##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926857_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 632582 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.916776006905035 31.0 30.0 33.0 26.0 34.0 2 30.230782728563252 31.0 30.0 34.0 26.0 34.0 3 30.284682460139557 31.0 30.0 34.0 26.0 34.0 4 33.83937418390027 37.0 35.0 37.0 29.0 37.0 5 33.81501844820118 37.0 35.0 37.0 30.0 37.0 6 33.73738898672425 37.0 35.0 37.0 29.0 37.0 7 33.72613827140197 37.0 35.0 37.0 29.0 37.0 8 33.75008773566115 37.0 35.0 37.0 29.0 37.0 9 35.30985548118663 39.0 35.0 39.0 30.0 39.0 10 35.19278449276141 39.0 35.0 39.0 29.0 39.0 11 35.22148749094979 39.0 35.0 39.0 29.0 39.0 12 35.12027057361733 39.0 35.0 39.0 29.0 39.0 13 35.14261708363501 39.0 35.0 39.0 29.0 39.0 14 36.24626372549329 40.0 36.0 41.0 29.0 41.0 15 36.22064175079278 40.0 36.0 41.0 29.0 41.0 16 36.197179179932405 40.0 36.0 41.0 29.0 41.0 17 36.17551400450851 40.0 36.0 41.0 29.0 41.0 18 36.13988226032356 40.0 36.0 41.0 29.0 41.0 19 36.21386318295494 40.0 37.0 41.0 29.0 41.0 20 36.17961465865295 40.0 37.0 41.0 29.0 41.0 21 36.13085576257308 40.0 37.0 41.0 28.0 41.0 22 36.03751292322576 40.0 36.0 41.0 28.0 41.0 23 35.97530122576994 40.0 36.0 41.0 28.0 41.0 24 35.89789940276518 40.0 36.0 41.0 27.0 41.0 25 35.782801281098735 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12739412495325197 1101 2 0.1506674008013107 1101 3 0.4428232669480323 1101 4 0.39345622154012716 1101 5 0.2946615985354697 1101 6 0.31762464700573645 1101 7 0.17475221642398253 1101 8 0.2858873489064848 1101 9 0.47145432022416855 1101 10 0.42514264724518114 1101 11 0.4856465604956668 1101 12 0.4359938428747654 1101 13 0.4452758334364191 1101 14 0.5255807039447831 1101 15 0.4150614772070824 1101 16 0.5491122630464886 1101 17 0.35620283380763595 1101 18 0.22038994382756272 1101 19 0.36830023204220197 1101 20 0.22857554206266428 1101 21 0.502753462615388 1101 22 0.43113427113977565 1101 23 0.34987631774369987 1101 24 0.30226760687353504 1101 25 0.3745956755910598 1102 1 -0.6902656894278714 1102 2 -0.7412399632371987 1102 3 -0.5692506336504586 1102 4 -0.49418364314891505 1102 5 -0.774850067697507 1102 6 -0.7417907402785033 1102 7 -0.7156806051563791 1102 8 -0.635354736270024 1102 9 -0.6682976096916988 1102 10 -0.803480824001241 1102 11 -0.7944644638776523 1102 12 -0.5669031993665428 1102 13 -0.5017273758618757 1102 14 -0.7273120014069434 1102 15 -0.4680853698741885 1102 16 -0.6285787769396336 1102 17 -0.7092841988925116 1102 18 -0.5810499035139287 1102 19 -0.4864313144926271 1102 20 -0.5816074746777318 1102 21 -0.7326529039385221 1102 22 -0.6435643150342187 1102 23 -0.764652262358652 1102 24 -0.7920337202146825 1102 25 -0.5529609208179949 1103 1 -0.3796220100499639 1103 2 -0.4162333458626222 1103 3 -0.0639137945791255 1103 4 -0.30496672855128537 1103 5 -0.11135385871787662 1103 6 -0.08452251014680456 1103 7 0.026279431783272855 1103 8 -0.1596449125568853 1103 9 -0.09165900069324096 1103 10 0.15233864225603355 1103 11 0.18005540883346782 1103 12 0.02026321142830767 1103 13 0.17874813397007472 1103 14 0.2393823111801865 1103 15 0.04171128555764625 1103 16 0.19283681183024015 1103 17 0.1334999217613415 1103 18 0.12160704590580451 1103 19 0.23169205689796257 1103 20 0.27302909911040985 1103 21 0.16043738057917523 1103 22 0.11503856602362106 1103 23 -0.039815049773288536 1103 24 -0.21537040635113414 1103 25 -0.1169518153105713 1104 1 -0.3007891474160651 1104 2 -0.5273419283708094 1104 3 -0.2757444702243568 1104 4 -0.18927138676887267 1104 5 -0.18431644323327134 1104 6 -0.4245400788799216 1104 7 -0.5270890086730589 1104 8 -0.4076314492634765 1104 9 -0.6520173846428818 1104 10 -0.4106505001758691 1104 11 -0.6335464773020263 1104 12 -0.46105863092189736 1104 13 -0.31545327380431587 1104 14 -0.2966392810659002 1104 15 -0.40068625481971765 1104 16 -0.5363145409849679 1104 17 -0.6549698006208118 1104 18 -0.6056030984445542 1104 19 -0.4706328417745951 1104 20 -0.4621814184617392 1104 21 -0.5444841404361753 1104 22 -0.6878104361770241 1104 23 -0.5976644274714644 1104 24 -0.6240084426583294 1104 25 -0.47129177029638214 1105 1 -0.19285418777940322 1105 2 -0.3296653029134369 1105 3 -0.3201684154591007 1105 4 -0.26456478783787674 1105 5 -0.19017343034966672 1105 6 -0.40903693852850154 1105 7 -0.725293071930281 1105 8 -0.44051576163023753 1105 9 -0.43298725227585777 1105 10 -0.36356729741465443 1105 11 -0.47347241532477113 1105 12 -0.5148948030034433 1105 13 -0.41949093153911576 1105 14 -0.44901363390633975 1105 15 -0.5014997007319622 1105 16 -0.32831867221325695 1105 17 -0.2788713396799025 1105 18 -0.3809278368221598 1105 19 -0.6037237155558586 1105 20 -0.44254042926207404 1105 21 -0.7069835335516714 1105 22 -0.646926282703653 1105 23 -0.6144828520434444 1105 24 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0.20137341027797362 1107 11 0.0904541236788603 1107 12 0.1086175906254141 1107 13 0.007723124817715643 1107 14 0.2638505183567261 1107 15 -0.01630317243167667 1107 16 0.26530488786855244 1107 17 0.02851502997769728 1107 18 -0.17816510142799302 1107 19 0.016800218613255424 1107 20 0.08721169816075047 1107 21 -0.0794504962383229 1107 22 0.0018269362486904583 1107 23 0.03115367922410428 1107 24 -0.12746408275663157 1107 25 -0.17807534205457642 1108 1 0.7856540911079577 1108 2 0.6242873481054687 1108 3 0.7070961285893134 1108 4 0.7060132551497915 1108 5 0.6783976480609937 1108 6 0.818862785085976 1108 7 0.6155443677424515 1108 8 0.5723674380181549 1108 9 0.6935373054650498 1108 10 0.5725174946200298 1108 11 0.5741745042867663 1108 12 0.5338678012750364 1108 13 0.5890626509074437 1108 14 0.6255153696689177 1108 15 0.599285927221004 1108 16 0.5147078760105188 1108 17 0.6887125268436449 1108 18 0.5627533309277908 1108 19 0.7021873001397907 1108 20 0.5579159871925867 1108 21 0.8018109353570679 1108 22 0.6209796109851169 1108 23 0.7577078624173481 1108 24 0.7576753570181225 1108 25 0.6883512182939739 1109 1 0.6222556823914971 1109 2 0.30394425346466747 1109 3 0.4272326544249019 1109 4 0.40921238085456935 1109 5 0.6303093409316745 1109 6 0.714456535862162 1109 7 0.4926669760565332 1109 8 0.4942003946428457 1109 9 0.6355241744780713 1109 10 0.33639982905451404 1109 11 0.3098098999964236 1109 12 0.2548239788828113 1109 13 0.5102643100813324 1109 14 0.3578266220802675 1109 15 0.3925536056194545 1109 16 0.3590775306804659 1109 17 0.4126575356611113 1109 18 0.5139985392766491 1109 19 0.6067330557510999 1109 20 0.6109366937489682 1109 21 0.5439973357610981 1109 22 0.6057601678515638 1109 23 0.5540409556506702 1109 24 0.39993047570204254 1109 25 0.6415544836541613 1110 1 0.3718774506679594 1110 2 0.1868112194674758 1110 3 0.4503357588696595 1110 4 0.7160583242812564 1110 5 0.6402225659435103 1110 6 0.6101315957192384 1110 7 0.6510267602258324 1110 8 0.3322979246119928 1110 9 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2214 14 0.25499869336277214 2214 15 5.641227097257229E-4 2214 16 0.1391651730993928 2214 17 0.264985902590702 2214 18 0.06430528774290423 2214 19 0.10904097278294245 2214 20 0.2734432669303928 2214 21 0.2045524044143292 2214 22 0.11706019706570459 2214 23 0.154426582293965 2214 24 0.20988665449257837 2214 25 0.4317385327339167 2215 1 0.44763406246338633 2215 2 0.3388002212461103 2215 3 0.32398345618621605 2215 4 0.5983198197184905 2215 5 0.652186174444445 2215 6 0.48049033800513286 2215 7 0.24502384101315755 2215 8 0.35545154103335364 2215 9 0.25403674347511895 2215 10 0.31264319992679646 2215 11 0.1747152551353892 2215 12 0.28767767951681833 2215 13 0.5253453976285414 2215 14 0.283933565361707 2215 15 0.1476048442908393 2215 16 0.2850306725025007 2215 17 0.4990540813942559 2215 18 0.35279182441885837 2215 19 0.13686959726894798 2215 20 0.2506710159687202 2215 21 0.12795869172033747 2215 22 0.3198404066425695 2215 23 0.370017509472639 2215 24 0.352459940074219 2215 25 0.4241247908583361 2216 1 -0.06062318745430417 2216 2 -3.793843918131756E-4 2216 3 -1.6609052538536595 2216 4 -1.5704259667323015 2216 5 -0.4339505300766575 2216 6 -0.038498723751779096 2216 7 -0.20794728822674102 2216 8 -0.032426676724611525 2216 9 0.08936035276434495 2216 10 0.19540680043616732 2216 11 0.20089021476371016 2216 12 0.09358440046531769 2216 13 0.02161918295049503 2216 14 -0.21103543511038225 2216 15 -0.1528371102036985 2216 16 0.20815114647561472 2216 17 -0.08459151186475822 2216 18 0.057050753659098064 2216 19 -0.17326939977133549 2216 20 0.006074375659061104 2216 21 0.09553069441700757 2216 22 -0.17123427269218183 2216 23 -0.07713957512584813 2216 24 -0.1265450438903386 2216 25 -0.2392433309308757 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15733.0 3 26.0 4 262.0 5 1655.0 6 2244.0 7 1637.0 8 1270.0 9 1106.0 10 943.0 11 924.0 12 824.0 13 796.0 14 813.0 15 801.0 16 855.0 17 830.0 18 913.0 19 1159.0 20 1434.0 21 1881.0 22 2398.0 23 2872.0 24 3559.0 25 4212.0 26 4926.0 27 5530.0 28 6366.0 29 7692.0 30 9972.0 31 12814.0 32 16621.0 33 22132.0 34 30064.0 35 42088.0 36 62011.0 37 107068.0 38 256151.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.134811260437175 19.92447885344593 10.60698618083295 28.333723705283948 2 13.738080353256784 21.64425839024429 40.48857692277256 24.129084333726368 3 17.979942353634996 25.507172592837367 29.90227312613739 26.61061192739024 4 11.69634924800263 18.202383248337764 38.97059985899061 31.130667644668993 5 12.430696849404075 39.02318720980417 35.86895519226239 12.67716074852936 6 30.425326359573766 39.19773210039295 18.158743454168473 12.218198085864811 7 27.047582304120066 32.41915056108001 22.394025687771666 18.139241447028258 8 24.11839756653223 38.78734508223546 20.328435937285082 16.765821413947222 9 25.908962272354298 15.888677911865006 20.272028609960046 37.93033120582064 10 14.117461070271126 30.355386925934706 34.84277922693858 20.684372776855582 11 33.60651461472428 23.345142732476795 24.87419907137335 18.174143581425568 12 22.488380916247745 25.611780328179833 32.38784027316703 19.51199848240539 13 28.688207127353664 22.747744543352034 25.860567172059685 22.703481157234613 14 20.120079294067807 23.14450932843489 28.075095402651357 28.66031597484595 15 22.105782348951806 32.08328656592308 23.611245045947992 22.199686039177124 16 21.27883245834104 29.214941422150982 28.41045437946685 21.095771740041133 17 20.80852759009899 29.497993935964033 28.176584221492234 21.516894252444743 18 20.573333691653716 28.77133521240758 30.220635776287935 20.434695319650764 19 22.751710848096923 27.67756224104107 27.79849537055334 21.772231540308674 20 22.713986483816146 28.403430423269967 28.59692526577876 20.285657827135122 21 22.20607789226374 27.51557734600918 27.54024180657687 22.738102955150207 22 22.141477310451453 28.950713109130515 27.758930857975724 21.14887872244231 23 21.499855354492155 28.68992271345488 28.118138230519097 21.692083701533875 24 22.249763667002856 27.668507798198494 28.93948926779453 21.142239267004122 25 21.923025239311038 28.20194610660111 28.426277972318175 21.448750681769678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 185.0 1 185.0 2 199.5 3 214.0 4 214.0 5 214.0 6 391.0 7 568.0 8 568.0 9 568.0 10 943.0 11 1318.0 12 1318.0 13 1318.0 14 2290.0 15 3262.0 16 3262.0 17 3262.0 18 5823.5 19 8385.0 20 8385.0 21 8385.0 22 13144.0 23 17903.0 24 17903.0 25 17903.0 26 25326.0 27 32749.0 28 32749.0 29 32749.0 30 42818.5 31 52888.0 32 52888.0 33 52888.0 34 59608.0 35 66328.0 36 66328.0 37 66328.0 38 71458.0 39 76588.0 40 76588.0 41 76588.0 42 79344.0 43 82100.0 44 82100.0 45 82100.0 46 82025.5 47 81951.0 48 81951.0 49 81951.0 50 77870.5 51 73790.0 52 73790.0 53 73790.0 54 64087.0 55 54384.0 56 54384.0 57 54384.0 58 46130.0 59 37876.0 60 37876.0 61 37876.0 62 30229.5 63 22583.0 64 22583.0 65 22583.0 66 17051.5 67 11520.0 68 11520.0 69 11520.0 70 8343.0 71 5166.0 72 5166.0 73 5166.0 74 3522.5 75 1879.0 76 1879.0 77 1879.0 78 1279.0 79 679.0 80 679.0 81 679.0 82 441.5 83 204.0 84 204.0 85 204.0 86 127.0 87 50.0 88 50.0 89 50.0 90 30.0 91 10.0 92 10.0 93 10.0 94 6.0 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11160608427049773 2 0.11808745743634816 3 0.016598638595470627 4 0.0 5 0.005532879531823543 6 0.35078456231761257 7 0.022289600399631983 8 0.01090767679130927 9 0.011856170425336161 10 0.005058632714810096 11 0.005532879531823543 12 3.16164544675631E-4 13 7.904113616890775E-4 14 0.0 15 0.0030035631744184943 16 7.904113616890775E-4 17 0.0 18 1.580822723378155E-4 19 1.580822723378155E-4 20 0.0011065759063647086 21 0.01470165132741684 22 0.0 23 1.580822723378155E-4 24 0.0 25 0.005849044076499173 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 632582.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 84.24003050594602 #Duplication Level Percentage of deduplicated Percentage of total 1 91.79678770473504 77.3296419659473 2 6.424678112262666 10.824301603357814 3 0.9314714153798446 2.3540154134104454 4 0.31857803826954745 1.073480946494045 5 0.15740461127744546 0.6629884627894291 6 0.07064450280525711 0.3570657042835349 7 0.050189517906102946 0.2959576563642186 8 0.03759023856292587 0.2533282274613331 9 0.030325401034742065 0.22991514374445474 >10 0.1540040877485661 2.4246438075446446 >50 0.015007438025799893 0.8460822581765429 >100 0.011443026078232854 1.918598175584928 >500 0.0013131341396593868 0.8871600490661136 >1k 5.627717741397372E-4 0.5428205857752227 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1341 0.2119883272050106 No Hit GTCCTACAGTGGACATTTCTAAATT 1085 0.1715192654865298 No Hit CTGTAGGACGTGGAATATGGCAAGA 1009 0.15950501278885582 No Hit GTCCTAAAGTGTGTATTTCTCATTT 940 0.14859733599754657 No Hit CTTTAGGACGTGAAATATGGCGAGG 900 0.14227404510403394 No Hit GTCCTACAGTGTGCATTTCTCATTT 876 0.13848007056792638 No Hit GGTATCAACGCAGAGTACTTTTTTT 863 0.13642500102753477 No Hit CTGTAGGACCTGGAATATGGCGAGA 757 0.11966828015972633 No Hit TATCAACGCAGAGTACTTTTTTTTT 754 0.11919403334271289 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACTATC 40 0.005309753 14.235707 7 TAGGACC 300 0.0 13.960204 4 TAATAGT 85 2.6280395E-7 13.437631 4 GGTATCA 275 0.0 13.167211 1 GTAGGAC 685 0.0 12.922686 3 GGACCTG 405 0.0 12.691096 6 CAAGACA 60 4.0201578E-4 12.691095 4 TGTAGGA 735 0.0 12.575559 2 AGGACCT 440 0.0 12.546879 5 GGCGAGG 295 0.0 12.225981 19 CTGTAGG 720 0.0 12.17582 1 GTCATAC 55 0.0029960142 12.127694 1 GTGTTAG 65 7.810192E-4 11.7278805 1 GTCCTAG 90 7.19333E-6 11.646437 1 TGAGTTA 240 0.0 11.4649315 12 TGGCGAG 650 0.0 11.389465 18 CTAAAGT 295 0.0 11.292924 4 GGACTGT 85 5.20778E-5 11.198026 6 CTACTAC 60 0.005737858 11.117053 1 CTTAGAC 60 0.005785524 11.104709 3 >>END_MODULE