FastQCFastQC Report
Thu 26 May 2016
SRR1926861_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926861_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554600
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11310.20393076090876308No Hit
CTGTAGGACGTGGAATATGGCAAGA10860.19581680490443565No Hit
GTATCAACGCAGAGTACTTTTTTTT10610.19130905156869815No Hit
GTCCTACAGTGTGCATTTCTCATTT9140.16480346195456183No Hit
CTTTAGGACGTGAAATATGGCGAGG7800.140641904075009No Hit
GGTATCAACGCAGAGTACTTTTTTT7590.13685539127298954No Hit
GTCCTAAAGTGTGTATTTCTCATTT7330.13216732780382257No Hit
CTGTAGGACCTGGAATATGGCGAGA6460.11648034619545619No Hit
GTACATGGGAAGCAGTGGTATCAAC6300.1135953840605842No Hit
TATCAACGCAGAGTACTTTTTTTTT6060.10926794085827624No Hit
CCCATGTACTCTGCGTTGATACCAC5670.10223584565452579No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGCG359.726123E-519.050155
TAAGACT504.4387216E-617.1451344
TCCAACG755.9249942E-815.1782918
CTCCTAA704.225367E-714.9883261
TAGGACC2650.014.3774724
CTTAGCC602.4973122E-514.2889053
GTAAGAC707.0411697E-613.6084813
TCAATAC500.001466961313.336313
GTCCTAA2800.012.9444641
TGTAGGA6350.012.9164712
CTGGACT905.214479E-712.70014
CGCCACC604.134199E-412.64857614
AATCCCG1001.4628858E-712.3323619
GTCTATT550.002973809612.1393061
AAGGCGT550.003004241312.1228226
CCAACGT550.003097143712.0736419
GATATAC1900.012.0480331
TTAAAGG951.0021831E-612.0327633
GTGTAGA1353.45608E-1012.0108471
CTGTAGG6450.011.9779861
Produced by FastQC (version 0.11.5)