##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926856_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594683 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.052690256825905 31.0 28.0 31.0 16.0 34.0 2 28.19604898744373 31.0 28.0 31.0 16.0 34.0 3 27.68931010302968 31.0 28.0 31.0 16.0 34.0 4 31.266526872300034 35.0 32.0 37.0 17.0 37.0 5 31.633389553762257 35.0 32.0 37.0 18.0 37.0 6 30.85876845310863 35.0 31.0 37.0 11.0 37.0 7 30.939983150687006 35.0 32.0 37.0 11.0 37.0 8 30.830956997257363 35.0 32.0 37.0 11.0 37.0 9 32.860998212493044 37.0 33.0 39.0 17.0 39.0 10 33.02696058236069 37.0 33.0 39.0 17.0 39.0 11 33.197293347884504 38.0 33.0 39.0 17.0 39.0 12 33.070491337401606 38.0 33.0 39.0 16.0 39.0 13 33.182305530845845 38.0 33.0 39.0 17.0 39.0 14 34.173374722331054 39.0 34.0 41.0 17.0 41.0 15 33.76483437394376 39.0 33.0 41.0 10.0 41.0 16 33.841275099506795 39.0 33.0 41.0 11.0 41.0 17 34.062218022038635 39.0 34.0 41.0 16.0 41.0 18 34.110700995320194 39.0 34.0 41.0 11.0 41.0 19 33.485515812626225 39.0 33.0 40.0 10.0 41.0 20 33.761368661959395 39.0 33.0 41.0 10.0 41.0 21 33.7493807625239 39.0 34.0 41.0 7.0 41.0 22 33.744919562186915 39.0 34.0 41.0 2.0 41.0 23 33.73426010160035 39.0 34.0 41.0 2.0 41.0 24 32.439625817452324 38.0 32.0 40.0 2.0 41.0 25 33.003010007012136 39.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.7291828883704383 1101 2 -0.9354314405218105 1101 3 -1.5660691622701108 1101 4 -1.2827876371739997 1101 5 -0.939870433279232 1101 6 -2.7058179887229237 1101 7 -1.202519407149616 1101 8 -0.5503906138894976 1101 9 -0.8518153706331013 1101 10 -9.054780461682814 1101 11 -4.440746159263266 1101 12 -2.1594970134319524 1101 13 -1.5306622815754274 1101 14 -1.0820339034037971 1101 15 -2.624560638831877 1101 16 -1.1416757762157346 1101 17 -0.968563111250198 1101 18 -0.8277852704586905 1101 19 -1.5877014082569687 1101 20 -0.8253122283999801 1101 21 -0.7821606933465475 1101 22 -0.7941663325820727 1101 23 -0.931129750404736 1101 24 -0.7503832517070101 1101 25 -0.61255388655632 1102 1 0.10773426753301862 1102 2 -0.1408782767905663 1102 3 -0.36943119749515674 1102 4 -0.5091738080807175 1102 5 -0.044295743129200815 1102 6 -0.8143033116192058 1102 7 0.12854517210674743 1102 8 0.6396354787393328 1102 9 0.5367312699409368 1102 10 0.44683858593623427 1102 11 0.29949911144775854 1102 12 0.20837121879244336 1102 13 0.11573693564570675 1102 14 -0.12995693014874377 1102 15 0.6955646057865152 1102 16 0.2728173744691915 1102 17 0.05211007857367633 1102 18 -0.10176570098706605 1102 19 0.4110317946132227 1102 20 0.2911809222849513 1102 21 0.3183794240702795 1102 22 0.46862818796586936 1102 23 -0.09136588869566964 1102 24 -3.1244354369646743 1102 25 -1.8349948519835877 1103 1 0.07613770518503671 1103 2 -0.0752385816389527 1103 3 0.13167079699162798 1103 4 -0.25731715168376823 1103 5 0.03272971495414723 1103 6 0.6694638361025369 1103 7 0.4315242543185178 1103 8 0.5536228307743585 1103 9 0.3707821861076326 1103 10 0.501878637825321 1103 11 0.245323012858492 1103 12 -0.08846808719101773 1103 13 0.12881947172483876 1103 14 0.07195388548284853 1103 15 0.3362248944195656 1103 16 0.3141613018244698 1103 17 0.060443465958144316 1103 18 -0.0903682630107383 1103 19 -1.8901506279585334 1103 20 -0.4480790096106233 1103 21 -0.26668170776043354 1103 22 -0.028815625882032236 1103 23 0.07685334269722688 1103 24 1.5293878158324716 1103 25 0.8723260495892973 1104 1 -0.4649522224475895 1104 2 -0.17736825739819295 1104 3 -1.5699896978183645 1104 4 -0.781992166193195 1104 5 -0.41161571727270285 1104 6 -1.7721106807206937 1104 7 -0.3953384736592547 1104 8 0.143972906017936 1104 9 -0.15704894983928597 1104 10 -0.16036115549880492 1104 11 -0.39291459249535876 1104 12 -0.4356029247111124 1104 13 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0.8504868061336595 2215 2 0.2911150463419965 2215 3 0.7486425262494514 2215 4 1.1614897056207703 2215 5 1.0622659483438852 2215 6 1.6862202660689292 2215 7 1.1354479402247044 2215 8 -0.09001815876240471 2215 9 0.548647252692362 2215 10 0.4044048677556802 2215 11 0.3821297972176865 2215 12 0.17293708330767288 2215 13 0.5389613062118528 2215 14 0.31410037963008364 2215 15 0.3904851788843047 2215 16 0.37058563152859136 2215 17 0.02321370912034837 2215 18 -0.7589786652380042 2215 19 0.2987402380684969 2215 20 -3.704238746653708 2215 21 -2.2289601224591387 2215 22 -0.8795860281977141 2215 23 -0.641743409908635 2215 24 -0.392403721844353 2215 25 -0.10231770634441517 2216 1 -0.6873829266500415 2216 2 -1.065657290192334 2216 3 -0.1841685798732584 2216 4 -0.4224330039746462 2216 5 -0.5767063504859173 2216 6 -1.5635955842168698 2216 7 -10.13951527842077 2216 8 -10.89308194355893 2216 9 -5.6295270705209965 2216 10 -3.1680539147766282 2216 11 -2.1543600274173684 2216 12 -1.7847191718548352 2216 13 -1.8325721604478211 2216 14 -1.963756759062342 2216 15 -3.071475029229166 2216 16 -1.8641007892034587 2216 17 -2.2482229696156573 2216 18 -1.8863257237985849 2216 19 -1.3604914446225962 2216 20 -2.08616039508032 2216 21 -4.894483991954811 2216 22 -3.3471562431718525 2216 23 -3.1815945741818403 2216 24 -3.0674956023481954 2216 25 -2.6284057444843008 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37726.0 3 3.0 4 242.0 5 1525.0 6 2059.0 7 1649.0 8 1404.0 9 1244.0 10 1187.0 11 1148.0 12 1106.0 13 1110.0 14 1072.0 15 1173.0 16 1244.0 17 1430.0 18 1582.0 19 1908.0 20 2496.0 21 3228.0 22 4080.0 23 4792.0 24 5506.0 25 6224.0 26 7197.0 27 7811.0 28 8777.0 29 10386.0 30 12585.0 31 15352.0 32 19592.0 33 25338.0 34 34175.0 35 47557.0 36 68477.0 37 107407.0 38 144891.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.93516819262652 18.854999266932595 12.763461898189576 25.44637064225131 2 13.88589084112779 20.190939628391746 42.44197304282672 23.48119648765375 3 18.717217759113357 25.431862612206825 29.40269178505458 26.44822784362524 4 12.127132534337631 16.977480706643377 39.029290899728394 31.866095859290596 5 12.509671472687762 38.25109497890778 36.532432232411374 12.706801315993083 6 30.62750612233914 37.49024462445168 19.563396485373666 12.318852767835518 7 27.828602270235624 31.384911197692656 22.853817740221626 17.93266879185009 8 24.00524844966666 38.45703675095331 21.156210524895563 16.381504274484474 9 26.098342457804574 15.85505125682233 20.39340346982197 37.653202815551126 10 14.362807191076257 29.685478729399335 35.06650584817456 20.88520823134985 11 33.747075632428654 22.932760148879954 24.88458103546579 18.43558318322561 12 22.850967926400592 25.099483660460503 32.378021090872394 19.671527322266513 13 28.66996200660301 22.501879487433925 25.803722646520132 23.02443585944293 14 20.368772568945133 22.765474706803438 27.951938133128817 28.913814591122616 15 22.785419000238825 31.651311341412956 23.33740341954516 22.225866238803057 16 21.469321851453174 28.55102933261572 28.639666307857915 21.339982508073195 17 21.20663208670555 29.07878714486582 28.212269831338592 21.502310937090037 18 21.036592031726435 28.14089874752558 30.178797577461854 20.643711643286135 19 23.491438759376997 27.17176304369765 27.461735122952195 21.875063073973156 20 23.022649944245515 28.027098529864393 28.417464020276807 20.53278750561329 21 22.599872515695875 27.150793076828634 27.317128138544156 22.932206268931335 22 22.479867871342748 28.60460970244142 27.626427076956194 21.289095349259636 23 21.955268362912232 28.248240739494896 27.854341303265564 21.942149594327308 24 23.10128391855846 27.500479325110245 28.24217186412058 21.156064892210715 25 22.567038467230873 27.63091505994107 28.232848512573494 21.569197960254566 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 196.0 1 196.0 2 257.0 3 318.0 4 318.0 5 318.0 6 485.0 7 652.0 8 652.0 9 652.0 10 910.0 11 1168.0 12 1168.0 13 1168.0 14 2040.5 15 2913.0 16 2913.0 17 2913.0 18 5109.5 19 7306.0 20 7306.0 21 7306.0 22 11616.5 23 15927.0 24 15927.0 25 15927.0 26 22705.5 27 29484.0 28 29484.0 29 29484.0 30 38746.5 31 48009.0 32 48009.0 33 48009.0 34 54368.5 35 60728.0 36 60728.0 37 60728.0 38 65466.0 39 70204.0 40 70204.0 41 70204.0 42 73469.5 43 76735.0 44 76735.0 45 76735.0 46 77567.0 47 78399.0 48 78399.0 49 78399.0 50 74488.0 51 70577.0 52 70577.0 53 70577.0 54 61810.0 55 53043.0 56 53043.0 57 53043.0 58 44601.0 59 36159.0 60 36159.0 61 36159.0 62 29186.0 63 22213.0 64 22213.0 65 22213.0 66 17027.5 67 11842.0 68 11842.0 69 11842.0 70 8668.0 71 5494.0 72 5494.0 73 5494.0 74 3833.5 75 2173.0 76 2173.0 77 2173.0 78 1469.0 79 765.0 80 765.0 81 765.0 82 514.0 83 263.0 84 263.0 85 263.0 86 175.0 87 87.0 88 87.0 89 87.0 90 53.0 91 19.0 92 19.0 93 19.0 94 12.0 95 5.0 96 5.0 97 5.0 98 4.5 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21624966578832755 2 0.13082600309744857 3 0.13486176668914363 4 0.07348452873211442 5 0.02539167926441482 6 0.023373797468567287 7 0.30150517166288593 8 0.2936018012958164 9 0.02152407248904038 10 0.03766712685582066 11 0.018497249795269077 12 0.01900172024423096 13 0.017824622529986563 14 0.019506190693192843 15 0.016983838448383425 16 0.020683288407437238 17 0.020010661142154728 18 0.016815681632062797 19 0.024046424733849797 20 0.01883356342791033 21 0.016815681632062797 22 0.018665406611589705 23 0.019338033876872215 24 0.016311211183100912 25 0.01664752481574217 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 594683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.3092481677382 #Duplication Level Percentage of deduplicated Percentage of total 1 93.8969749491322 81.04177313084547 2 4.545659014031514 7.8466482385592435 3 0.7624791337036333 1.9742700232064678 4 0.2679769155946763 0.925155444451438 5 0.15075380984334452 0.6505723993000618 6 0.07432597262411018 0.38490112899137047 7 0.05681783789632073 0.34327334099435064 8 0.03974767150755059 0.27444733153879364 9 0.027518141495081888 0.21375630930725611 >10 0.15123509184819978 2.434097327926375 >50 0.01286589529762597 0.7752920962546114 >100 0.012281010323231797 2.1377638632533347 >500 0.0011696200307125025 0.8163411194844863 >1k 1.9493667178541705E-4 0.18170824588690132 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1080 0.18160936162627822 No Hit GTCCTACAGTGTGCATTTCTCATTT 894 0.1503321937906414 No Hit GTCCTAAAGTGTGTATTTCTCATTT 867 0.14579195974998446 No Hit GTATCAACGCAGAGTACTTTTTTTT 845 0.14209250979093063 No Hit CTGTAGGACGTGGAATATGGCAAGA 837 0.1407472552603656 No Hit CTTTAGGACGTGAAATATGGCGAGG 790 0.1328438848932961 No Hit CTGTAGGACCTGGAATATGGCGAGA 619 0.10408906930246872 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAAATC 35 0.0021961075 16.254427 13 ACGAAAT 35 0.0021961075 16.254427 12 AATCCCG 85 1.7533239E-8 14.501499 19 GTATTAG 70 6.934215E-6 13.629032 1 GGCGAGG 185 0.0 13.325702 19 TAATACC 65 5.3172153E-5 13.186245 4 GCCCTAT 60 3.9419514E-4 12.72043 1 GGTATAG 60 3.9419514E-4 12.72043 1 AGGATAG 60 4.0243455E-4 12.68929 5 CCCTTAC 55 0.0029688792 12.142229 1 GTCTTAT 55 0.0029688792 12.142229 1 TTACTCT 55 0.0030086406 12.120689 4 GTCCTAA 340 0.0 11.785106 1 CTATAAG 65 7.7308516E-4 11.741936 1 ATAGGAC 130 2.4374458E-9 11.739949 3 CTTAGAC 65 7.741841E-4 11.739949 3 CTTACTC 65 7.741841E-4 11.739949 3 CTAGGGT 65 7.8469235E-4 11.721107 4 TAGGACT 155 2.3646862E-11 11.673844 4 TAAAACC 90 7.237697E-6 11.639709 4 >>END_MODULE