##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926861_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 554600 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.873342949873784 31.0 30.0 33.0 26.0 34.0 2 30.159105661738188 31.0 30.0 34.0 26.0 34.0 3 30.196328885683375 31.0 30.0 34.0 26.0 34.0 4 33.80028489001082 35.0 35.0 37.0 28.0 37.0 5 33.85324738550307 37.0 35.0 37.0 30.0 37.0 6 33.806327082582044 37.0 35.0 37.0 30.0 37.0 7 33.817463036422645 37.0 35.0 37.0 29.0 37.0 8 33.85670393076091 37.0 35.0 37.0 30.0 37.0 9 35.3943058059863 39.0 35.0 39.0 30.0 39.0 10 35.28046159394158 39.0 35.0 39.0 30.0 39.0 11 35.32770645510278 39.0 35.0 39.0 30.0 39.0 12 35.22877028489001 39.0 35.0 39.0 29.0 39.0 13 35.23974035340786 39.0 35.0 39.0 29.0 39.0 14 36.338250991705735 40.0 36.0 41.0 29.0 41.0 15 36.32403534078615 40.0 36.0 41.0 29.0 41.0 16 36.301979805265056 40.0 36.0 41.0 29.0 41.0 17 36.28126397403534 40.0 36.0 41.0 29.0 41.0 18 36.25648034619546 40.0 36.0 41.0 29.0 41.0 19 36.31903173458348 40.0 37.0 41.0 29.0 41.0 20 36.27537504507753 40.0 37.0 41.0 29.0 41.0 21 36.226182834475296 40.0 37.0 41.0 29.0 41.0 22 36.141155787955284 40.0 37.0 41.0 29.0 41.0 23 36.06723584565452 40.0 37.0 41.0 29.0 41.0 24 35.997601875225385 40.0 36.0 41.0 29.0 41.0 25 35.881464118283446 40.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.18369633866563717 1101 2 -0.057570063141181294 1101 3 0.16033679741964235 1101 4 -0.02531793877398769 1101 5 0.008280026575242516 1101 6 0.19339551262267918 1101 7 0.40369069670666136 1101 8 0.39188025862844 1101 9 0.15815161258124988 1101 10 0.18897484601973247 1101 11 0.17992384598756672 1101 12 0.30729919989290977 1101 13 0.22388656039105115 1101 14 0.27087246616772376 1101 15 0.2108623924248434 1101 16 0.2540848872464636 1101 17 0.35177903410018985 1101 18 0.1450520052797728 1101 19 0.14129554507859865 1101 20 0.19232537018268658 1101 21 0.047980066441411395 1101 22 0.038178491299802886 1101 23 0.006288182118282748 1101 24 0.1395213917499447 1101 25 0.16153033389143445 1102 1 -0.6051672386104094 1102 2 -0.5288745277867406 1102 3 -0.3791061294533158 1102 4 -0.6167662990504397 1102 5 -0.4514844264273066 1102 6 -0.29899175171837555 1102 7 -0.30232933523697625 1102 8 -0.2888222028227574 1102 9 -0.31942067109017813 1102 10 -0.24048345559387485 1102 11 -0.1916522888324863 1102 12 -0.025608715859483766 1102 13 -0.13753926021707485 1102 14 -0.019675800069045124 1102 15 -0.32269185951192725 1102 16 -0.32453377440474895 1102 17 -0.20804363393975223 1102 18 -0.25210739943415206 1102 19 -0.20772450179197222 1102 20 -0.3051027193192013 1102 21 -0.1485479290208005 1102 22 -0.31085051173035794 1102 23 -0.16915552095517228 1102 24 -0.2883949577194045 1102 25 -0.13181374683341573 1103 1 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1107 9 0.09535669687384996 1107 10 0.2506439334340129 1107 11 0.15673525168319458 1107 12 0.07557945743155159 1107 13 -0.054109205107153 1107 14 0.10525864406015728 1107 15 -0.13236059817398171 1107 16 0.044891827204700974 1107 17 -0.19860358854499793 1107 18 -0.010393231628313515 1107 19 -0.02485156411518119 1107 20 -0.16929674750177526 1107 21 -0.215281656119231 1107 22 -0.11656870316210188 1107 23 -0.11154803082079212 1107 24 -0.18277477768793915 1107 25 -0.2326107342724839 1108 1 0.7760696764577766 1108 2 0.5742319057559513 1108 3 0.6922315042251768 1108 4 0.739520126570568 1108 5 0.7035147248203586 1108 6 0.7743913750852229 1108 7 0.557564287803821 1108 8 0.5792251009739928 1108 9 0.4989491585935184 1108 10 0.5815558133474497 1108 11 0.6763356023008811 1108 12 0.5218733188827755 1108 13 0.49961062944512236 1108 14 0.5771215744570668 1108 15 0.5410664158233303 1108 16 0.6176888250407302 1108 17 0.405460015695283 1108 18 0.5149977892717885 1108 19 0.3115195427958213 1108 20 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2214 5 0.6573158649101529 2214 6 0.45797105525472404 2214 7 0.605846586144132 2214 8 0.533419723382309 2214 9 0.36369970089286596 2214 10 0.36025227705123086 2214 11 0.4613605732941721 2214 12 0.5049157443481462 2214 13 0.7691535845982287 2214 14 0.6054119442295516 2214 15 0.33152699192180535 2214 16 0.4618900054876889 2214 17 0.401880839990298 2214 18 0.34605010224156985 2214 19 0.27009796180404066 2214 20 0.3234393794716439 2214 21 0.2121537086968459 2214 22 0.4700816625865514 2214 23 0.5428584547983775 2214 24 0.36357552852915376 2214 25 0.4977892003194455 2215 1 0.6703155818552631 2215 2 0.3052209783252984 2215 3 0.641268667678144 2215 4 0.5251883809835647 2215 5 0.33361482925501207 2215 6 0.1918701697565126 2215 7 -0.009100680738427513 2215 8 0.15362842250536346 2215 9 0.14949680371947238 2215 10 3.5914506756284936E-4 2215 11 0.15243407653608898 2215 12 0.07060227275322717 2215 13 0.45029582264923107 2215 14 0.15846343237499383 2215 15 0.2939485574978562 2215 16 0.3343219537500701 2215 17 0.350202622486087 2215 18 0.15106467839274984 2215 19 -0.11228901065689456 2215 20 0.09111786307673952 2215 21 0.013320513561744463 2215 22 -0.1706120529183366 2215 23 0.08076119967653028 2215 24 0.26935561990629253 2215 25 0.060978657022218385 2216 1 -0.30076064223300847 2216 2 -0.07725008238814368 2216 3 -1.7281591326497292 2216 4 -1.8576501627337834 2216 5 -0.6368206858296901 2216 6 -0.16848748188392193 2216 7 -0.32489505252145534 2216 8 -0.35171678482425506 2216 9 -0.24647567800779058 2216 10 -0.32122069777379636 2216 11 -0.31305234926217196 2216 12 -0.36232134089327417 2216 13 -0.41499898701128046 2216 14 -0.6020748586381401 2216 15 -0.9042798880728995 2216 16 -0.5469309246679401 2216 17 -0.6497687424746488 2216 18 -0.47506909019318044 2216 19 -0.7863626074803136 2216 20 -0.496633021626657 2216 21 -0.5650356920092037 2216 22 -0.4980632719358198 2216 23 -0.5080467952264343 2216 24 -0.46504842527025403 2216 25 -0.6956545305110424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12968.0 3 10.0 4 170.0 5 1321.0 6 1820.0 7 1414.0 8 1063.0 9 924.0 10 793.0 11 749.0 12 709.0 13 647.0 14 683.0 15 697.0 16 677.0 17 748.0 18 813.0 19 1047.0 20 1262.0 21 1611.0 22 2116.0 23 2572.0 24 3061.0 25 3652.0 26 4243.0 27 4882.0 28 5757.0 29 6808.0 30 8874.0 31 11381.0 32 14665.0 33 19653.0 34 26710.0 35 37225.0 36 55004.0 37 95240.0 38 222631.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.08698352067903 19.134172252137763 10.446109605806845 28.332734621376364 2 14.32892822721251 20.373826377934282 40.13146732989925 25.165778064953958 3 19.055906221821463 24.198557258791702 28.814607754733995 27.93092876465284 4 12.150558961413632 17.174540209159755 37.35485034258925 33.32005048683736 5 13.128934361639399 38.493136111106104 35.1835389284291 13.194390598825395 6 32.49138692259811 37.516466177385965 17.262344204629347 12.729802695386574 7 28.738405824545836 31.392238868419298 21.107666448444583 18.761688858590286 8 25.244255619953044 38.48942009802684 19.130381467950127 17.135942814069992 9 26.2138460955857 15.756330934343662 19.489341597254654 38.540481372815975 10 14.866392702132991 29.816092741622835 33.65021376807021 21.667300788173964 11 34.866779186682244 22.701273784278932 23.73649399171968 18.695453037319137 12 23.40938842188396 24.872069498988456 31.308262921972315 20.410279157155273 13 29.288430573161122 21.953114998259977 25.282235016435532 23.47621941214336 14 20.660656328885683 22.533717994951317 26.837179949513164 29.96844572664984 15 23.04644956543691 31.280969382235206 22.77903999422987 22.893541058098023 16 22.070800959976346 28.41739136706473 27.761239242188473 21.75056843077045 17 21.57284529390552 28.993689145329967 27.500901550667145 21.932564010097366 18 21.527279415211847 28.02915285776622 29.301975859818175 21.141591867203758 19 23.487908315242088 27.09395668198112 26.935462931575415 22.482672071201378 20 23.357891927759507 27.704871328832724 27.841187756044082 21.096048987363684 21 22.809833298468597 26.939140596259133 26.854743888452077 23.39628221682019 22 22.921384781824738 28.27353047241255 26.908041831950957 21.897042913811756 23 22.221240533717996 27.968806346916697 27.374864767399927 22.43508835196538 24 23.08320621423085 26.99504866588051 27.99433102896152 21.927414090927122 25 22.65282533706234 27.502159326758886 27.655791593862645 22.189223742316134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 178.0 1 178.0 2 171.5 3 165.0 4 165.0 5 165.0 6 280.5 7 396.0 8 396.0 9 396.0 10 681.0 11 966.0 12 966.0 13 966.0 14 1583.5 15 2201.0 16 2201.0 17 2201.0 18 3901.5 19 5602.0 20 5602.0 21 5602.0 22 8873.5 23 12145.0 24 12145.0 25 12145.0 26 17850.0 27 23555.0 28 23555.0 29 23555.0 30 31717.5 31 39880.0 32 39880.0 33 39880.0 34 45704.0 35 51528.0 36 51528.0 37 51528.0 38 56391.5 39 61255.0 40 61255.0 41 61255.0 42 64823.0 43 68391.0 44 68391.0 45 68391.0 46 71777.0 47 75163.0 48 75163.0 49 75163.0 50 72689.0 51 70215.0 52 70215.0 53 70215.0 54 62254.0 55 54293.0 56 54293.0 57 54293.0 58 46989.0 59 39685.0 60 39685.0 61 39685.0 62 32534.0 63 25383.0 64 25383.0 65 25383.0 66 19516.5 67 13650.0 68 13650.0 69 13650.0 70 9938.0 71 6226.0 72 6226.0 73 6226.0 74 4298.0 75 2370.0 76 2370.0 77 2370.0 78 1657.0 79 944.0 80 944.0 81 944.0 82 620.0 83 296.0 84 296.0 85 296.0 86 191.0 87 86.0 88 86.0 89 86.0 90 54.5 91 23.0 92 23.0 93 23.0 94 13.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11413631446087269 2 0.09844933285250632 3 0.018031013342949875 4 0.0 5 0.005589614136314461 6 0.3519653804543815 7 0.02145690587811035 8 0.010457987738910927 9 0.010998918139199423 10 0.004868373602596465 11 0.00576992426974396 12 3.606202668589975E-4 13 0.0016227912008654885 14 0.0 15 0.003606202668589975 16 5.409304002884963E-4 17 0.0 18 0.0010818608005769925 19 7.21240533717995E-4 20 0.00144248106743599 21 0.013703570140641903 22 0.0 23 0.0 24 3.606202668589975E-4 25 0.005228993869455464 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 554600.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.21405518716617 #Duplication Level Percentage of deduplicated Percentage of total 1 92.52493758268096 77.91900199777001 2 5.67297421711759 9.55488327591423 3 0.9131381291905056 2.3069719441546477 4 0.32477731834122264 1.0940326004131025 5 0.1540521318651774 0.6486677367297325 6 0.09264120911260304 0.46810151380887327 7 0.05535694127622174 0.3263282755339918 8 0.03959855057018701 0.26678036184396153 9 0.02859664792666482 0.2167415717997701 >10 0.16323380033645662 2.499360372370037 >50 0.015668710049211304 0.9133644276527547 >100 0.012663727578037125 2.1746824774531786 >500 0.0017171156037859132 1.018563358024421 >1k 6.439183514197175E-4 0.5925200865313315 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1131 0.20393076090876308 No Hit CTGTAGGACGTGGAATATGGCAAGA 1086 0.19581680490443565 No Hit GTATCAACGCAGAGTACTTTTTTTT 1061 0.19130905156869815 No Hit GTCCTACAGTGTGCATTTCTCATTT 914 0.16480346195456183 No Hit CTTTAGGACGTGAAATATGGCGAGG 780 0.140641904075009 No Hit GGTATCAACGCAGAGTACTTTTTTT 759 0.13685539127298954 No Hit GTCCTAAAGTGTGTATTTCTCATTT 733 0.13216732780382257 No Hit CTGTAGGACCTGGAATATGGCGAGA 646 0.11648034619545619 No Hit GTACATGGGAAGCAGTGGTATCAAC 630 0.1135953840605842 No Hit TATCAACGCAGAGTACTTTTTTTTT 606 0.10926794085827624 No Hit CCCATGTACTCTGCGTTGATACCAC 567 0.10223584565452579 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.8031013342949875E-4 0.0 10 0.0 0.0 0.0 1.8031013342949875E-4 0.0 11 0.0 0.0 0.0 1.8031013342949875E-4 0.0 12 0.0 0.0 0.0 1.8031013342949875E-4 0.0 13 0.0 0.0 0.0 1.8031013342949875E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAGGCG 35 9.726123E-5 19.05015 5 TAAGACT 50 4.4387216E-6 17.145134 4 TCCAACG 75 5.9249942E-8 15.17829 18 CTCCTAA 70 4.225367E-7 14.988326 1 TAGGACC 265 0.0 14.377472 4 CTTAGCC 60 2.4973122E-5 14.288905 3 GTAAGAC 70 7.0411697E-6 13.608481 3 TCAATAC 50 0.0014669613 13.33631 3 GTCCTAA 280 0.0 12.944464 1 TGTAGGA 635 0.0 12.916471 2 CTGGACT 90 5.214479E-7 12.7001 4 CGCCACC 60 4.134199E-4 12.648576 14 AATCCCG 100 1.4628858E-7 12.33236 19 GTCTATT 55 0.0029738096 12.139306 1 AAGGCGT 55 0.0030042413 12.122822 6 CCAACGT 55 0.0030971437 12.07364 19 GATATAC 190 0.0 12.048033 1 TTAAAGG 95 1.0021831E-6 12.032763 3 GTGTAGA 135 3.45608E-10 12.010847 1 CTGTAGG 645 0.0 11.977986 1 >>END_MODULE