FastQCFastQC Report
Thu 26 May 2016
SRR1926860_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926860_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567079
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT13110.23118472029470322No Hit
GTCCTAAAGTGTGTATTTCTCATTT12070.21284512387162988No Hit
CTGTAGGACGTGGAATATGGCAAGA10690.18850989015639796No Hit
GTATCAACGCAGAGTACTTTTTTTT8850.15606291186942206No Hit
CTTTAGGACGTGAAATATGGCGAGG8840.1558865695961233No Hit
GTCCTACAGTGTGCATTTCTCATTT8690.15324143549664157No Hit
GTACATGGGAAGCAGTGGTATCAAC6500.11462247764420831No Hit
CTGTAGGACCTGGAATATGGCGAGA6120.1079214712588546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGCG307.622226E-419.0339957
TGCCGGT453.588533E-516.85791810
CTAGACT350.002137104316.3206314
CTAGGAC653.2201333E-614.6766153
TAGGACC2800.014.6205654
ATAGCAC400.005129312614.3096983
AGTCCTG602.5176183E-514.2767595
CGGTTTC551.990374E-413.7904113
CCGGTTT551.990374E-413.7904112
TCCAACG903.7194695E-813.69464318
GCCGGTT500.001520372813.2732711
GGAGTGC604.0237268E-412.6893296
GTCTTAG550.002952078812.1512671
TTAGTCA550.003015525912.1168324
ATACTTA550.003023538312.1125416
TGTAGGA6100.011.5728722
GGCGAGG2550.011.52580819
GTCCTAC7700.011.4073121
ATAGGAC855.0873343E-511.2232933
AGGACGT5050.011.1198535
Produced by FastQC (version 0.11.5)