FastQCFastQC Report
Thu 26 May 2016
SRR1926858_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926858_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522742
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10420.19933351442968042No Hit
GTCCTACAGTGTGCATTTCTCATTT9160.17522984569826033No Hit
GTCCTAAAGTGTGTATTTCTCATTT8230.15743904258697405No Hit
CTGTAGGACGTGGAATATGGCAAGA8070.1543782592560001No Hit
GTATCAACGCAGAGTACTTTTTTTT8060.15418696029781423No Hit
CTTTAGGACGTGAAATATGGCGAGG6220.11898795199161345No Hit
CTGTAGGACCTGGAATATGGCGAGA5420.10368403533674356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA359.618012E-519.0775531
TCCAACG555.989896E-717.2453318
TAGGACC2250.013.5363034
GTTCTAA500.001451943213.3542851
GTCTAGA500.001451943213.3542851
GGACCAT500.001479552113.3209616
GTATTAG751.4136387E-512.7183681
CCTACAC751.42769495E-512.7061423
TTAGGAC1900.012.5389573
TGTAGGA5750.012.4299212
TGAGTTA1700.012.27461712
GAATAAG701.04760125E-412.2641411
TAGGGTG701.0723368E-412.2335375
AGGACGT3350.012.2132495
AGTAGGG550.002993150612.1285912
ACACAAT1103.709465E-812.1099666
GTAGGAC5650.011.9752583
TGGCGAG4150.011.88473218
GGACGTG3300.011.8216326
CTGTAGG5750.011.7783151
Produced by FastQC (version 0.11.5)