##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926858_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 522742 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.001270225082354 31.0 28.0 31.0 16.0 34.0 2 28.143487609566478 31.0 28.0 31.0 16.0 34.0 3 27.61024558960252 31.0 27.0 31.0 15.0 34.0 4 31.21573548710454 35.0 32.0 37.0 17.0 37.0 5 31.5884508992964 35.0 32.0 37.0 17.0 37.0 6 30.84585895145215 35.0 31.0 37.0 11.0 37.0 7 30.888246974606975 35.0 32.0 37.0 11.0 37.0 8 30.786037854237847 35.0 32.0 37.0 10.0 37.0 9 32.788031189382146 37.0 32.0 39.0 17.0 39.0 10 32.965415061349574 37.0 33.0 39.0 17.0 39.0 11 33.1377696837063 37.0 33.0 39.0 17.0 39.0 12 32.99636149381531 37.0 33.0 39.0 16.0 39.0 13 33.109518653561416 38.0 33.0 39.0 17.0 39.0 14 34.09427021360442 39.0 34.0 41.0 17.0 41.0 15 33.674116485761616 39.0 33.0 41.0 10.0 41.0 16 33.75715553753094 39.0 33.0 40.0 11.0 41.0 17 33.970075103970984 39.0 33.0 41.0 11.0 41.0 18 34.00251940727931 39.0 34.0 41.0 11.0 41.0 19 33.38849375026304 39.0 33.0 40.0 10.0 41.0 20 33.678342279747945 39.0 33.0 41.0 10.0 41.0 21 33.66061843127202 39.0 33.0 41.0 2.0 41.0 22 33.67914382238274 39.0 34.0 41.0 2.0 41.0 23 33.66493604875828 39.0 34.0 41.0 2.0 41.0 24 32.35884432473381 38.0 32.0 40.0 2.0 41.0 25 32.922261842362005 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.8098576748034105 1101 2 -0.9960308468439081 1101 3 -1.9752655340363106 1101 4 -1.6113816853368483 1101 5 -1.006074342910665 1101 6 -3.142043210958281 1101 7 -1.770045487767085 1101 8 -1.1428979308185347 1101 9 -1.2405865434100285 1101 10 -9.012758904612639 1101 11 -4.385710966896259 1101 12 -2.218552712781424 1101 13 -1.655965239426461 1101 14 -1.8507000711249333 1101 15 -3.014681498581833 1101 16 -1.4424807341045778 1101 17 -1.4432074844175133 1101 18 -1.3617398740698974 1101 19 -2.162107473422342 1101 20 -1.416594213823224 1101 21 -1.1491806799195814 1101 22 -1.1244313326672852 1101 23 -1.109184959783974 1101 24 -1.6353836949055989 1101 25 -1.290265301856472 1102 1 -0.47888243651260254 1102 2 -0.5263489672128543 1102 3 -0.8662558312794957 1102 4 -0.9065611093319212 1102 5 -0.3766443584829915 1102 6 -1.4071423775821685 1102 7 -0.4148304698845777 1102 8 0.07708427223774805 1102 9 0.06054801175194058 1102 10 -0.0700855983169717 1102 11 -0.35475797478509463 1102 12 -0.47400730490627296 1102 13 -0.5605890223037449 1102 14 -0.5786686526742884 1102 15 0.07759736583625454 1102 16 -0.05502415693599971 1102 17 -0.35294275487972016 1102 18 -0.6817032534357139 1102 19 -0.36439882992592487 1102 20 -0.3249382424352376 1102 21 -0.3957111315170252 1102 22 -0.4253228910977285 1102 23 -0.4969649621797174 1102 24 -4.084066892194986 1102 25 -2.328663919170843 1103 1 -0.47898769716854517 1103 2 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-1.929215848296728 2216 15 -3.050423609995935 2216 16 -1.8459702909053881 2216 17 -2.2135949465852605 2216 18 -2.0202337759607616 2216 19 -1.3818611517658148 2216 20 -2.180556935947223 2216 21 -4.930442853663251 2216 22 -3.5182643014126 2216 23 -3.224407758021968 2216 24 -3.7523295460937547 2216 25 -3.333010499782816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33326.0 3 2.0 4 235.0 5 1351.0 6 1886.0 7 1497.0 8 1151.0 9 1112.0 10 1049.0 11 974.0 12 993.0 13 997.0 14 1001.0 15 1106.0 16 1112.0 17 1243.0 18 1357.0 19 1706.0 20 2294.0 21 2967.0 22 3628.0 23 4207.0 24 4985.0 25 5639.0 26 6605.0 27 7010.0 28 8188.0 29 9342.0 30 11272.0 31 13530.0 32 17726.0 33 22522.0 34 30173.0 35 41743.0 36 59189.0 37 93128.0 38 126496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.167879881215526 18.790606901232128 12.381306590851143 25.6602066267012 2 13.822173864737817 20.01578429156498 42.882702025523045 23.279339818174154 3 19.028581280410712 25.190605723918623 29.14869162101069 26.632121374659974 4 11.776203320653869 16.865345628648058 39.23295307705119 32.12549797364688 5 12.136444349197117 38.39032865430833 37.00690373046367 12.46632326603089 6 30.61012058520755 38.10316521818996 19.26225273526744 12.024461461335049 7 27.902046517160468 31.29461827572248 22.756687585255563 18.046647621861492 8 23.96719424460432 39.08239808153478 20.8715587529976 16.078848920863308 9 26.05122572843399 15.76532447553518 20.4298277168464 37.75362207918443 10 13.94274931871766 29.984652010165654 35.574420527266604 20.498178143850087 11 34.106828866026724 22.97354438638551 24.674686070383757 18.244940677204013 12 22.529743340514848 25.494701877781562 32.572449764464125 19.403105017239465 13 28.637328996460347 22.60690710800727 25.782454797665743 22.97330909786664 14 19.88917823132896 23.06114522205491 28.08773134905222 28.96194519756391 15 22.639025743559326 32.035664061378924 23.270991380547397 22.054318814514353 16 21.083517816585697 29.018944532566955 28.866255541672803 21.031282109174544 17 20.939701588492138 29.41274390873919 28.417592426057244 21.229962076711427 18 20.705852190433916 28.647167656158224 30.30981959556805 20.337160557839816 19 23.227085382328067 27.538680555821315 27.540785359749258 21.69344870210136 20 22.575769171896525 28.368092759834685 28.803000153069036 20.253137915199755 21 22.269906018490175 27.50202810261434 27.522500459193044 22.70556541970244 22 21.92852045101877 29.046818167033077 28.049188828235167 20.975472553712983 23 21.496906080307976 28.68486409331124 28.281334307876584 21.536895518504203 24 22.734291142655426 27.685587514421666 28.792253339226363 20.787868003696538 25 21.844470720591445 28.141179441848717 28.652033658978983 21.36231617858086 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 199.0 1 199.0 2 261.5 3 324.0 4 324.0 5 324.0 6 454.5 7 585.0 8 585.0 9 585.0 10 962.5 11 1340.0 12 1340.0 13 1340.0 14 2109.5 15 2879.0 16 2879.0 17 2879.0 18 4994.5 19 7110.0 20 7110.0 21 7110.0 22 10973.5 23 14837.0 24 14837.0 25 14837.0 26 20789.5 27 26742.0 28 26742.0 29 26742.0 30 34808.5 31 42875.0 32 42875.0 33 42875.0 34 48687.0 35 54499.0 36 54499.0 37 54499.0 38 58564.0 39 62629.0 40 62629.0 41 62629.0 42 65089.0 43 67549.0 44 67549.0 45 67549.0 46 68378.0 47 69207.0 48 69207.0 49 69207.0 50 65224.5 51 61242.0 52 61242.0 53 61242.0 54 53109.5 55 44977.0 56 44977.0 57 44977.0 58 37859.5 59 30742.0 60 30742.0 61 30742.0 62 24650.5 63 18559.0 64 18559.0 65 18559.0 66 14175.5 67 9792.0 68 9792.0 69 9792.0 70 6971.5 71 4151.0 72 4151.0 73 4151.0 74 2907.0 75 1663.0 76 1663.0 77 1663.0 78 1107.0 79 551.0 80 551.0 81 551.0 82 381.0 83 211.0 84 211.0 85 211.0 86 134.0 87 57.0 88 57.0 89 57.0 90 38.0 91 19.0 92 19.0 93 19.0 94 10.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2152113279591079 2 0.1346744665628551 3 0.13811784781020084 4 0.07173710931970265 5 0.02410366873142009 6 0.02372107081504834 7 0.2898179216515987 8 0.28541804561332357 9 0.021808081233189603 10 0.037877193720803 11 0.017790803111286255 12 0.019895091651330868 13 0.01759950415310038 14 0.019321194776773246 15 0.016643009362171016 16 0.021234184358631985 17 0.02066028748407436 18 0.015877813529427517 19 0.024486266647791837 20 0.01951249373495912 21 0.01645171040398514 22 0.01970379269314499 23 0.019895091651330868 24 0.017408205194914508 25 0.016834308320356886 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 522742.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.54365511976309 #Duplication Level Percentage of deduplicated Percentage of total 1 94.46015594861113 82.6938731492425 2 4.261290564576723 7.460979031008104 3 0.6120541277132914 1.6074436641347944 4 0.22305787131288038 0.7810920543185319 5 0.11465209683818062 0.5018531812179683 6 0.06825995403042341 0.3585435524478161 7 0.04722065304282961 0.28937079951580263 8 0.030384869805696407 0.2128002050503031 9 0.028257357183640877 0.22263770986925552 >10 0.12884192609092301 2.126172928120008 >50 0.012912323980239514 0.7780543677434435 >100 0.01138033820902525 1.90231315548349 >500 0.0013131159471887895 0.8652277379535188 >1k 2.1885265786479827E-4 0.19963846389450102 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1042 0.19933351442968042 No Hit GTCCTACAGTGTGCATTTCTCATTT 916 0.17522984569826033 No Hit GTCCTAAAGTGTGTATTTCTCATTT 823 0.15743904258697405 No Hit CTGTAGGACGTGGAATATGGCAAGA 807 0.1543782592560001 No Hit GTATCAACGCAGAGTACTTTTTTTT 806 0.15418696029781423 No Hit CTTTAGGACGTGAAATATGGCGAGG 622 0.11898795199161345 No Hit CTGTAGGACCTGGAATATGGCGAGA 542 0.10368403533674356 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.9129895818587373E-4 0.0 5 0.0 0.0 0.0 1.9129895818587373E-4 0.0 6 0.0 0.0 0.0 1.9129895818587373E-4 0.0 7 0.0 0.0 0.0 1.9129895818587373E-4 0.0 8 0.0 0.0 0.0 1.9129895818587373E-4 0.0 9 0.0 0.0 0.0 1.9129895818587373E-4 0.0 10 0.0 0.0 0.0 1.9129895818587373E-4 0.0 11 0.0 0.0 0.0 1.9129895818587373E-4 0.0 12 0.0 0.0 0.0 1.9129895818587373E-4 0.0 13 0.0 0.0 0.0 1.9129895818587373E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCCCTAA 35 9.618012E-5 19.077553 1 TCCAACG 55 5.989896E-7 17.24533 18 TAGGACC 225 0.0 13.536303 4 GTTCTAA 50 0.0014519432 13.354285 1 GTCTAGA 50 0.0014519432 13.354285 1 GGACCAT 50 0.0014795521 13.320961 6 GTATTAG 75 1.4136387E-5 12.718368 1 CCTACAC 75 1.42769495E-5 12.706142 3 TTAGGAC 190 0.0 12.538957 3 TGTAGGA 575 0.0 12.429921 2 TGAGTTA 170 0.0 12.274617 12 GAATAAG 70 1.04760125E-4 12.264141 1 TAGGGTG 70 1.0723368E-4 12.233537 5 AGGACGT 335 0.0 12.213249 5 AGTAGGG 55 0.0029931506 12.128591 2 ACACAAT 110 3.709465E-8 12.109966 6 GTAGGAC 565 0.0 11.975258 3 TGGCGAG 415 0.0 11.884732 18 GGACGTG 330 0.0 11.821632 6 CTGTAGG 575 0.0 11.778315 1 >>END_MODULE