FastQCFastQC Report
Thu 26 May 2016
SRR1926862_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926862_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences523120
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13990.2674338583881328No Hit
GTCCTACAGTGGACATTTCTAAATT13740.2626548401896314No Hit
CTGTAGGACGTGGAATATGGCAAGA12840.24545037467502678No Hit
GTACATGGGAAGCAGTGGTATCAAC11780.22518733751338127No Hit
CTTTAGGACGTGAAATATGGCGAGG10490.20052760360911456No Hit
CCCATGTACTCTGCGTTGATACCAC9860.18848447774889127No Hit
GTCCTAAAGTGTGTATTTCTCATTT9750.1863817097415507No Hit
GTCCTACAGTGTGCATTTCTCATTT8910.17032420859458633No Hit
GGTATCAACGCAGAGTACTTTTTTT8380.16019269001376357No Hit
TATCAACGCAGAGTACTTTTTTTTT7730.14776724269766017No Hit
CTGTAGGACCTGGAATATGGCGAGA7050.13476831319773666No Hit
CTGAAGGACCTGGAATATGGCGAGA5500.1051384003670286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC2050.014.8652884
TAAGGCT602.5038657E-514.2846124
GTCCAGA500.001512068413.28260311
TCCAACG802.0216703E-613.04541518
GGCGAGG2500.012.52359819
GTAGGAC7100.012.4750823
GGACATG2100.012.2439526
CTGTAGG6700.012.0931141
TGTAGGA7050.012.0324262
GTCCTAC6950.011.9324181
CCTACAC802.7849897E-511.9049853
GACGTGG3050.011.8228447
TGGCGAG5700.011.81785518
ACTGTTC2750.011.7323358
AGTAGGA657.789724E-411.7308352
ATAGCCC657.8400935E-411.721833
AGGCAGT657.8464096E-411.7207066
GGACCTG3500.011.6997776
AGGACGT6350.011.6976345
GAGTCCA658.083247E-411.6792479
Produced by FastQC (version 0.11.5)