FastQCFastQC Report
Thu 26 May 2016
SRR1926860_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926860_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences567079
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14960.2638080408549779No Hit
CTGTAGGACGTGGAATATGGCAAGA13930.24564478670520334No Hit
GTCCTACAGTGGACATTTCTAAATT13510.2382384112266545No Hit
CTTTAGGACGTGAAATATGGCGAGG12050.2124924393250323No Hit
GTCCTAAAGTGTGTATTTCTCATTT11420.20138287610720904No Hit
GTCCTACAGTGTGCATTTCTCATTT9770.17228640101291No Hit
GGTATCAACGCAGAGTACTTTTTTT9650.17017029373332465No Hit
TATCAACGCAGAGTACTTTTTTTTT8300.14636408683798904No Hit
CTGTAGGACCTGGAATATGGCGAGA7640.13472549680026946No Hit
GTACATGGGAAGCAGTGGTATCAAC7460.13155133588089138No Hit
CCCATGTACTCTGCGTTGATACCAC6080.10721610216565945No Hit
CTGAAGGACCTGGAATATGGCGAGA5870.10351291442638504No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACA551.092337E-515.5949834
ACTGTCC704.4975604E-714.9061168
TCGTCAA400.005109035414.3182422
TTAGGCT801.2345117E-714.29544
CTCCTAG759.1446964E-713.99881651
CCAACGA551.9862951E-413.79374519
TAGGACC2650.013.6660434
TAAGACA852.5865847E-713.4544944
CCTTACA500.001444520313.3636932
ACTAGAC500.001460060713.3447393
TAGACAG500.001462013313.3423745
CTAAGAC853.785899E-612.3354733
GTTCTAA550.002958521712.1477341
GTCCTAC8050.012.093841
AATCCCG1103.8889993E-812.06952719
GGCGAGG3400.011.99345919
GGACTGT657.7986106E-411.7295596
GTCTTAA751.9811503E-411.4535771
TCAAGAC752.0058006E-411.4383483
TAGGGCT752.0091077E-411.436324
Produced by FastQC (version 0.11.5)