FastQCFastQC Report
Thu 26 May 2016
SRR1926858_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926858_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522742
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12560.24027149148145738No Hit
GTCCTACAGTGGACATTTCTAAATT10580.20239429776065437No Hit
CTGTAGGACGTGGAATATGGCAAGA10230.1956988342241488No Hit
GTCCTACAGTGTGCATTTCTCATTT9170.1754211446564462No Hit
GGTATCAACGCAGAGTACTTTTTTT8600.1645171040398514No Hit
CTTTAGGACGTGAAATATGGCGAGG8200.15686514571241647No Hit
GTCCTAAAGTGTGTATTTCTCATTT7790.14902188842679565No Hit
CTGTAGGACCTGGAATATGGCGAGA7540.14423941447214877No Hit
TATCAACGCAGAGTACTTTTTTTTT6790.12989199260820825No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGAGT508.559271E-515.2283194
GTGTTAA456.6135806E-414.8181041
TCTAGAC456.647371E-414.8081523
TAGGACC2450.014.7621464
CCGGTTT400.005308437614.2355812
GGCGAGG2300.014.02926719
CCAACGT551.9731096E-413.80419919
GTAGGAC6350.013.4922423
TTACACC500.001476362213.32477954
AATCCCG1001.0157237E-813.2865419
TCCAACG655.490906E-513.14053518
TGTAGGA6850.013.072072
TGTCCAA604.118985E-412.65384910
AGGACGT5800.012.1432715
TTGGACT550.003021327812.1134364
AGGACTC550.003021327812.1134365
TAAGCTT550.003021327812.1134364
GGACGTG5600.012.0659276
GTGTAGA951.0018339E-612.0327461
CTAGGAC951.010003E-612.0246653
Produced by FastQC (version 0.11.5)