##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926861_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 554600 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.03981968986657 31.0 28.0 31.0 16.0 34.0 2 28.016597547782187 31.0 28.0 31.0 16.0 34.0 3 27.683079697078977 31.0 28.0 31.0 16.0 34.0 4 31.455908763072486 35.0 32.0 37.0 17.0 37.0 5 31.710838442120448 35.0 32.0 37.0 19.0 37.0 6 31.166238730616662 35.0 32.0 37.0 13.0 37.0 7 30.935140641904074 35.0 32.0 37.0 11.0 37.0 8 30.866361341507393 35.0 32.0 37.0 11.0 37.0 9 32.88207356653444 37.0 33.0 39.0 17.0 39.0 10 33.090876307248465 37.0 33.0 39.0 17.0 39.0 11 33.250681572304366 37.0 33.0 39.0 17.0 39.0 12 33.13655247024883 38.0 33.0 39.0 17.0 39.0 13 33.23525964659214 38.0 34.0 39.0 17.0 39.0 14 34.215526505589615 39.0 34.0 40.0 17.0 41.0 15 33.82218355571583 39.0 33.0 40.0 10.0 41.0 16 33.885465200144246 39.0 33.0 40.0 12.0 41.0 17 34.100375045077534 39.0 34.0 41.0 17.0 41.0 18 34.135497655968265 39.0 34.0 41.0 16.0 41.0 19 33.50816624594302 39.0 33.0 40.0 10.0 41.0 20 33.817042913811754 39.0 34.0 41.0 10.0 41.0 21 33.79247024882798 39.0 34.0 41.0 9.0 41.0 22 33.80151460512081 39.0 34.0 41.0 2.0 41.0 23 33.792753335737466 39.0 34.0 41.0 2.0 41.0 24 32.51191128741435 38.0 32.0 40.0 2.0 41.0 25 33.071173818968624 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7800115899010542 1101 2 -0.7675837652504676 1101 3 -1.5788193384182634 1101 4 -0.900179787164312 1101 5 -0.7911947369510663 1101 6 -2.5419459810514056 1101 7 -0.9381856703105456 1101 8 -0.5474301316380519 1101 9 -0.737391490277524 1101 10 -9.946658749800342 1101 11 -4.676792791311573 1101 12 -2.2813186136614867 1101 13 -1.359677412628571 1101 14 -1.0449395106905115 1101 15 -2.2865962694167052 1101 16 -0.6751410069270705 1101 17 -0.6935378592703145 1101 18 -0.5286431708070225 1101 19 -0.9556121670097966 1101 20 -0.6395788675241647 1101 21 -0.46445292909948677 1101 22 -0.44584583665374566 1101 23 -0.4968624749318806 1101 24 -0.5829861581387874 1101 25 -0.4354306825533243 1102 1 -0.26957020050215874 1102 2 -0.5306873730900925 1102 3 -0.9464144602966691 1102 4 -0.5491281847580929 1102 5 -0.48583596812447283 1102 6 -1.2110696208346639 1102 7 -0.47289078875575896 1102 8 0.04641997210413251 1102 9 0.11224205352556282 1102 10 0.06626349580740509 1102 11 -0.24827936453564092 1102 12 -0.1919510677984846 1102 13 -0.23292088582726223 1102 14 -0.5749516015059655 1102 15 -0.025485407087806777 1102 16 -0.01079846367628079 1102 17 -0.2655767290029729 1102 18 -0.5805918818663898 1102 19 -0.21385108749402804 1102 20 -0.3863240498566469 1102 21 -0.39246928901768285 1102 22 -0.1578709736083468 1102 23 -0.8006449596690928 1102 24 -3.887116440407084 1102 25 -1.9732510518456365 1103 1 0.05904846388292739 1103 2 -0.4143803224764717 1103 3 -1.7861990024314878E-4 1103 4 -0.10037904257829311 1103 5 -0.060334867440889894 1103 6 0.41617742247077416 1103 7 0.18180204476919215 1103 8 0.3780153839915137 1103 9 0.10467986713125299 1103 10 0.3302448510296401 1103 11 -0.04815993813884489 1103 12 -0.24000682388344075 1103 13 -0.2123242885883343 1103 14 0.0058751143572450815 1103 15 0.33498623805537164 1103 16 0.16793561651765287 1103 17 -0.021091287437243977 1103 18 -0.26382673298445525 1103 19 -1.8382372447268978 1103 20 -0.575441096637924 1103 21 -0.22030811685651486 1103 22 -0.018868451394148167 1103 23 -0.03285354589228007 1103 24 1.4689970324947055 1103 25 0.7118999690718084 1104 1 -0.673571346861884 1104 2 -0.5549480880442381 1104 3 -1.8905937603153866 1104 4 -0.9594869485611994 1104 5 -0.414463377819402 1104 6 -1.875794494501143 1104 7 -0.5089221615800135 1104 8 -0.05524926802313601 1104 9 -0.2728002915989052 1104 10 0.039016981676766704 1104 11 0.00213004965114294 1104 12 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23 0.09368631667611993 1105 24 -4.3407358426365015 1105 25 -2.141953008443476 1106 1 -0.050781243119924824 1106 2 -0.8137253825989177 1106 3 -0.4596116093685687 1106 4 -0.7596463555558515 1106 5 0.013140930416533081 1106 6 0.25366776879504016 1106 7 -2.399558487121375 1106 8 -0.2518012069558182 1106 9 -0.5983105072372084 1106 10 0.005242801645962913 1106 11 -0.14236686980160584 1106 12 -0.17284744223642434 1106 13 -0.29395447622558635 1106 14 -0.6930596375810367 1106 15 0.21122072206370746 1106 16 0.07936772582961993 1106 17 -0.24920710565906745 1106 18 -0.22708883405574198 1106 19 0.3589997546302399 1106 20 0.17902548115020522 1106 21 -0.32768719243346567 1106 22 -0.6026148973314065 1106 23 -0.689208576805612 1106 24 -3.6349520219843825 1106 25 -1.735616393638903 1107 1 0.246038544865943 1107 2 0.09911325108777191 1107 3 0.543921959536025 1107 4 0.32987072336052137 1107 5 0.4430584804754254 1107 6 0.127637144531203 1107 7 -1.9861309252839199 1107 8 -1.2694690514787865 1107 9 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3 -2.807260426982051 2212 4 -1.5693496854619795 2212 5 -0.5250054625462681 2212 6 0.22941732266719583 2212 7 0.5819188361903329 2212 8 0.4406049683202298 2212 9 0.49667434608225847 2212 10 0.6169677899264912 2212 11 0.3643792135307393 2212 12 0.5114397971284035 2212 13 0.41957038678460634 2212 14 0.4970361272573882 2212 15 -0.5513013873626917 2212 16 0.4188779109382068 2212 17 0.38677620738677376 2212 18 0.5445291623279616 2212 19 1.2959667155640204 2212 20 -0.08067016708439212 2212 21 0.031165047660131506 2212 22 0.4830692244565711 2212 23 0.8063459020028247 2212 24 1.968598879922645 2212 25 1.466584897669783 2213 1 -0.34826644343198154 2213 2 -0.23164638974254004 2213 3 0.4184305487089226 2213 4 -7.301150493349741 2213 5 -1.4712295283355523 2213 6 -3.259036252742902 2213 7 -0.8349867220195755 2213 8 -2.432590676614545 2213 9 -0.7832377707863785 2213 10 -0.12835210756732351 2213 11 0.16781852783962137 2213 12 0.2054088715322564 2213 13 0.0619569856929445 2213 14 -0.11683550835337542 2213 15 -2.10632245325332 2213 16 -0.5194880709060357 2213 17 0.010460494114539642 2213 18 0.07455710539938565 2213 19 0.8691109626213063 2213 20 -1.114513135709963 2213 21 0.10320170665330863 2213 22 0.08166989914420242 2213 23 0.30225578921705676 2213 24 0.09845091194858924 2213 25 0.06926482643666532 2214 1 0.8700289993753927 2214 2 0.6918189735275959 2214 3 0.8946139820507071 2214 4 1.0320161592312296 2214 5 1.0237301396176086 2214 6 0.6875085256890792 2214 7 1.2507740464960726 2214 8 -2.60096406028077 2214 9 -0.8710034035628595 2214 10 -0.25118877921093485 2214 11 0.07103335350564066 2214 12 -1.0206689190791565 2214 13 -0.19237906148475048 2214 14 0.1196586554726764 2214 15 -1.6981635478059118 2214 16 -0.21192686855090415 2214 17 0.11245737648018661 2214 18 -0.03361635526864859 2214 19 0.7927383114113482 2214 20 -0.8202770030790418 2214 21 -0.24315620152672324 2214 22 0.18113605121339305 2214 23 -0.056652475331119945 2214 24 -1.0543953379692468 2214 25 0.06078054137971378 2215 1 0.6155628999154352 2215 2 0.1625407484205006 2215 3 0.5983058942469199 2215 4 0.8576535540085182 2215 5 0.7159999088938882 2215 6 1.2814112515600264 2215 7 1.0526286746319684 2215 8 -0.5145418579633194 2215 9 0.06828520423056972 2215 10 -0.3325194261924693 2215 11 -0.13425991580508878 2215 12 -0.5612291089611432 2215 13 -0.13983720405305178 2215 14 -0.05959355352106144 2215 15 -0.2188374482984372 2215 16 0.13346138927234108 2215 17 -0.15358529944932542 2215 18 -0.858134700786394 2215 19 0.461897693962257 2215 20 -3.0684327101114697 2215 21 -1.5439844405328387 2215 22 -0.928852710775999 2215 23 -0.6413628411485632 2215 24 -0.8523523009028189 2215 25 -0.6262705618698092 2216 1 -0.5598076255613975 2216 2 -1.2236886611965758 2216 3 -0.32544376869480374 2216 4 -0.4116745009325733 2216 5 -0.7413183719538061 2216 6 -1.8209721382082016 2216 7 -9.899023447821005 2216 8 -10.921393268358315 2216 9 -5.865764720960392 2216 10 -3.410232536506573 2216 11 -2.15430325016451 2216 12 -1.8407035128154199 2216 13 -1.684767966914361 2216 14 -1.56003501037641 2216 15 -2.6891464919597112 2216 16 -1.7104311147902536 2216 17 -1.8477929082565083 2216 18 -1.520440489598208 2216 19 -0.944883321961214 2216 20 -1.980681825408066 2216 21 -4.783536827144047 2216 22 -3.1818122613967823 2216 23 -2.857591988277779 2216 24 -2.7782078323572144 2216 25 -2.3593490433536743 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34356.0 3 1.0 4 241.0 5 1423.0 6 1897.0 7 1581.0 8 1260.0 9 1128.0 10 1065.0 11 1063.0 12 1053.0 13 970.0 14 1032.0 15 1099.0 16 1220.0 17 1166.0 18 1417.0 19 1718.0 20 2380.0 21 2976.0 22 3623.0 23 4344.0 24 5137.0 25 6015.0 26 6702.0 27 7288.0 28 8164.0 29 9776.0 30 11735.0 31 14140.0 32 18411.0 33 24102.0 34 32361.0 35 45007.0 36 64795.0 37 101419.0 38 132535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.512257164691704 18.35530511478502 12.534664949152052 25.597772771371226 2 14.589444535705068 19.47581904402593 42.03376001184182 23.900976408427184 3 19.38005906606301 23.780589072056266 28.86041490659908 27.978936955281647 4 11.88981151685089 16.70672339565583 38.07103393274078 33.3324311547525 5 12.818117383533552 37.64349210872306 36.51070475929396 13.027685748449427 6 32.842534312045736 36.69450105506159 17.958771439392574 12.504193193500099 7 28.965956230989846 30.659283825864296 21.926639348709028 18.44812059443683 8 24.758343609531007 38.27537075677415 20.16045333400837 16.80583229968647 9 26.224050525617187 15.536050179353678 19.885985525463894 38.35391376956524 10 14.682672102021122 29.476275940439578 34.4537739337476 21.3872780237917 11 34.7349437786855 22.553989594322864 23.989504152352097 18.721562474639544 12 23.292918472350717 24.71636558406778 31.743249311540687 20.247466632040815 13 29.166989776504177 21.840842405957 25.490390563148885 23.501777254389935 14 20.556717763751127 22.346618575293057 27.23282236248873 29.863841298467086 15 23.074550907382594 31.285808835596306 22.913867633771982 22.72577262324912 16 22.075399559231432 28.203417218829692 28.056073552803856 21.66510966913502 17 21.70386895368906 28.79830330880032 27.728673684514266 21.76915405299635 18 21.592976849789633 27.937689018573188 29.472560409299035 20.99677372233814 19 23.885959153639163 26.97288211402642 26.754835265515776 22.386323466818645 20 23.54612001103699 27.589048853108842 27.825839180954336 21.038991954899828 21 22.86297041701833 26.982297820770846 26.726034610862243 23.428697151348583 22 22.973545679644655 28.29596954389787 27.009231690360558 21.72125308609692 23 22.319106651421645 27.933373970690607 27.39108166305379 22.35643771483396 24 23.531565260359745 27.255266888126645 27.711351807567873 21.501816043945738 25 22.711747032493786 27.350650849585033 27.814667469783917 22.12293464813726 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 168.0 1 168.0 2 207.0 3 246.0 4 246.0 5 246.0 6 372.0 7 498.0 8 498.0 9 498.0 10 739.0 11 980.0 12 980.0 13 980.0 14 1613.5 15 2247.0 16 2247.0 17 2247.0 18 3984.0 19 5721.0 20 5721.0 21 5721.0 22 9218.0 23 12715.0 24 12715.0 25 12715.0 26 18644.5 27 24574.0 28 24574.0 29 24574.0 30 32534.0 31 40494.0 32 40494.0 33 40494.0 34 46616.5 35 52739.0 36 52739.0 37 52739.0 38 57194.0 39 61649.0 40 61649.0 41 61649.0 42 65303.5 43 68958.0 44 68958.0 45 68958.0 46 71737.5 47 74517.0 48 74517.0 49 74517.0 50 71634.5 51 68752.0 52 68752.0 53 68752.0 54 61053.5 55 53355.0 56 53355.0 57 53355.0 58 46014.5 59 38674.0 60 38674.0 61 38674.0 62 31712.0 63 24750.0 64 24750.0 65 24750.0 66 19120.0 67 13490.0 68 13490.0 69 13490.0 70 9881.5 71 6273.0 72 6273.0 73 6273.0 74 4353.5 75 2434.0 76 2434.0 77 2434.0 78 1698.5 79 963.0 80 963.0 81 963.0 82 619.0 83 275.0 84 275.0 85 275.0 86 184.0 87 93.0 88 93.0 89 93.0 90 60.5 91 28.0 92 28.0 93 28.0 94 16.0 95 4.0 96 4.0 97 4.0 98 3.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19599711503786513 2 0.11377569419401369 3 0.11611972592859719 4 0.06959971150378652 5 0.02289938694554634 6 0.023440317345834835 7 0.289578074287775 8 0.27803822574828707 9 0.01965380454381536 10 0.03696357735304724 11 0.01712946267580238 12 0.01821132347637937 13 0.01676884240894338 14 0.018031013342949875 15 0.01676884240894338 16 0.020555355210962856 17 0.01965380454381536 18 0.016408222142084386 19 0.023800937612693834 20 0.01821132347637937 21 0.016588532275513882 22 0.01749008294266138 23 0.01839163360980887 24 0.017670393076090876 25 0.01694915254237288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 554600.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.79460390274343 #Duplication Level Percentage of deduplicated Percentage of total 1 94.00307220310788 81.5895941750974 2 4.500682240652056 7.81269864739014 3 0.7505160012461037 1.9542221715247943 4 0.265835229296464 0.922922537207263 5 0.13359763744848327 0.5797777012341715 6 0.07575242992714369 0.39449412901180586 7 0.05168487416204974 0.31401777264607716 8 0.031067966611019213 0.21572254848536573 9 0.024729887745466465 0.19317787302843642 >10 0.1374822075710586 2.2180804176748956 >50 0.012269776982182306 0.7510602301762435 >100 0.011645852093853245 1.9629319063459498 >500 0.0014557315117118325 0.8913064600887005 >1k 2.0796164453026176E-4 0.19999343008875659 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1108 0.1997836278398846 No Hit CTGTAGGACGTGGAATATGGCAAGA 935 0.1685899747565813 No Hit GTCCTAAAGTGTGTATTTCTCATTT 814 0.14677244861161198 No Hit GTCCTACAGTGTGCATTTCTCATTT 794 0.143166245943022 No Hit GTATCAACGCAGAGTACTTTTTTTT 654 0.11792282726289216 No Hit CTTTAGGACGTGAAATATGGCGAGG 620 0.11179228272628922 No Hit CTGTAGGACCTGGAATATGGCGAGA 567 0.10223584565452579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAACCT 35 0.0021298204 16.328882 4 GTCCTAG 65 3.2104872E-6 14.680721 1 TAGGACC 260 0.0 13.921419 4 GTCTAGG 50 0.0014479073 13.359455 1 TAGGACT 50 0.0014678366 13.335254 4 ACGTTTT 50 0.0015197262 13.273934 19 GTCTTAG 80 1.8953833E-6 13.120894 1 TGTAGGA 645 0.0 13.00502 2 AGGACTG 110 2.6448106E-9 12.981828 5 TCGAACT 60 4.152809E-4 12.641842 19 GTAGGAC 635 0.0 12.609376 3 CTGTAGG 630 0.0 12.2688875 1 TCCTATA 55 0.0029877129 12.131749 2 TAACCTG 55 0.003016239 12.116373 5 TATCCAG 55 0.003016239 12.116373 5 CCTACAC 105 2.591496E-7 11.801633 3 TCTTGTA 65 7.7856285E-4 11.731801 2 TTAGCAC 65 7.7856285E-4 11.731801 3 GTCCTAA 285 0.0 11.718821 1 TGCGTTG 140 6.9667294E-10 11.513105 12 >>END_MODULE