FastQCFastQC Report
Thu 26 May 2016
SRR1926862_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926862_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523120
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13350.2551995717999694No Hit
GTCCTAAAGTGTGTATTTCTCATTT10230.1955574246826732No Hit
CTGTAGGACGTGGAATATGGCAAGA10040.19192537085181222No Hit
GTACATGGGAAGCAGTGGTATCAAC10020.1915430493959321No Hit
GTCCTACAGTGTGCATTTCTCATTT9160.17510322679308762No Hit
CTTTAGGACGTGAAATATGGCGAGG8810.16841260131518582No Hit
CCCATGTACTCTGCGTTGATACCAC8540.1632512616608044No Hit
GTATCAACGCAGAGTACTTTTTTTT8320.15904572564612326No Hit
CTGTAGGACCTGGAATATGGCGAGA5820.11125554366111026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGG555.6603676E-717.3387241
TAGGACC2350.014.9886554
CTAGCAC456.60719E-414.8199913
GATGTCC400.00523367614.2660398
CTAGGAC551.9094457E-413.8576553
CGAAATC500.001517076613.27677313
CCAACGA500.001517076613.27677319
GTGTTAG655.2477142E-513.2041041
TAGGACT1301.2732926E-1113.1812914
GGCGAGG3100.012.54257519
GAACAGT1600.012.49356656
ATAGGAC1001.3829049E-712.385283
TTAGACT701.067248E-412.2397714
GTCCTAC6250.012.2064611
TCTAGAC550.00299897212.1254473
GGACCTG3850.012.1149736
ACGAAAT550.00310413312.06979512
CCTTGCA657.818346E-411.7257072
GTAGGAC6850.011.6829133
AGGACCT4000.011.6606625
Produced by FastQC (version 0.11.5)