FastQCFastQC Report
Thu 26 May 2016
SRR1926856_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926856_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594683
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT10800.18160936162627822No Hit
GTCCTACAGTGTGCATTTCTCATTT8940.1503321937906414No Hit
GTCCTAAAGTGTGTATTTCTCATTT8670.14579195974998446No Hit
GTATCAACGCAGAGTACTTTTTTTT8450.14209250979093063No Hit
CTGTAGGACGTGGAATATGGCAAGA8370.1407472552603656No Hit
CTTTAGGACGTGAAATATGGCGAGG7900.1328438848932961No Hit
CTGTAGGACCTGGAATATGGCGAGA6190.10408906930246872No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAATC350.002196107516.25442713
ACGAAAT350.002196107516.25442712
AATCCCG851.7533239E-814.50149919
GTATTAG706.934215E-613.6290321
GGCGAGG1850.013.32570219
TAATACC655.3172153E-513.1862454
GCCCTAT603.9419514E-412.720431
GGTATAG603.9419514E-412.720431
AGGATAG604.0243455E-412.689295
CCCTTAC550.002968879212.1422291
GTCTTAT550.002968879212.1422291
TTACTCT550.003008640612.1206894
GTCCTAA3400.011.7851061
CTATAAG657.7308516E-411.7419361
ATAGGAC1302.4374458E-911.7399493
CTTAGAC657.741841E-411.7399493
CTTACTC657.741841E-411.7399493
CTAGGGT657.8469235E-411.7211074
TAGGACT1552.3646862E-1111.6738444
TAAAACC907.237697E-611.6397094
Produced by FastQC (version 0.11.5)