FastQCFastQC Report
Thu 26 May 2016
SRR1926859_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926859_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30719
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3000.9765942901787167No Hit
GGTATCAACGCAGAGTACTTTTTTT2570.8366157752531007No Hit
TATCAACGCAGAGTACTTTTTTTTT1480.48178651648816695No Hit
ACGCAGAGTACTTTTTTTTTTTTTT980.3190208014583808No Hit
GTACTTTTTTTTTTTTTTTTTTTTT430.13997851492561608No Hit
GCAGAGTACTTTTTTTTTTTTTTTT350.1139360005208503No Hit
GAGTACTTTTTTTTTTTTTTTTTTT320.10417005761906312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA801.801207E-613.077081
AACGCAG1002.1684973E-510.4616636
TCAACGC1002.1684973E-510.4616634
ATCAACG1002.1684973E-510.4616633
CAACGCA1002.1684973E-510.4616635
TATCAAC1053.7141675E-59.963492
ACGCAGA1201.6286972E-48.7038557
CGCAGAG1252.5351453E-48.3557018
AGAGTAC1303.8710245E-48.034327511
CAGAGTA1355.806831E-47.73675910
GCAGAGT1355.806831E-47.7367599
GAGTACT1250.002369697.596091712
ACTTTTT1408.568475E-47.46044716
AGTACTT1300.00348031477.303933613
GTACTTT1350.00502897947.033417714
TACTTTT1400.0071579666.782224715
Produced by FastQC (version 0.11.5)