##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926862_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 523120 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.722811209665085 31.0 30.0 33.0 25.0 34.0 2 30.098061630218687 31.0 30.0 33.0 26.0 34.0 3 30.168833154916655 31.0 30.0 34.0 26.0 34.0 4 33.74081090380792 35.0 35.0 37.0 28.0 37.0 5 33.695962685425904 37.0 35.0 37.0 28.0 37.0 6 33.6399965591069 37.0 35.0 37.0 29.0 37.0 7 33.63226219605444 37.0 35.0 37.0 28.0 37.0 8 33.667368863740634 37.0 35.0 37.0 29.0 37.0 9 35.20073023398073 39.0 35.0 39.0 29.0 39.0 10 35.08288346842025 39.0 35.0 39.0 29.0 39.0 11 35.119681908548706 39.0 35.0 39.0 29.0 39.0 12 35.02491206606515 39.0 35.0 39.0 28.0 39.0 13 35.03039073252791 39.0 35.0 39.0 28.0 39.0 14 36.09341068970791 40.0 36.0 41.0 28.0 41.0 15 36.084613473008105 40.0 36.0 41.0 28.0 41.0 16 36.058143447010245 40.0 36.0 41.0 27.0 41.0 17 36.04699495335678 40.0 36.0 41.0 27.0 41.0 18 36.01633850741704 40.0 36.0 41.0 27.0 41.0 19 36.10233980730999 40.0 36.0 41.0 27.0 41.0 20 36.06004549625325 40.0 36.0 41.0 27.0 41.0 21 36.00315797522557 40.0 36.0 41.0 27.0 41.0 22 35.92104106132436 40.0 36.0 41.0 27.0 41.0 23 35.85417877351277 40.0 36.0 41.0 27.0 41.0 24 35.78177855941276 40.0 36.0 41.0 27.0 41.0 25 35.66386679920477 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.47458192697376234 1101 2 -0.5378456134152394 1101 3 -0.18622700013190752 1101 4 -0.1662628029463633 1101 5 -0.2441640719678091 1101 6 -0.41653469623447137 1101 7 -0.19749672652085337 1101 8 -0.3150044070103277 1101 9 -0.3397835196698935 1101 10 -0.23523247322633267 1101 11 -0.24496917069075863 1101 12 -0.37853092272490585 1101 13 -0.2824787459716589 1101 14 -0.4660922904356397 1101 15 -0.536157111030171 1101 16 -0.2687366956399444 1101 17 -0.423637443888488 1101 18 -0.3860221987234951 1101 19 -0.4813945544869114 1101 20 -0.33965606379590696 1101 21 -0.4601704150464272 1101 22 -0.6040169800255342 1101 23 -0.30509111816281376 1101 24 -0.6435338617110986 1101 25 -0.399264712978308 1102 1 -0.6474266604112664 1102 2 -0.6312659807052583 1102 3 -0.48370167123427876 1102 4 -0.6457375748602701 1102 5 -0.8983502708689599 1102 6 -0.8339112441115475 1102 7 -0.8962802673579304 1102 8 -0.8308502997804652 1102 9 -1.1841909998856295 1102 10 -1.0403150546736484 1102 11 -0.9987655649982656 1102 12 -0.774014566053701 1102 13 -0.7759108746071703 1102 14 -0.7432114328655288 1102 15 -0.832625346996366 1102 16 -0.8882454074365711 1102 17 -0.8551796923217054 1102 18 -0.9854274106797405 1102 19 -0.9672490162373393 1102 20 -0.7641129416627379 1102 21 -0.9332839792381691 1102 22 -1.0588399717757966 1102 23 -0.9701212776130532 1102 24 -0.8322429700330929 1102 25 -0.8535450026434503 1103 1 -0.8833222868709321 1103 2 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0.3990290837524384 2214 11 0.31233799010392715 2214 12 0.5768638199827905 2214 13 0.41955552004317553 2214 14 0.6638912155155907 2214 15 0.3965635154464877 2214 16 0.4533088635974636 2214 17 0.3709640985279634 2214 18 0.4537656075680161 2214 19 0.506248282489473 2214 20 0.4185233498480869 2214 21 0.3799428143694499 2214 22 0.5749731681237122 2214 23 0.6445827157376556 2214 24 0.47096455575178453 2214 25 0.5264466857467482 2215 1 0.2962133596993404 2215 2 0.27406333050640797 2215 3 0.22964674505531235 2215 4 0.1587118972362589 2215 5 0.24199663712500552 2215 6 -0.04444417523271227 2215 7 0.10082883269235055 2215 8 0.029230142652920676 2215 9 -0.05462840561715865 2215 10 0.11250043049477654 2215 11 -0.1482627997896202 2215 12 -0.2121744453900547 2215 13 -0.23232113101742868 2215 14 -0.08177352436879914 2215 15 0.02524292851896348 2215 16 -0.13995480914597636 2215 17 -0.09690920463945929 2215 18 -0.013783368447462863 2215 19 -0.12489104740878076 2215 20 -0.18439951783166464 2215 21 -0.2040984588761745 2215 22 -0.03852825734643517 2215 23 -0.1231127306045039 2215 24 -0.08171506656401561 2215 25 -0.018426852819416695 2216 1 -0.4659842183091847 2216 2 -0.32365330172229534 2216 3 -2.295126356715407 2216 4 -1.8948138527702802 2216 5 -0.837373278916111 2216 6 -0.7877666976609845 2216 7 -0.6048298914464851 2216 8 -0.38933758679546315 2216 9 -0.42395071880210367 2216 10 -0.8148437486175055 2216 11 -0.6761013162544529 2216 12 -0.5711130586299262 2216 13 -0.7168606649279283 2216 14 -0.8205018480682185 2216 15 -1.0277139412009078 2216 16 -0.7016044312002236 2216 17 -0.9284578667899552 2216 18 -0.9312054906978773 2216 19 -1.0324800412099506 2216 20 -1.0959275172039327 2216 21 -1.084797070023619 2216 22 -1.0539285659803355 2216 23 -1.1010820241467343 2216 24 -1.2816366003175759 2216 25 -1.2377151639642392 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13648.0 3 11.0 4 224.0 5 1441.0 6 1925.0 7 1474.0 8 1039.0 9 948.0 10 850.0 11 783.0 12 701.0 13 720.0 14 656.0 15 694.0 16 659.0 17 758.0 18 822.0 19 988.0 20 1298.0 21 1621.0 22 2118.0 23 2506.0 24 2912.0 25 3511.0 26 4223.0 27 4566.0 28 5436.0 29 6685.0 30 8467.0 31 10794.0 32 14009.0 33 18344.0 34 24626.0 35 35129.0 36 51844.0 37 89834.0 38 206856.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.22293139909859 19.942776762389595 10.915858874864837 27.91843296364698 2 13.509464144935745 21.23309427405837 41.347942227767106 23.90949935323878 3 18.398764162234297 25.47978571674384 29.665650822298613 26.45579929872325 4 11.523359840954274 18.08246673803334 38.69819544272824 31.69597797828414 5 12.221083701653233 39.423165155153164 35.84791319375411 12.507837949439491 6 30.70833213439607 38.85803623667981 18.132343490729816 12.301288138194305 7 27.377397090033078 32.276542454543716 22.020534194979255 18.325526260443954 8 24.393824977297708 39.18042345743918 20.12598575730058 16.29976580796253 9 26.093057578851177 15.761058664739597 20.249524923815752 37.89635883259347 10 14.394379659720894 30.55878417128656 34.45115656662206 20.595679602370485 11 33.606065356278506 23.25206173939308 24.754636763002242 18.387236141326166 12 23.075202630371617 25.645167456797672 32.12608961614926 19.153540296681452 13 28.93184915860445 22.864778377386408 25.720916273352717 22.48245619065643 14 20.07244991588928 23.70622419330173 27.74927358923383 28.47205230157516 15 22.399369914969107 32.410954609408456 23.443904080259376 21.745771395363064 16 20.885002781404545 29.093682675195033 29.186969645413935 20.834344897986494 17 20.56220370087169 29.80578070041291 28.546604985471784 21.085410613243617 18 20.40415278359226 28.65543021759867 30.918395240853418 20.022021757955645 19 22.881027070322432 27.60289723753104 27.8772134065098 21.63886228563673 20 22.783534912706287 28.502692504000045 28.732468138224764 19.981304445068904 21 22.0092766435834 27.49247658852006 27.69590504632586 22.80234172157068 22 22.038920324208593 29.366875669062548 27.61526991894785 20.978934087781006 23 21.069544272824594 29.15851047560789 28.360987918641996 21.410957332925523 24 22.39371463526533 27.71696742621196 29.22331396237957 20.66600397614314 25 21.747001886904272 28.324975768478566 28.623783405565888 21.30423893905127 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 206.0 1 206.0 2 215.5 3 225.0 4 225.0 5 225.0 6 355.0 7 485.0 8 485.0 9 485.0 10 752.0 11 1019.0 12 1019.0 13 1019.0 14 1686.0 15 2353.0 16 2353.0 17 2353.0 18 4410.5 19 6468.0 20 6468.0 21 6468.0 22 10246.0 23 14024.0 24 14024.0 25 14024.0 26 20602.5 27 27181.0 28 27181.0 29 27181.0 30 36213.0 31 45245.0 32 45245.0 33 45245.0 34 50576.5 35 55908.0 36 55908.0 37 55908.0 38 59213.0 39 62518.0 40 62518.0 41 62518.0 42 65326.0 43 68134.0 44 68134.0 45 68134.0 46 70111.5 47 72089.0 48 72089.0 49 72089.0 50 67065.5 51 62042.0 52 62042.0 53 62042.0 54 52949.5 55 43857.0 56 43857.0 57 43857.0 58 36397.0 59 28937.0 60 28937.0 61 28937.0 62 23116.0 63 17295.0 64 17295.0 65 17295.0 66 13107.0 67 8919.0 68 8919.0 69 8919.0 70 6381.5 71 3844.0 72 3844.0 73 3844.0 74 2672.0 75 1500.0 76 1500.0 77 1500.0 78 1044.5 79 589.0 80 589.0 81 589.0 82 391.0 83 193.0 84 193.0 85 193.0 86 131.0 87 69.0 88 69.0 89 69.0 90 42.5 91 16.0 92 16.0 93 16.0 94 9.5 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11565224040373145 2 0.09863893561706683 3 0.014145893867563849 4 0.0 5 0.004587857470561248 6 0.3488683284905949 7 0.017204465514604677 8 0.008602232757302338 9 0.008793393485242391 10 0.00382321455880104 11 0.003440893102920936 12 0.0 13 1.91160727940052E-4 14 0.0 15 0.003058571647040832 16 5.73482183820156E-4 17 0.0 18 1.91160727940052E-4 19 1.91160727940052E-4 20 1.91160727940052E-4 21 0.016439822602844472 22 0.0 23 0.0 24 0.0 25 0.007837589845542132 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 523120.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 80.43839378116935 #Duplication Level Percentage of deduplicated Percentage of total 1 91.48067478916028 73.58558542057565 2 6.3655439623335655 10.24068263747065 3 1.0592875365519994 2.556221639779637 4 0.3682185004271362 1.1847561893947864 5 0.18645537748726926 0.7499085538468773 6 0.11053006210868038 0.5334516396333083 7 0.06901961836874554 0.3886279068679829 8 0.05287909169881637 0.3402807360687964 9 0.03770010408148703 0.27292822359279517 >10 0.22468361008682416 3.409742916153571 >50 0.02500408762124953 1.4159470453530438 >100 0.01643124934647948 2.7273060376996208 >500 0.0023813404849960754 1.3908532897691255 >1k 0.0011906702424980377 1.2037077637941311 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1399 0.2674338583881328 No Hit GTCCTACAGTGGACATTTCTAAATT 1374 0.2626548401896314 No Hit CTGTAGGACGTGGAATATGGCAAGA 1284 0.24545037467502678 No Hit GTACATGGGAAGCAGTGGTATCAAC 1178 0.22518733751338127 No Hit CTTTAGGACGTGAAATATGGCGAGG 1049 0.20052760360911456 No Hit CCCATGTACTCTGCGTTGATACCAC 986 0.18848447774889127 No Hit GTCCTAAAGTGTGTATTTCTCATTT 975 0.1863817097415507 No Hit GTCCTACAGTGTGCATTTCTCATTT 891 0.17032420859458633 No Hit GGTATCAACGCAGAGTACTTTTTTT 838 0.16019269001376357 No Hit TATCAACGCAGAGTACTTTTTTTTT 773 0.14776724269766017 No Hit CTGTAGGACCTGGAATATGGCGAGA 705 0.13476831319773666 No Hit CTGAAGGACCTGGAATATGGCGAGA 550 0.1051384003670286 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.91160727940052E-4 0.0 8 0.0 0.0 0.0 1.91160727940052E-4 0.0 9 0.0 0.0 0.0 1.91160727940052E-4 0.0 10 0.0 0.0 0.0 1.91160727940052E-4 0.0 11 0.0 0.0 0.0 1.91160727940052E-4 0.0 12 0.0 0.0 0.0 1.91160727940052E-4 0.0 13 0.0 0.0 0.0 1.91160727940052E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 205 0.0 14.865288 4 TAAGGCT 60 2.5038657E-5 14.284612 4 GTCCAGA 50 0.0015120684 13.282603 11 TCCAACG 80 2.0216703E-6 13.045415 18 GGCGAGG 250 0.0 12.523598 19 GTAGGAC 710 0.0 12.475082 3 GGACATG 210 0.0 12.243952 6 CTGTAGG 670 0.0 12.093114 1 TGTAGGA 705 0.0 12.032426 2 GTCCTAC 695 0.0 11.932418 1 CCTACAC 80 2.7849897E-5 11.904985 3 GACGTGG 305 0.0 11.822844 7 TGGCGAG 570 0.0 11.817855 18 ACTGTTC 275 0.0 11.732335 8 AGTAGGA 65 7.789724E-4 11.730835 2 ATAGCCC 65 7.8400935E-4 11.72183 3 AGGCAGT 65 7.8464096E-4 11.720706 6 GGACCTG 350 0.0 11.699777 6 AGGACGT 635 0.0 11.697634 5 GAGTCCA 65 8.083247E-4 11.679247 9 >>END_MODULE