##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926860_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 567079 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.85295170514161 31.0 30.0 33.0 26.0 34.0 2 30.09059584290725 31.0 30.0 33.0 26.0 34.0 3 30.18842348244248 31.0 30.0 34.0 26.0 34.0 4 33.79289305370151 35.0 35.0 37.0 28.0 37.0 5 33.786537678171825 37.0 35.0 37.0 30.0 37.0 6 33.77153800440503 37.0 35.0 37.0 29.0 37.0 7 33.760892221365985 37.0 35.0 37.0 29.0 37.0 8 33.7902197048383 37.0 35.0 37.0 29.0 37.0 9 35.329072316202854 39.0 35.0 39.0 30.0 39.0 10 35.192937844638934 39.0 35.0 39.0 29.0 39.0 11 35.2488683234611 39.0 35.0 39.0 30.0 39.0 12 35.14304179840904 39.0 35.0 39.0 29.0 39.0 13 35.16013994522809 39.0 35.0 39.0 29.0 39.0 14 36.23313506583739 40.0 36.0 41.0 29.0 41.0 15 36.22724876075468 40.0 36.0 41.0 29.0 41.0 16 36.20931651498292 40.0 36.0 41.0 29.0 41.0 17 36.18996647733385 40.0 36.0 41.0 29.0 41.0 18 36.16838570992754 40.0 36.0 41.0 29.0 41.0 19 36.224681217255444 40.0 37.0 41.0 29.0 41.0 20 36.19983106410218 40.0 37.0 41.0 29.0 41.0 21 36.14117257031207 40.0 37.0 41.0 29.0 41.0 22 36.058866577672596 40.0 36.0 41.0 28.0 41.0 23 35.990294121277635 40.0 36.0 41.0 28.0 41.0 24 35.92547422845847 40.0 36.0 41.0 28.0 41.0 25 35.8028299408019 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.20506780963970073 1101 2 -0.22595718432203427 1101 3 0.08482522335614817 1101 4 -0.08072423947018592 1101 5 -0.05803003082883862 1101 6 -0.032884381681427044 1101 7 -0.0416366335439875 1101 8 -0.06346167813946835 1101 9 0.007462627761519514 1101 10 -0.019271457803483827 1101 11 0.3089953318919214 1101 12 0.21187990196243334 1101 13 0.22108189953016222 1101 14 0.42824884342871883 1101 15 0.167128015482632 1101 16 0.2481887531366027 1101 17 0.18328286410210382 1101 18 0.09435771591375186 1101 19 0.014834948277375304 1101 20 0.26395960995211 1101 21 0.22295525467298205 1101 22 0.3047860237793927 1101 23 0.29816426088878245 1101 24 0.18412694271005137 1101 25 0.3129058914311784 1102 1 -1.0446190542612683 1102 2 -0.8913606163142802 1102 3 -0.6847539692696607 1102 4 -0.83291954669982 1102 5 -0.8923714953496145 1102 6 -0.9769770352679004 1102 7 -0.9249778089474745 1102 8 -0.9121116137967036 1102 9 -1.0412111175544965 1102 10 -1.0794163565840904 1102 11 -0.875708096132044 1102 12 -0.7684856329895808 1102 13 -1.069602854918564 1102 14 -0.8313784787216321 1102 15 -1.131938305755014 1102 16 -1.0314953720568525 1102 17 -1.1540149663844659 1102 18 -1.1181009714937673 1102 19 -1.3086476111475633 1102 20 -1.114548771614679 1102 21 -0.9960794337322838 1102 22 -0.9930756421030651 1102 23 -1.0847007213390043 1102 24 -1.1267620079075868 1102 25 -1.2031769661813954 1103 1 -0.3799988286105602 1103 2 -0.3682687006439629 1103 3 -0.20522173818352485 1103 4 -0.3718628634025194 1103 5 -0.4365933343539652 1103 6 -0.4619993901769135 1103 7 -0.34082805840695585 1103 8 -0.29964756446332075 1103 9 -0.44617180573446547 1103 10 -0.3691564177901867 1103 11 -0.18291032097089754 1103 12 -0.36625705152587074 1103 13 -0.24264539690059195 1103 14 -0.4536510502703024 1103 15 -0.30875512793200954 1103 16 -0.21468183186264156 1103 17 -0.21434155246022613 1103 18 -0.4519405170831021 1103 19 -0.4459669221614959 1103 20 -0.49251515144372604 1103 21 -0.48789031091415325 1103 22 -0.5715423803591833 1103 23 -0.5465073664904523 1103 24 -0.49434839746742654 1103 25 -0.48601046889190513 1104 1 -0.6203015736162953 1104 2 -0.5763772063369821 1104 3 -0.43223636056135106 1104 4 -0.48117322852064603 1104 5 -0.5452005307998746 1104 6 -0.4808393089741685 1104 7 -0.6938555084643241 1104 8 -0.6932513774616424 1104 9 -0.6368511431671635 1104 10 -0.5396468705837378 1104 11 -0.6566121068428004 1104 12 -0.6173941841275976 1104 13 -0.6694864337066164 1104 14 -0.7286076538773472 1104 15 -0.6784776852299572 1104 16 -0.5006699456641641 1104 17 -0.48087042944983693 1104 18 -0.9004253209902515 1104 19 -0.6070322668318298 1104 20 -0.8312173786348751 1104 21 -0.8234238658878255 1104 22 -0.8992925348209226 1104 23 -0.8769878160476594 1104 24 -0.6687842443590668 1104 25 -0.6579793411741193 1105 1 0.009987706020687881 1105 2 -0.05068236799325021 1105 3 0.08492189241652426 1105 4 0.29721588095184615 1105 5 -0.08907460907553855 1105 6 -0.018729269269122995 1105 7 -0.500265037675895 1105 8 -0.3573466695773533 1105 9 -0.3163179610911513 1105 10 -0.30263609349922405 1105 11 -0.14214812499367468 1105 12 -0.18874982791658823 1105 13 -0.13278762485806084 1105 14 0.028312368811249655 1105 15 -0.059873034067166486 1105 16 0.026896702095335456 1105 17 -0.11793792803180025 1105 18 -0.09089610941906301 1105 19 -0.1690572242907109 1105 20 -0.09202282389863115 1105 21 -0.1346263616337069 1105 22 -0.04668334593833379 1105 23 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0.3051713241994918 2214 14 0.31450857028180224 2214 15 0.3014399254806506 2214 16 0.4084393457377118 2214 17 0.334305024570412 2214 18 0.3704156526100135 2214 19 0.01306142586616943 2214 20 0.23371065633033083 2214 21 0.35093874746024767 2214 22 0.3450645524162752 2214 23 0.31527814059748493 2214 24 0.06742182933916041 2214 25 0.22757867043071656 2215 1 0.4706375647470509 2215 2 0.15555299146966917 2215 3 0.25818736610581894 2215 4 0.16904205400601313 2215 5 0.27492838544255704 2215 6 0.20987441592009048 2215 7 0.2776854078794173 2215 8 0.1404340061211684 2215 9 -0.10535657588503966 2215 10 0.01177019498878451 2215 11 -0.23525228109500773 2215 12 0.0031842926852121423 2215 13 -0.02454304034744581 2215 14 -0.1372518805065326 2215 15 -0.07878921170971154 2215 16 -0.06141381011215685 2215 17 -0.07182476422683237 2215 18 -0.2514662753250292 2215 19 -0.3526405032880291 2215 20 -0.34741295647850734 2215 21 -0.18392965318868448 2215 22 -0.2072655865140547 2215 23 -0.14946204546285458 2215 24 -0.21812699946885772 2215 25 -0.3369462383197259 2216 1 -0.10137372808783951 2216 2 -0.011438181946736847 2216 3 -2.035208921536963 2216 4 -1.6916407081954432 2216 5 -0.40724321372670147 2216 6 0.017026544684370037 2216 7 -0.16811871984644 2216 8 -0.4181751579494488 2216 9 -0.24642939519968365 2216 10 -0.1178734965999979 2216 11 -0.31542816375336713 2216 12 -0.1296655255910082 2216 13 -0.25442037679604823 2216 14 -0.4319104994058023 2216 15 -0.39826935189662294 2216 16 -0.290278685501562 2216 17 -0.21219664896203483 2216 18 -0.21013060554309249 2216 19 -0.321818852197687 2216 20 -0.0062427851389443845 2216 21 -0.31948585775774063 2216 22 -0.1629866405119742 2216 23 -0.2926636290557951 2216 24 -0.48151290289565196 2216 25 -0.34690210540176025 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13661.0 3 15.0 4 199.0 5 1440.0 6 1974.0 7 1469.0 8 1118.0 9 967.0 10 855.0 11 767.0 12 741.0 13 683.0 14 744.0 15 665.0 16 705.0 17 799.0 18 893.0 19 1001.0 20 1307.0 21 1658.0 22 2163.0 23 2612.0 24 3181.0 25 3811.0 26 4435.0 27 4923.0 28 5818.0 29 7214.0 30 9169.0 31 11687.0 32 15357.0 33 20199.0 34 27492.0 35 38497.0 36 56849.0 37 97966.0 38 224045.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.537057371753 19.54925977056948 10.757424361722036 28.156258495955484 2 14.115730313278874 20.867430153003227 40.386815033431944 24.630024500285955 3 18.365950361914972 25.354330986412315 29.397302216641037 26.882416435031676 4 12.250321383793088 17.68483756231495 37.64501947700409 32.41982157688788 5 12.958952618102042 38.741067838575646 35.11776697727898 13.182212566043324 6 31.461465375544286 38.03284895602033 17.738188547982723 12.767497120452658 7 27.90047550513602 31.9188113782594 21.78593097415058 18.394782142454 8 24.88536640424391 38.33470893648241 19.76036287705635 17.019561782217334 9 26.372744572070523 15.622525444383895 19.830306108592097 38.17442387495348 10 15.130647320129581 29.622386304118958 33.77749483741608 21.469471538335384 11 33.8236383323164 22.839477870144712 24.719342858452865 18.617540939086023 12 23.723720546379862 24.954944469720214 31.4484074501215 19.872927533778423 13 29.16323532783026 22.54455744105298 25.436051894187212 22.856155336929554 14 20.609334165670333 23.076366919541933 27.08586825798215 29.22843065680559 15 22.495560458009077 32.04564572190392 23.042765971329466 22.416027848757544 16 21.541204353561074 28.38279172456602 28.39337231693812 21.682631604934787 17 21.176628259251814 29.306021393882325 27.948536180208013 21.568814166657848 18 21.211934181777785 27.73150736143416 30.32745112215801 20.729107334630044 19 23.605259955879006 26.9550695937236 27.31833595790342 22.121334492493965 20 23.56734623340393 27.696767811073276 28.0289978256921 20.706888129830695 21 22.480092417040716 26.82986622456988 27.159851497808624 23.53018986058078 22 23.013372034584247 28.612062869547277 26.973137781508395 21.40142731436008 23 21.755169914597435 28.452296769938577 27.76420921952673 22.02832409593725 24 23.17084568464006 27.060603549064595 28.446653817193017 21.321896949102328 25 22.417757994942207 27.743251870218643 28.06438983786148 21.77460029697767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 203.0 1 203.0 2 219.5 3 236.0 4 236.0 5 236.0 6 376.0 7 516.0 8 516.0 9 516.0 10 820.0 11 1124.0 12 1124.0 13 1124.0 14 1845.5 15 2567.0 16 2567.0 17 2567.0 18 4408.0 19 6249.0 20 6249.0 21 6249.0 22 9913.0 23 13577.0 24 13577.0 25 13577.0 26 20086.0 27 26595.0 28 26595.0 29 26595.0 30 35590.5 31 44586.0 32 44586.0 33 44586.0 34 50123.0 35 55660.0 36 55660.0 37 55660.0 38 59642.5 39 63625.0 40 63625.0 41 63625.0 42 66925.5 43 70226.0 44 70226.0 45 70226.0 46 73282.0 47 76338.0 48 76338.0 49 76338.0 50 72741.5 51 69145.0 52 69145.0 53 69145.0 54 60968.5 55 52792.0 56 52792.0 57 52792.0 58 45031.5 59 37271.0 60 37271.0 61 37271.0 62 30551.0 63 23831.0 64 23831.0 65 23831.0 66 18405.0 67 12979.0 68 12979.0 69 12979.0 70 9468.0 71 5957.0 72 5957.0 73 5957.0 74 4162.5 75 2368.0 76 2368.0 77 2368.0 78 1626.0 79 884.0 80 884.0 81 884.0 82 572.0 83 260.0 84 260.0 85 260.0 86 167.0 87 74.0 88 74.0 89 74.0 90 43.5 91 13.0 92 13.0 93 13.0 94 8.0 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11162465899812901 2 0.09786996168082401 3 0.016752515963384288 4 0.0 5 0.004761241379067114 6 0.3325815274415029 7 0.018163254149774547 8 0.00969882503143301 9 0.010227851851329357 10 0.003174160919378076 11 0.0029978186460792944 12 1.76342273298782E-4 13 3.52684546597564E-4 14 1.76342273298782E-4 15 0.002821476372780512 16 5.290268198963461E-4 17 1.76342273298782E-4 18 3.52684546597564E-4 19 3.52684546597564E-4 20 3.52684546597564E-4 21 0.014812750957097687 22 0.0 23 0.0 24 0.0 25 0.005819295018859806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 567079.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 81.12192217476705 #Duplication Level Percentage of deduplicated Percentage of total 1 91.39448782653726 74.14096528667051 2 6.36746787757551 10.330824672300192 3 1.113482292254962 2.7098347156586473 4 0.3982934735522237 1.2924132865688454 5 0.2034094735960976 0.8250483743336481 6 0.11677252078039861 0.5683686805739318 7 0.08075876415578735 0.45859143265433044 8 0.05302711170353941 0.3441328983013762 9 0.0350603080892107 0.2559743625812675 >10 0.19585861578793953 2.9155842852589506 >50 0.02265107162842817 1.3108073322884835 >100 0.016117083268177908 2.715959805749841 >500 0.0015245889577897608 0.9676887754383177 >1k 0.001088992112706972 1.1638060916217268 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1496 0.2638080408549779 No Hit CTGTAGGACGTGGAATATGGCAAGA 1393 0.24564478670520334 No Hit GTCCTACAGTGGACATTTCTAAATT 1351 0.2382384112266545 No Hit CTTTAGGACGTGAAATATGGCGAGG 1205 0.2124924393250323 No Hit GTCCTAAAGTGTGTATTTCTCATTT 1142 0.20138287610720904 No Hit GTCCTACAGTGTGCATTTCTCATTT 977 0.17228640101291 No Hit GGTATCAACGCAGAGTACTTTTTTT 965 0.17017029373332465 No Hit TATCAACGCAGAGTACTTTTTTTTT 830 0.14636408683798904 No Hit CTGTAGGACCTGGAATATGGCGAGA 764 0.13472549680026946 No Hit GTACATGGGAAGCAGTGGTATCAAC 746 0.13155133588089138 No Hit CCCATGTACTCTGCGTTGATACCAC 608 0.10721610216565945 No Hit CTGAAGGACCTGGAATATGGCGAGA 587 0.10351291442638504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.76342273298782E-4 0.0 8 0.0 0.0 0.0 1.76342273298782E-4 0.0 9 0.0 0.0 0.0 1.76342273298782E-4 0.0 10 0.0 0.0 0.0 1.76342273298782E-4 0.0 11 0.0 0.0 0.0 1.76342273298782E-4 0.0 12 0.0 0.0 0.0 1.76342273298782E-4 0.0 13 0.0 0.0 0.0 1.76342273298782E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTAGACA 55 1.092337E-5 15.594983 4 ACTGTCC 70 4.4975604E-7 14.906116 8 TCGTCAA 40 0.0051090354 14.318242 2 TTAGGCT 80 1.2345117E-7 14.2954 4 CTCCTAG 75 9.1446964E-7 13.9988165 1 CCAACGA 55 1.9862951E-4 13.793745 19 TAGGACC 265 0.0 13.666043 4 TAAGACA 85 2.5865847E-7 13.454494 4 CCTTACA 50 0.0014445203 13.363693 2 ACTAGAC 50 0.0014600607 13.344739 3 TAGACAG 50 0.0014620133 13.342374 5 CTAAGAC 85 3.785899E-6 12.335473 3 GTTCTAA 55 0.0029585217 12.147734 1 GTCCTAC 805 0.0 12.09384 1 AATCCCG 110 3.8889993E-8 12.069527 19 GGCGAGG 340 0.0 11.993459 19 GGACTGT 65 7.7986106E-4 11.729559 6 GTCTTAA 75 1.9811503E-4 11.453577 1 TCAAGAC 75 2.0058006E-4 11.438348 3 TAGGGCT 75 2.0091077E-4 11.43632 4 >>END_MODULE