0.10190641464206349 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 1.5605882793577868E-4 13 0.0 0.0 0.0 0.0 1.5605882793577868E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTAA 45 3.4496472E-5 16.936316 1 GGTCTAC 45 6.612511E-4 14.819275 1 TAGGGGT 45 6.651904E-4 14.807674 4 CATAACT 45 6.651904E-4 14.807674 4 CCAACGA 65 3.3979595E-6 14.601516 19 GTAGGGG 40 0.005201647 14.279946 3 CCAACAA 40 0.0053161993 14.233145 14 TAGGACC 395 0.0 14.218581 4 TCCAACG 95 4.945832E-9 13.986714 18 GGACCTG 560 0.0 13.598884 6 AGGACCT 630 0.0 13.447786 5 CTAGGAC 85 2.62271E-7 13.43995 3 TCTACTC 50 0.0014743522 13.3279505 3 TATGACG 50 0.0015086238 13.287379 16 CTAACAC 65 5.3365315E-5 13.181489 3 GTAACTC 60 4.014438E-4 12.693285 3 GTAGGAC 950 0.0 12.626479 3 TGTAGGA 960 0.0 12.504743 2 GACTGTC 70 1.0994656E-4 12.201743 7 CTGTAGG 940 0.0 12.161715 1 >>END_MODULE