##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926857_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 632582 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.692158486962956 31.0 28.0 31.0 16.0 34.0 2 27.83082983708041 31.0 28.0 31.0 16.0 34.0 3 27.315287188064158 31.0 27.0 31.0 10.0 34.0 4 30.863231960441492 35.0 32.0 37.0 16.0 37.0 5 31.2618300868504 35.0 32.0 37.0 16.0 37.0 6 30.44576987647451 35.0 30.0 37.0 10.0 37.0 7 30.503604275809302 35.0 31.0 37.0 10.0 37.0 8 30.407814322886203 35.0 30.0 37.0 10.0 37.0 9 32.40666980723448 37.0 32.0 39.0 11.0 39.0 10 32.58122267152717 37.0 32.0 39.0 11.0 39.0 11 32.760078535272896 37.0 33.0 39.0 11.0 39.0 12 32.6270570455688 37.0 33.0 39.0 10.0 39.0 13 32.7173362504782 37.0 33.0 39.0 10.0 39.0 14 33.696127932821355 39.0 33.0 40.0 10.0 41.0 15 33.261076034411346 38.0 32.0 40.0 10.0 41.0 16 33.35023759765532 38.0 33.0 40.0 10.0 41.0 17 33.56777145097394 39.0 33.0 40.0 8.0 41.0 18 33.60297637302357 39.0 33.0 40.0 2.0 41.0 19 32.96852423875482 39.0 32.0 40.0 2.0 41.0 20 33.25208431476077 39.0 33.0 40.0 2.0 41.0 21 33.25168594743448 39.0 33.0 40.0 2.0 41.0 22 33.25399711025606 39.0 33.0 40.0 2.0 41.0 23 33.230292673519 39.0 33.0 40.0 2.0 41.0 24 31.913361429822537 38.0 31.0 40.0 2.0 41.0 25 32.474001789491325 38.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.41261652353929534 1101 2 -0.5255022906208282 1101 3 -1.5199766350943378 1101 4 -0.9133460787241745 1101 5 -0.24532487179412854 1101 6 -2.0400923704179625 1101 7 -0.5631922581977022 1101 8 0.10755981816191351 1101 9 -0.02243129591919768 1101 10 -9.165751255315694 1101 11 -4.018235958622306 1101 12 -1.6672821375836406 1101 13 -0.9426043360389436 1101 14 -0.8208341506421419 1101 15 -2.0621599259300787 1101 16 -0.33815881672203574 1101 17 -0.3123794526781296 1101 18 -0.1125757751162837 1101 19 -0.7540080232093231 1101 20 -0.006790122147435795 1101 21 -0.24240105711614746 1101 22 0.1423249717013917 1101 23 -0.03501957138205114 1101 24 -0.2732431890803291 1101 25 -0.035442183445624664 1102 1 -0.4263731372959114 1102 2 -0.3743877099980537 1102 3 -0.6193954918902449 1102 4 -0.7617631140264542 1102 5 -0.08605693875570353 1102 6 -1.3641447600441232 1102 7 -0.38253478573695077 1102 8 0.24004312113702042 1102 9 -0.21073904160399337 1102 10 0.12938275457241843 1102 11 -0.32292847315088125 1102 12 -0.03890153215385794 1102 13 -0.3287956796073317 1102 14 -0.6211811018087658 1102 15 0.08609230756805175 1102 16 -0.227984473760813 1102 17 -0.42470489287241975 1102 18 -0.7364460153799683 1102 19 -0.11651994965567525 1102 20 -0.23064301485278804 1102 21 -0.15951442340984556 1102 22 -0.5911991847735862 1102 23 -1.0235354877668215 1102 24 -4.546935616871117 1102 25 -2.5433439962654703 1103 1 0.37369862445507707 1103 2 0.004039060664332794 1103 3 0.29360456104875254 1103 4 0.15695082128209137 1103 5 -0.039308275465369746 1103 6 0.5930570022362538 1103 7 0.3169234718642038 1103 8 0.691684524138072 1103 9 0.4218878502298722 1103 10 0.44346158662050783 1103 11 0.2803396275743779 1103 12 -0.04404955547054357 1103 13 0.241613774121042 1103 14 0.27794305190255386 1103 15 0.7366349322835077 1103 16 0.7522234000326193 1103 17 0.41139362071819363 1103 18 0.09368686150157401 1103 19 -1.5851230071768327 1103 20 0.01677620906152555 1103 21 0.09880670454371199 1103 22 0.2853176645326201 1103 23 0.32178441082559317 1103 24 1.8310676418182261 1103 25 1.0120037247107234 1104 1 -0.5577332500513705 1104 2 -0.40426444147600193 1104 3 -1.7068699312899582 1104 4 -1.0486067697988197 1104 5 -0.6428136018409987 1104 6 -1.9200603782929484 1104 7 -0.5885376277291208 1104 8 -0.2291640092595877 1104 9 -0.17192736401991482 1104 10 -0.17783890926381218 1104 11 -0.18395852201928875 1104 12 -0.26347552214912184 1104 13 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22 0.9557024324472678 1108 23 1.013231397921551 1108 24 0.005482471224276253 1108 25 0.6497601385988006 1109 1 0.7783303391867058 1109 2 0.7401451271738289 1109 3 -0.12676080752268248 1109 4 0.8192017188542984 1109 5 1.0217475468120334 1109 6 1.2913582739161171 1109 7 1.3997609667187128 1109 8 1.6844059894866135 1109 9 1.2700347020682727 1109 10 1.3594508091501751 1109 11 1.0558673295914147 1109 12 0.6395020348447034 1109 13 0.7740639536354834 1109 14 0.9026312955226885 1109 15 -0.644384744964718 1109 16 0.699683232162954 1109 17 0.628270399628164 1109 18 0.8150575549710339 1109 19 -0.9922817921579323 1109 20 0.3642813875743798 1109 21 0.5868163285429517 1109 22 0.8651184926544175 1109 23 0.8993784550405195 1109 24 -0.44443804739175263 1109 25 0.23388621594412484 1110 1 0.38441799146939815 1110 2 0.6143609944261712 1110 3 0.966949275068913 1110 4 0.8877362917722991 1110 5 0.8306344184650634 1110 6 0.7296359335705205 1110 7 1.0996115017262156 1110 8 1.3215360585532494 1110 9 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0.10979269397777713 2215 3 0.7086773272643612 2215 4 1.1114700625265073 2215 5 0.7359075345079127 2215 6 1.5974516598837276 2215 7 0.9624094278496642 2215 8 -0.4991047851600712 2215 9 0.15503108488579898 2215 10 -0.09277959682871284 2215 11 -0.2699610621579893 2215 12 -0.4294551342970294 2215 13 0.14059561679889754 2215 14 -0.06484824033868364 2215 15 -0.09620216554335315 2215 16 0.19576699129847697 2215 17 -0.13334303464449704 2215 18 -0.874969844474144 2215 19 0.1957347956142783 2215 20 -3.5493790301959507 2215 21 -2.1580014167276502 2215 22 -1.0409914127292055 2215 23 -0.912117919232486 2215 24 -0.9350020430397414 2215 25 -0.5613356853836962 2216 1 -1.066398152930681 2216 2 -1.5962230784147877 2216 3 -0.5639991888729874 2216 4 -0.8784026120733905 2216 5 -0.9300762541064884 2216 6 -2.359433823905757 2216 7 -9.826966243922907 2216 8 -10.937871354098526 2216 9 -6.376293332708066 2216 10 -4.082848250461467 2216 11 -3.146377555056585 2216 12 -2.6071244993772247 2216 13 -2.5489465936007107 2216 14 -2.7492139912171076 2216 15 -3.7149349738270807 2216 16 -2.88721924139222 2216 17 -2.891331575532689 2216 18 -2.6708999261233615 2216 19 -2.1174693134998783 2216 20 -2.8341862129581585 2216 21 -5.2484296265593535 2216 22 -3.996257674198329 2216 23 -3.794164671990565 2216 24 -4.0095515961204455 2216 25 -3.626925948100123 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44845.0 3 5.0 4 275.0 5 1764.0 6 2365.0 7 1876.0 8 1637.0 9 1459.0 10 1380.0 11 1304.0 12 1291.0 13 1253.0 14 1267.0 15 1338.0 16 1420.0 17 1555.0 18 1791.0 19 2193.0 20 2812.0 21 3640.0 22 4509.0 23 5403.0 24 6447.0 25 7127.0 26 8065.0 27 8855.0 28 9941.0 29 11755.0 30 14033.0 31 16715.0 32 21436.0 33 27807.0 34 37122.0 35 51209.0 36 72559.0 37 111917.0 38 142212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48604830250386 19.25017350492627 12.722268525414115 25.541509667155754 2 13.737420422996685 20.48029529359997 42.52775500254836 23.25452928085498 3 18.43542346643198 25.730417467337386 29.785108949256205 26.04905011697443 4 11.933217642210703 17.314911341262974 39.498815932470976 31.253055084055347 5 12.135931540899 38.16433599605335 37.35698157600137 12.342750887046277 6 29.910474287051503 38.20274996679548 19.8223083782912 12.064467367861818 7 27.315118708994106 31.479843095471395 23.379821215090487 17.825216980444015 8 23.74029087346069 38.553972358618154 21.407880822106765 16.297855945814394 9 25.816764564047023 15.739368976939435 20.925742926486052 37.51812353252749 10 13.964013827697777 29.827596567745278 35.75497268934805 20.453416915208887 11 33.41839356324892 23.097330721446717 25.260200508147918 18.224075207156442 12 22.3796799898811 25.28506830255502 32.928788262079436 19.40646344548444 13 28.36894345910975 22.828372579834245 25.99597152876373 22.806712432292276 14 19.99326464110498 22.873025836267537 28.36677033216703 28.766939190460455 15 22.438735177865613 31.886798418972333 23.66798418972332 22.006482213438737 16 21.175693046685883 28.72351022434585 29.0068478530692 21.09394887589907 17 20.87712973854969 29.139155567361087 28.58086998317712 21.402844710912102 18 20.742865183550276 28.609621863819118 30.275716722767065 20.37179622986354 19 23.215753987830947 27.355381832441527 27.68807002896791 21.74079415075962 20 22.602504731352465 28.38273914081485 28.594918756709664 20.419837371123016 21 22.381855839618016 27.589527107147937 27.49845847365176 22.530158579582285 22 22.067889278717917 28.991052554188258 28.019308395167524 20.9217497719263 23 21.644786875603785 28.419484845945508 28.20477144406621 21.7309568343845 24 22.834877223937657 27.73232272926341 28.55256896123752 20.88023108556142 25 22.141043438115627 27.977852474204962 28.571383398209605 21.309720689469806 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 235.0 3 285.0 4 285.0 5 285.0 6 446.5 7 608.0 8 608.0 9 608.0 10 970.5 11 1333.0 12 1333.0 13 1333.0 14 2357.5 15 3382.0 16 3382.0 17 3382.0 18 5978.0 19 8574.0 20 8574.0 21 8574.0 22 13323.5 23 18073.0 24 18073.0 25 18073.0 26 25994.5 27 33916.0 28 33916.0 29 33916.0 30 44200.5 31 54485.0 32 54485.0 33 54485.0 34 61186.5 35 67888.0 36 67888.0 37 67888.0 38 72707.5 39 77527.0 40 77527.0 41 77527.0 42 79490.0 43 81453.0 44 81453.0 45 81453.0 46 81198.0 47 80943.0 48 80943.0 49 80943.0 50 76546.5 51 72150.0 52 72150.0 53 72150.0 54 63127.0 55 54104.0 56 54104.0 57 54104.0 58 45462.0 59 36820.0 60 36820.0 61 36820.0 62 29284.0 63 21748.0 64 21748.0 65 21748.0 66 16525.5 67 11303.0 68 11303.0 69 11303.0 70 8133.5 71 4964.0 72 4964.0 73 4964.0 74 3423.5 75 1883.0 76 1883.0 77 1883.0 78 1260.0 79 637.0 80 637.0 81 637.0 82 431.5 83 226.0 84 226.0 85 226.0 86 148.0 87 70.0 88 70.0 89 70.0 90 44.5 91 19.0 92 19.0 93 19.0 94 12.0 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.23332943397061565 2 0.12709814695960364 3 0.12962746331700872 4 0.069398117556301 5 0.023080011761321063 6 0.02181535358261854 7 0.29719467199509314 8 0.2941911088206746 9 0.018495625863524413 10 0.03604275809302193 11 0.014069322238065578 12 0.01612439177845718 13 0.013278910876376502 14 0.015650144961443735 15 0.01296274633170087 16 0.019127954952875673 17 0.017389049957159702 18 0.013436993148714316 19 0.02497699902937485 20 0.015650144961443735 21 0.014543569055079026 22 0.01754713222949752 23 0.01754713222949752 24 0.014069322238065578 25 0.015175898144430287 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 632582.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.27022526144921 #Duplication Level Percentage of deduplicated Percentage of total 1 93.91874795363371 81.96310290186888 2 4.69266214677073 8.19059365249115 3 0.7036718309611351 1.84228797594344 4 0.23215133795335569 0.810395982317447 5 0.11933037612013306 0.5206994402268736 6 0.07449596804462194 0.39007679473943274 7 0.046112108177793036 0.2816949847569419 8 0.031169040881263398 0.2176103375112941 9 0.02612205531639599 0.20517098865785233 >10 0.13270745521047703 2.1913808086157855 >50 0.011777327327502711 0.708237990161965 >100 0.009784091451940832 1.7518358228144386 >500 0.0010871212721906677 0.7515548032170795 >1k 1.8118687869844464E-4 0.1753575166774124 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1109 0.17531324002263737 No Hit GTCCTAAAGTGTGTATTTCTCATTT 989 0.15634336734209953 No Hit GTCCTACAGTGTGCATTTCTCATTT 881 0.13927048192961544 No Hit GTATCAACGCAGAGTACTTTTTTTT 823 0.13010171013402216 No Hit CTGTAGGACGTGGAATATGGCAAGA 785 0.12409458378518518 No Hit CTTTAGGACGTGAAATATGGCGAGG 705 0.11144800199815992 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.580822723378155E-4 0.0 10 0.0 0.0 0.0 1.580822723378155E-4 0.0 11 0.0 0.0 0.0 1.580822723378155E-4 0.0 12 0.0 0.0 0.0 1.580822723378155E-4 0.0 13 0.0 0.0 0.0 1.580822723378155E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGCTG 25 0.00596386 19.035322 5 AGATAGC 40 0.0052098962 14.276492 6 CCAACGA 40 0.005340232 14.223428 19 TAGGACC 230 0.0 13.657944 4 GGCGAGG 175 0.0 13.004277 19 TAGCACA 90 5.2577525E-7 12.692231 4 TCCAACG 55 0.0031081256 12.068363 18 CTTATCC 65 7.7834504E-4 11.732672 3 TTAGGCT 65 7.87743E-4 11.715905 4 TGGCGAG 425 0.0 11.601855 18 CCCGTTT 75 2.1078499E-4 11.377843 12 CTGGACA 110 4.822323E-7 11.249932 4 GTAGGAC 565 0.0 11.135654 3 GTGTTAG 95 1.2885119E-5 11.051152 1 GTAGCAC 70 0.001447055 10.894624 3 TAAGATC 70 0.0014644297 10.879055 4 TTAGCAC 80 3.645966E-4 10.724395 3 TCTAAAT 400 0.0 10.667571 18 ATTTCTA 400 0.0 10.667571 15 GGCGAGA 285 0.0 10.646777 19 >>END_MODULE