##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926860_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567079 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.09337499713444 31.0 28.0 31.0 16.0 34.0 2 28.084857665334106 31.0 28.0 31.0 16.0 34.0 3 27.730638940958844 31.0 28.0 31.0 16.0 34.0 4 31.497260522784302 35.0 32.0 37.0 18.0 37.0 5 31.722525432964368 35.0 32.0 37.0 19.0 37.0 6 31.158200180221804 35.0 32.0 37.0 12.0 37.0 7 30.94458091377039 35.0 32.0 37.0 11.0 37.0 8 30.878997458907843 35.0 32.0 37.0 11.0 37.0 9 32.88333547883099 37.0 32.0 39.0 17.0 39.0 10 33.056983242193766 37.0 33.0 39.0 17.0 39.0 11 33.23319854905578 38.0 33.0 39.0 17.0 39.0 12 33.10281283560139 38.0 33.0 39.0 17.0 39.0 13 33.21605455324567 38.0 33.0 39.0 17.0 39.0 14 34.19346863488156 39.0 34.0 40.0 17.0 41.0 15 33.7857882235103 39.0 33.0 40.0 10.0 41.0 16 33.86453033880641 39.0 33.0 40.0 13.0 41.0 17 34.08727179105557 39.0 34.0 41.0 17.0 41.0 18 34.13069783927813 39.0 34.0 41.0 16.0 41.0 19 33.48479488748481 39.0 33.0 40.0 10.0 41.0 20 33.78460496685647 39.0 33.0 41.0 10.0 41.0 21 33.76055893446945 39.0 34.0 41.0 9.0 41.0 22 33.790729334008134 39.0 34.0 41.0 2.0 41.0 23 33.78150486969188 39.0 34.0 41.0 2.0 41.0 24 32.46825574567212 38.0 32.0 40.0 2.0 41.0 25 33.04312979320341 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.5400733814377681 1101 2 -0.7706075944194026 1101 3 -1.7228893564252559 1101 4 -1.0618894547776208 1101 5 -0.8671523531280947 1101 6 -3.0238876538676394 1101 7 -1.2181324004864145 1101 8 -0.7437354059006971 1101 9 -0.9855156263700735 1101 10 -9.015721080164742 1101 11 -4.208260530759254 1101 12 -1.9269916304711465 1101 13 -1.260478946274926 1101 14 -1.1402388302434332 1101 15 -2.191262725248933 1101 16 -0.8097697815712479 1101 17 -0.7523041404243642 1101 18 -0.5587525060960914 1101 19 -1.316385967968671 1101 20 -0.7284291624101087 1101 21 -0.6333700903919564 1101 22 -0.4007653549781409 1101 23 -0.42121286824030335 1101 24 -0.4208209330915125 1101 25 -0.24657130794123816 1102 1 -0.6450853146119933 1102 2 -0.7729176982181762 1102 3 -1.24464118041519 1102 4 -1.1340497277950412 1102 5 -0.6175874462975557 1102 6 -1.3432602126689943 1102 7 -0.9046782425830244 1102 8 -0.16465899390289707 1102 9 -0.14866847871050481 1102 10 -0.2771766439717922 1102 11 -0.6678351315222955 1102 12 -0.6065646439236048 1102 13 -0.6940726741305241 1102 14 -0.8776579766104788 1102 15 -0.2721475375211071 1102 16 -0.3884380279898778 1102 17 -0.40013094216360656 1102 18 -0.6779822069447334 1102 19 -0.38876449619161946 1102 20 -0.5219342276315686 1102 21 -0.5556570648079102 1102 22 -0.5686461252772901 1102 23 -0.752753863370657 1102 24 -3.627451923063113 1102 25 -2.486606682291434 1103 1 0.07572878985294196 1103 2 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-2.647977887560888 2216 16 -1.5006471568681619 2216 17 -2.0315475093871527 2216 18 -1.688463511638453 2216 19 -1.044750971927499 2216 20 -2.0537013318479573 2216 21 -4.541332283582772 2216 22 -3.3234939773059295 2216 23 -3.113374388430323 2216 24 -3.2951702007082986 2216 25 -2.592493714908777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34809.0 3 7.0 4 246.0 5 1519.0 6 2011.0 7 1599.0 8 1361.0 9 1125.0 10 1129.0 11 1057.0 12 1022.0 13 1043.0 14 1083.0 15 1134.0 16 1194.0 17 1250.0 18 1593.0 19 1857.0 20 2451.0 21 3035.0 22 3839.0 23 4648.0 24 5215.0 25 6150.0 26 6892.0 27 7666.0 28 8347.0 29 10197.0 30 11885.0 31 14653.0 32 18944.0 33 24405.0 34 32783.0 35 45395.0 36 66009.0 37 102433.0 38 137093.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.94170029612772 18.6427950499318 12.893747393864007 25.521757260076473 2 14.338506629941557 20.000529689072515 42.22222222222222 23.438741458763708 3 18.80160181935037 24.739209966875958 29.273813643715265 27.185374570058407 4 11.816575993449646 17.4163162380644 38.408680629695056 32.3584271387909 5 12.592050375256857 38.013034774096255 36.44850911464075 12.946405736006138 6 31.833502953507608 37.28757057463317 18.228671512453413 12.650254959405807 7 27.993427336337845 31.225989260168525 22.70027521702525 18.08030818646838 8 24.407532647603954 38.194159509896856 20.799848611327267 16.59845923117192 9 26.314350620489197 15.654917703166998 20.293594745419526 37.73713693092428 10 15.017429101660351 29.24119900929317 34.60646923093207 21.13490265811441 11 33.859091237779786 22.574961507325515 24.965299272827966 18.600647982066736 12 23.453478240931812 24.773449598047204 32.02517892984257 19.747893231178416 13 28.948807218418537 22.488245025520552 25.576284757863398 22.986662998197513 14 20.4340824954276 22.946653286042586 27.37566337265802 29.243600845871786 15 22.806782387239462 31.816337976500797 23.061457006903023 22.315422629356718 16 21.50272501190539 28.243293295941584 28.743496128542954 21.510485563610068 17 21.392087143018397 29.07847951236031 28.063445366294165 21.465987978327124 18 21.264651623803797 27.737291931950857 30.2296993661398 20.768357078105545 19 23.975962479737962 26.868825481041437 26.965307162750662 22.189904876469935 20 23.519493461026304 27.786096633949754 27.99421501450656 20.700194890517388 21 22.55700627337967 27.037261088643895 27.029677301018168 23.376055336958267 22 22.91965395909945 28.599874776228646 27.226469836062684 21.25400142860922 23 21.884838362049962 28.281570504623645 27.90571751587807 21.927873617448327 24 23.465887345257137 27.24056374991402 28.072502288404184 21.22104661642466 25 22.453068451982023 27.550988014024792 28.189264121792746 21.806679412200438 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 261.5 3 294.0 4 294.0 5 294.0 6 465.5 7 637.0 8 637.0 9 637.0 10 879.0 11 1121.0 12 1121.0 13 1121.0 14 1887.0 15 2653.0 16 2653.0 17 2653.0 18 4646.5 19 6640.0 20 6640.0 21 6640.0 22 10503.0 23 14366.0 24 14366.0 25 14366.0 26 20969.0 27 27572.0 28 27572.0 29 27572.0 30 36356.0 31 45140.0 32 45140.0 33 45140.0 34 50550.5 35 55961.0 36 55961.0 37 55961.0 38 60167.5 39 64374.0 40 64374.0 41 64374.0 42 67584.0 43 70794.0 44 70794.0 45 70794.0 46 72814.0 47 74834.0 48 74834.0 49 74834.0 50 71405.5 51 67977.0 52 67977.0 53 67977.0 54 59963.0 55 51949.0 56 51949.0 57 51949.0 58 44483.5 59 37018.0 60 37018.0 61 37018.0 62 30106.5 63 23195.0 64 23195.0 65 23195.0 66 17957.0 67 12719.0 68 12719.0 69 12719.0 70 9323.0 71 5927.0 72 5927.0 73 5927.0 74 4142.5 75 2358.0 76 2358.0 77 2358.0 78 1624.0 79 890.0 80 890.0 81 890.0 82 599.0 83 308.0 84 308.0 85 308.0 86 197.5 87 87.0 88 87.0 89 87.0 90 59.0 91 31.0 92 31.0 93 31.0 94 16.5 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.19521089654175167 2 0.12502667176883644 3 0.12749546359501937 4 0.06912617113312254 5 0.023629864622036787 6 0.02221912643564653 7 0.3010162605210209 8 0.29043572412309393 9 0.018868623242969673 10 0.037208219666043006 11 0.015518120050292818 12 0.017105200509981853 13 0.014989093230396471 14 0.01587080459689038 15 0.014283724137201343 16 0.019221307789567238 17 0.018163254149774547 18 0.013578355044006216 19 0.023277180075439222 20 0.01657617369008551 21 0.01410738186390256 22 0.01657617369008551 23 0.017986911876475765 24 0.01587080459689038 25 0.014636408683798906 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 567079.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.36363468570254 #Duplication Level Percentage of deduplicated Percentage of total 1 93.36318339927226 78.76457497390452 2 4.8439909408293635 8.17313364305962 3 0.8581756132010663 2.1719644178482054 4 0.3385291499544377 1.142381981488704 5 0.15556665004244044 0.6562084016729488 6 0.09621239330097298 0.4870096320408252 7 0.06522581788168251 0.38518809502924994 8 0.044018119051855584 0.29708228121940056 9 0.030768351361926627 0.23361569587610242 >10 0.17043186417097084 2.6809579564575237 >50 0.018204340480693727 1.1032927423988272 >100 0.013600912392040148 2.386991654260457 >500 0.001464713642206582 0.8843978840252962 >1k 6.277344180885352E-4 0.6332006407183108 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1311 0.23118472029470322 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1207 0.21284512387162988 No Hit CTGTAGGACGTGGAATATGGCAAGA 1069 0.18850989015639796 No Hit GTATCAACGCAGAGTACTTTTTTTT 885 0.15606291186942206 No Hit CTTTAGGACGTGAAATATGGCGAGG 884 0.1558865695961233 No Hit GTCCTACAGTGTGCATTTCTCATTT 869 0.15324143549664157 No Hit GTACATGGGAAGCAGTGGTATCAAC 650 0.11462247764420831 No Hit CTGTAGGACCTGGAATATGGCGAGA 612 0.1079214712588546 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 1.76342273298782E-4 0.0 13 0.0 0.0 0.0 1.76342273298782E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACAGCG 30 7.622226E-4 19.033995 7 TGCCGGT 45 3.588533E-5 16.857918 10 CTAGACT 35 0.0021371043 16.320631 4 CTAGGAC 65 3.2201333E-6 14.676615 3 TAGGACC 280 0.0 14.620565 4 ATAGCAC 40 0.0051293126 14.309698 3 AGTCCTG 60 2.5176183E-5 14.276759 5 CGGTTTC 55 1.990374E-4 13.79041 13 CCGGTTT 55 1.990374E-4 13.79041 12 TCCAACG 90 3.7194695E-8 13.694643 18 GCCGGTT 50 0.0015203728 13.27327 11 GGAGTGC 60 4.0237268E-4 12.689329 6 GTCTTAG 55 0.0029520788 12.151267 1 TTAGTCA 55 0.0030155259 12.116832 4 ATACTTA 55 0.0030235383 12.112541 6 TGTAGGA 610 0.0 11.572872 2 GGCGAGG 255 0.0 11.525808 19 GTCCTAC 770 0.0 11.407312 1 ATAGGAC 85 5.0873343E-5 11.223293 3 AGGACGT 505 0.0 11.119853 5 >>END_MODULE