FastQCFastQC Report
Thu 26 May 2016
SRR1926861_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926861_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554600
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11080.1997836278398846No Hit
CTGTAGGACGTGGAATATGGCAAGA9350.1685899747565813No Hit
GTCCTAAAGTGTGTATTTCTCATTT8140.14677244861161198No Hit
GTCCTACAGTGTGCATTTCTCATTT7940.143166245943022No Hit
GTATCAACGCAGAGTACTTTTTTTT6540.11792282726289216No Hit
CTTTAGGACGTGAAATATGGCGAGG6200.11179228272628922No Hit
CTGTAGGACCTGGAATATGGCGAGA5670.10223584565452579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACCT350.002129820416.3288824
GTCCTAG653.2104872E-614.6807211
TAGGACC2600.013.9214194
GTCTAGG500.001447907313.3594551
TAGGACT500.001467836613.3352544
ACGTTTT500.001519726213.27393419
GTCTTAG801.8953833E-613.1208941
TGTAGGA6450.013.005022
AGGACTG1102.6448106E-912.9818285
TCGAACT604.152809E-412.64184219
GTAGGAC6350.012.6093763
CTGTAGG6300.012.26888751
TCCTATA550.002987712912.1317492
TAACCTG550.00301623912.1163735
TATCCAG550.00301623912.1163735
CCTACAC1052.591496E-711.8016333
TCTTGTA657.7856285E-411.7318012
TTAGCAC657.7856285E-411.7318013
GTCCTAA2850.011.7188211
TGCGTTG1406.9667294E-1011.51310512
Produced by FastQC (version 0.11.5)