FastQCFastQC Report
Thu 26 May 2016
SRR1926857_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926857_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632582
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13410.2119883272050106No Hit
GTCCTACAGTGGACATTTCTAAATT10850.1715192654865298No Hit
CTGTAGGACGTGGAATATGGCAAGA10090.15950501278885582No Hit
GTCCTAAAGTGTGTATTTCTCATTT9400.14859733599754657No Hit
CTTTAGGACGTGAAATATGGCGAGG9000.14227404510403394No Hit
GTCCTACAGTGTGCATTTCTCATTT8760.13848007056792638No Hit
GGTATCAACGCAGAGTACTTTTTTT8630.13642500102753477No Hit
CTGTAGGACCTGGAATATGGCGAGA7570.11966828015972633No Hit
TATCAACGCAGAGTACTTTTTTTTT7540.11919403334271289No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATC400.00530975314.2357077
TAGGACC3000.013.9602044
TAATAGT852.6280395E-713.4376314
GGTATCA2750.013.1672111
GTAGGAC6850.012.9226863
GGACCTG4050.012.6910966
CAAGACA604.0201578E-412.6910954
TGTAGGA7350.012.5755592
AGGACCT4400.012.5468795
GGCGAGG2950.012.22598119
CTGTAGG7200.012.175821
GTCATAC550.002996014212.1276941
GTGTTAG657.810192E-411.72788051
GTCCTAG907.19333E-611.6464371
TGAGTTA2400.011.464931512
TGGCGAG6500.011.38946518
CTAAAGT2950.011.2929244
GGACTGT855.20778E-511.1980266
CTACTAC600.00573785811.1170531
CTTAGAC600.00578552411.1047093
Produced by FastQC (version 0.11.5)