FastQCFastQC Report
Thu 26 May 2016
SRR1926857_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926857_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632582
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11090.17531324002263737No Hit
GTCCTAAAGTGTGTATTTCTCATTT9890.15634336734209953No Hit
GTCCTACAGTGTGCATTTCTCATTT8810.13927048192961544No Hit
GTATCAACGCAGAGTACTTTTTTTT8230.13010171013402216No Hit
CTGTAGGACGTGGAATATGGCAAGA7850.12409458378518518No Hit
CTTTAGGACGTGAAATATGGCGAGG7050.11144800199815992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGCTG250.0059638619.0353225
AGATAGC400.005209896214.2764926
CCAACGA400.00534023214.22342819
TAGGACC2300.013.6579444
GGCGAGG1750.013.00427719
TAGCACA905.2577525E-712.6922314
TCCAACG550.003108125612.06836318
CTTATCC657.7834504E-411.7326723
TTAGGCT657.87743E-411.7159054
TGGCGAG4250.011.60185518
CCCGTTT752.1078499E-411.37784312
CTGGACA1104.822323E-711.2499324
GTAGGAC5650.011.1356543
GTGTTAG951.2885119E-511.0511521
GTAGCAC700.00144705510.8946243
TAAGATC700.001464429710.8790554
TTAGCAC803.645966E-410.7243953
TCTAAAT4000.010.66757118
ATTTCTA4000.010.66757115
GGCGAGA2850.010.64677719
Produced by FastQC (version 0.11.5)