##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926859_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 30719 Sequences flagged as poor quality 0 Sequence length 25 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.57300042319086 31.0 28.0 33.0 10.0 34.0 2 27.751456753149515 31.0 28.0 33.0 10.0 34.0 3 27.22764412904066 31.0 27.0 33.0 10.0 34.0 4 30.72919040333344 35.0 32.0 37.0 10.0 37.0 5 31.050685243660276 35.0 32.0 37.0 10.0 37.0 6 30.32927504150526 35.0 31.0 37.0 10.0 37.0 7 30.28721638074156 35.0 31.0 37.0 8.0 37.0 8 30.17634037566327 35.0 30.0 37.0 2.0 37.0 9 32.03942185618021 37.0 32.0 39.0 2.0 39.0 10 32.18164653797324 37.0 32.0 39.0 2.0 39.0 11 32.290732120186206 38.0 32.0 39.0 2.0 39.0 12 32.20430352550539 37.0 32.0 39.0 2.0 39.0 13 32.29789381164751 38.0 33.0 39.0 2.0 39.0 14 33.25769068003516 39.0 33.0 41.0 2.0 41.0 15 32.8508414987467 39.0 32.0 41.0 2.0 41.0 16 32.91357140531918 38.0 32.0 41.0 2.0 41.0 17 33.16191933331163 39.0 33.0 41.0 2.0 41.0 18 33.21195351411179 39.0 33.0 41.0 2.0 41.0 19 32.645333506950095 39.0 32.0 41.0 2.0 41.0 20 32.87512614342915 39.0 32.0 41.0 2.0 41.0 21 32.76522673264104 39.0 32.0 41.0 2.0 41.0 22 32.82864025521664 39.0 33.0 41.0 2.0 41.0 23 32.78391223672646 39.0 33.0 41.0 2.0 41.0 24 31.59529932614994 38.0 31.0 40.0 2.0 41.0 25 32.12340896513558 39.0 32.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.6430628210818057 1101 2 -0.5090649412265122 1101 3 -0.45939522405857147 1101 4 -1.0724112930511005 1101 5 -1.2459404017161155 1101 6 -2.995185801757465 1101 7 0.36482772017980025 1101 8 0.4110708572030539 1101 9 -0.11223970734060629 1101 10 -6.938032112170962 1101 11 -2.8124627324478197 1101 12 -1.1769900417439842 1101 13 -0.7295317040071687 1101 14 -1.1480618587338824 1101 15 -2.334563750289142 1101 16 -0.7725832825650372 1101 17 -1.0281026971110307 1101 18 -0.9095472587342854 1101 19 -1.8592084496204961 1101 20 -2.027273176528979 1101 21 -1.50578558849406 1101 22 -0.9616156583828328 1101 23 -1.304362044414674 1101 24 0.08076453828101293 1101 25 -0.9163328824857366 1102 1 0.06288955987057321 1102 2 0.32664934448777316 1102 3 1.1846523949890475 1102 4 -0.20931605495586325 1102 5 -0.1530832588589739 1102 6 0.11552848395681892 1102 7 1.2481610535131331 1102 8 0.37178514291733933 1102 9 1.1865698164689178 1102 10 0.5833964592576066 1102 11 1.050632505647421 1102 12 2.000390910636966 1102 13 1.7561825817071153 1102 14 0.04836671269468695 1102 15 0.2201981544727616 1102 16 0.4488452888635308 1102 17 0.5980877790794423 1102 18 -0.9083567825438124 1102 19 0.49912488371283814 1102 20 1.5393934901376838 1102 21 0.20492869722022533 1102 22 0.9752891035219236 1102 23 0.964685574632945 1102 24 -1.5370926045761308 1102 25 -0.41871383486668634 1103 1 -0.6513961544151385 1103 2 -0.2209697031312743 1103 3 -0.9820142716776203 1103 4 -0.899792245432053 1103 5 -1.2959404017161162 1103 6 0.3060046744330087 1103 7 0.033875339227421364 1103 8 -0.8901196189874199 1103 9 0.2818079117070127 1103 10 -0.48803211217096276 1103 11 -0.28270082768591465 1103 12 -1.5948471846011252 1103 13 -1.3628650373405016 1103 14 -0.9278237634957875 1103 15 -2.636944702670096 1103 16 -1.5511547111364656 1103 17 -1.9257217447300796 1103 18 -2.027404401591429 1103 19 -2.381827497239545 1103 20 -1.5558446051004076 1103 21 -1.5569760646845339 1103 22 -2.1913775631447407 1103 23 -2.9876953777480075 1103 24 -1.2513783188618461 1103 25 -1.9425233586762118 1104 1 0.39027051225152576 1104 2 0.6451017254401563 1104 3 -0.9343952240585729 1104 4 0.26092204028223165 1104 5 0.26655959828388376 1104 6 -0.6076858017574658 1104 7 0.9564943868464688 1104 8 1.9194041905363903 1104 9 1.9544269593260566 1104 10 1.8869678878290372 1104 11 1.958370600885516 1104 12 2.3396766249226815 1104 13 1.8246349626594949 1104 14 1.5811048079327819 1104 15 2.8154362497108565 1104 16 2.0024167174349614 1104 17 2.121897302888968 1104 18 1.9279527412657131 1104 19 1.5824582170461667 1104 20 1.8102268234710195 1104 21 1.835881078172605 1104 22 1.9842176749505 1104 23 2.2414712889186603 1104 24 3.397431204947676 1104 25 2.2420004508475984 1105 1 0.3819371789181929 1105 2 0.6853795032179342 1105 3 0.4544936648303164 1105 4 1.2303664847266766 1105 5 -0.051495957271672665 1105 6 0.44092530935364493 1105 7 0.8592721646242438 1105 8 1.186070857203056 1105 9 0.6405380704371666 1105 10 8.567767179243901E-4 1105 11 0.31253726755218025 1105 12 0.6591210693671314 1105 13 -0.018420592896056576 1105 14 1.0547159190438933 1105 15 0.8154362497108565 1105 16 -0.0781388381205943 1105 17 0.9330084140000778 1105 18 0.3487860745990474 1105 19 -2.189764005176052 1105 20 -0.4383842876400905 1105 21 -0.12523003293850365 1105 22 -0.6199489917161713 1105 23 -0.26547315552578965 1105 24 -4.005346572830099 1105 25 -2.577443993596848 1106 1 0.9958906672902863 1106 2 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1107 15 -0.7999483656737567 1107 16 0.5120321020503482 1107 17 0.5642049951966612 1107 18 -0.30762418181120665 1107 19 0.24271462730258264 1107 20 0.7765729773171728 1107 21 0.9384451807367071 1107 22 -0.5173848891520691 1107 23 -0.3466697367223688 1107 24 -4.201927769411295 1107 25 -2.1009482671011206 1108 1 0.051502396309498266 1108 2 -1.2634127673134685 1108 3 -0.4343952240585729 1108 4 -1.3387156408771865 1108 5 -0.8611577930204639 1108 6 -2.800620584366161 1108 7 -1.6286505406897653 1108 8 0.08607085720305463 1108 9 -1.380717968210174 1108 10 -1.422814720866615 1108 11 -1.1657236020130384 1108 12 0.04800995825601717 1108 13 -0.4643143127028182 1108 14 -0.5448009891686638 1108 15 -1.9236941850717528 1108 16 -0.5791050216954758 1108 17 -1.2911461753718996 1108 18 -0.9062863891690682 1108 19 -0.8559475800552789 1108 20 -2.1272731765289805 1108 21 -1.2764377624071024 1108 22 -0.4822678322958822 1108 23 -1.3934924791972847 1108 24 -0.7203224182407268 1108 25 -0.18372418683356528 1109 1 2.1819371789181936 1109 2 1.6209350587734868 1109 3 0.8056047759414255 1109 4 1.4725887069489012 1109 5 1.2040595982838838 1109 6 2.8698141982425334 1109 7 2.654827720179803 1109 8 3.3460708572030526 1109 9 1.7227602926593946 1109 10 2.0719678878290395 1109 11 1.0725372675521783 1109 12 1.7880099582560192 1109 13 2.110468295992831 1109 14 1.2169381412661195 1109 15 0.3954362497108548 1109 16 1.3574167174349583 1109 17 1.8218973028889707 1109 18 2.315452741265716 1109 19 -1.4142084496204959 1109 20 0.6327268234710175 1109 21 1.9192144115059406 1109 22 1.0733843416171638 1109 23 1.3056379555853255 1109 24 1.465764538281011 1109 25 2.4536671175142644 1110 1 1.160660583173513 1110 2 0.8919988885607211 1110 3 1.735817541898875 1110 4 1.2866312601403855 1110 5 2.06576172594346 1110 6 1.2659844110084926 1110 7 0.24461495422235302 1110 8 0.6711772401817768 1110 9 1.2372283777657742 1110 10 1.001329589956697 1110 11 0.7380691824457983 1110 12 0.7820525114475032 1110 13 1.3640853172694278 1110 14 1.1152360136065411 1110 15 1.7728830582214954 1110 16 0.8774167174349614 1110 17 -0.6121452503025182 1110 18 -0.25688768426620356 1110 19 -0.38739993898219893 1110 20 0.34081192985399866 1110 21 -0.03823239700469827 1110 22 0.6495545543831227 1110 23 0.2286166789895816 1110 24 -1.1708312063998392 1110 25 0.17196498985468622 1111 1 1.4419371789181952 1111 2 -0.11906494122651168 1111 3 1.3656047759414278 1111 4 1.5125887069489004 1111 5 1.3240595982838812 1111 6 1.049814198242533 1111 7 0.9348277201798005 1111 8 1.6060708572030578 1111 9 2.142760292659389 1111 10 1.1319678878290347 1111 11 0.8125372675521803 1111 12 1.1680099582560146 1111 13 0.7904682959928309 1111 14 0.31693814126612097 1111 15 -0.48456375028914067 1111 16 0.4574167174349597 1111 17 0.7018973028889661 1111 18 0.8754527412657112 1111 19 1.5257915503795019 1111 20 1.4127268234710186 1111 21 0.6192144115059435 1111 22 0.9733843416171624 1111 23 1.205637955585324 1111 24 0.9257645382810118 1111 25 0.7536671175142615 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2215 15 1.056176990451597 2215 16 1.2477870878053352 2215 17 1.0848602658519297 2215 18 -0.5326954068824321 2215 19 2.0917174763054263 2215 20 -1.8124583617141674 2215 21 -0.9511559588644332 2215 22 -0.5643934361606142 2215 23 -0.5988064888591182 2215 24 0.9761349086513853 2215 25 0.23737082121796504 2216 1 -0.007717993495599984 2216 2 -0.3383752860540987 2216 3 0.3414668449069431 2216 4 0.3629335345351059 2216 5 -0.020078332750600225 2216 6 -1.7046685603781562 2216 7 -10.809310210854683 2216 8 -12.775998108314187 2216 9 -6.854481086650953 2216 10 -3.5569976294123435 2216 11 -1.7219454910685101 2216 12 -1.6416452141577764 2216 13 -1.3226351522830306 2216 14 -1.3678894449407792 2216 15 -1.9776671985650047 2216 16 -1.1743074204960742 2216 17 -1.9643095936627546 2216 18 -1.7810989828722192 2216 19 -0.5238636220342912 2216 20 -1.661755935149671 2216 21 -4.804923519528543 2216 22 -3.372822554934558 2216 23 -3.326775837518124 2216 24 -3.8942354617189885 2216 25 -3.431850123865047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2553.0 3 1.0 4 18.0 5 85.0 6 154.0 7 111.0 8 101.0 9 104.0 10 70.0 11 72.0 12 58.0 13 62.0 14 65.0 15 76.0 16 87.0 17 86.0 18 85.0 19 95.0 20 162.0 21 193.0 22 186.0 23 225.0 24 275.0 25 323.0 26 388.0 27 381.0 28 424.0 29 492.0 30 597.0 31 707.0 32 898.0 33 1225.0 34 1611.0 35 2255.0 36 3257.0 37 5307.0 38 7930.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.73056217168586 20.411758948089663 13.429475676204769 24.42820320401971 2 15.32870506176461 21.889768912356182 39.291418141520815 23.490107884358398 3 18.044916718276347 25.747253821832523 30.626813129502267 25.581016330388866 4 12.514657980456025 18.32899022801303 38.35830618892508 30.79804560260586 5 11.96600677259703 37.825605626465226 37.399062255795776 12.809325345141964 6 30.06967960406356 38.55170617348268 18.595337327429018 12.783276895024745 7 25.347006760508183 34.13240145008001 23.073908357555766 17.446683431856037 8 23.284537968892955 37.30558096980786 21.967716638347927 17.442164422951247 9 24.230781753654803 16.99606030019861 21.853938071826263 36.91921987432032 10 13.449264035430506 30.884460075550347 35.8961834049759 19.770092484043246 11 31.50680471446246 25.476981181220292 24.327668164355018 18.688545939962232 12 20.723448590219444 27.896073451846064 31.311454060037768 20.069023897896724 13 26.82490069675067 24.649996744155757 26.023963013609432 22.501139545484143 14 19.12219581284798 24.67359098752971 29.420766450688635 26.783446748933677 15 22.42625512795468 31.82262160578238 24.809533111935924 20.941590154327017 16 20.68830788265555 30.817569107544035 27.37277374401719 21.121349265783216 17 19.822225116400222 30.101260052746394 28.82167160485788 21.254843225995508 18 19.35924985348701 30.292374812788957 29.807254020967637 20.541121312756395 19 21.4046169374532 29.26448083873278 29.971022042783186 19.359880181030835 20 21.47554860975451 29.230969590414794 29.488181285407304 19.80530051442339 21 20.814612228950967 29.113759197759983 29.55980985869636 20.511818714592696 22 20.37507325649541 29.071433222634628 29.85609168457381 20.69740183629615 23 20.407631698899525 29.019339714788046 29.478413752686073 21.09461483362636 24 20.567837723439585 29.53798065965552 29.5737961123954 20.320385504509492 25 20.86344989255714 28.57980074233249 29.957022856026565 20.599726509083805 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 107.0 1 107.0 2 100.0 3 93.0 4 93.0 5 93.0 6 104.5 7 116.0 8 116.0 9 116.0 10 157.0 11 198.0 12 198.0 13 198.0 14 272.0 15 346.0 16 346.0 17 346.0 18 492.5 19 639.0 20 639.0 21 639.0 22 896.0 23 1153.0 24 1153.0 25 1153.0 26 1517.5 27 1882.0 28 1882.0 29 1882.0 30 2245.0 31 2608.0 32 2608.0 33 2608.0 34 2938.0 35 3268.0 36 3268.0 37 3268.0 38 3437.0 39 3606.0 40 3606.0 41 3606.0 42 3664.5 43 3723.0 44 3723.0 45 3723.0 46 3663.5 47 3604.0 48 3604.0 49 3604.0 50 3382.0 51 3160.0 52 3160.0 53 3160.0 54 2880.5 55 2601.0 56 2601.0 57 2601.0 58 2198.5 59 1796.0 60 1796.0 61 1796.0 62 1397.5 63 999.0 64 999.0 65 999.0 66 751.5 67 504.0 68 504.0 69 504.0 70 351.0 71 198.0 72 198.0 73 198.0 74 140.5 75 83.0 76 83.0 77 83.0 78 56.5 79 30.0 80 30.0 81 30.0 82 16.5 83 3.0 84 3.0 85 3.0 86 2.5 87 2.0 88 2.0 89 2.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2278720010417006 2 0.12370194342263745 3 0.1302125720238289 4 0.061850971711318724 5 0.02278720010417006 6 0.02278720010417006 7 0.32553143005957225 8 0.3743611445685081 9 0.019531885803574334 10 0.03580845730655295 11 0.016276571502978614 12 0.016276571502978614 13 0.016276571502978614 14 0.019531885803574334 15 0.016276571502978614 16 0.019531885803574334 17 0.019531885803574334 18 0.016276571502978614 19 0.019531885803574334 20 0.016276571502978614 21 0.016276571502978614 22 0.016276571502978614 23 0.016276571502978614 24 0.019531885803574334 25 0.016276571502978614 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 30719.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 90.34148247013249 #Duplication Level Percentage of deduplicated Percentage of total 1 93.29057365234938 84.28008724242325 2 5.5419429230325745 10.013346788632441 3 0.7855289708849812 2.1289755525896026 4 0.21620063418852695 0.7812754321429733 5 0.050446814643989625 0.2278720010417006 6 0.028826751225136928 0.15625508642859468 7 0.021620063418852694 0.13672320062502033 8 0.010810031709426347 0.07812754321429734 9 0.007206687806284232 0.05859565741072301 >10 0.03243009512827904 0.6771053745239103 >50 0.007206687806284232 0.5175949737947199 >100 0.007206687806284232 0.9440411471727596 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 149 0.4850418307887626 No Hit GGTATCAACGCAGAGTACTTTTTTT 141 0.4589993163839969 No Hit TATCAACGCAGAGTACTTTTTTTTT 88 0.2864676584524236 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 71 0.2311273153422963 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 50 0.16276571502978612 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE