FastQCFastQC Report
Thu 26 May 2016
SRR1926856_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926856_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences594683
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13790.23188824970614597No Hit
GTCCTACAGTGGACATTTCTAAATT11350.19085798652391273No Hit
CTGTAGGACGTGGAATATGGCAAGA9990.16798865950430733No Hit
CTTTAGGACGTGAAATATGGCGAGG9760.16412105272893288No Hit
GTCCTAAAGTGTGTATTTCTCATTT9050.15218191877016832No Hit
GGTATCAACGCAGAGTACTTTTTTT8710.14646458701526696No Hit
GTCCTACAGTGTGCATTTCTCATTT8430.14175619615828938No Hit
CTGTAGGACCTGGAATATGGCGAGA7520.12645392587311224No Hit
TATCAACGCAGAGTACTTTTTTTTT7340.12342710317934091No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG250.00595409919.0404975
TAGGTTG707.0868464E-613.6003545
TAGGACC3200.013.3878484
TTATGCT500.001473801713.3283484
ACGTTTT500.001510748913.28463719
GTCCTAG603.978513E-412.7065291
CTATTAG603.978513E-412.7065291
AGCCTAT604.012669E-412.6936656
TCCAACG604.1254517E-412.65203518
TGTAGGA6750.012.4241612
GTAGGAC6550.012.354523
CTGTAGG6400.012.0612751
TAGGACT1209.649739E-911.90031054
GGACATG1459.276846E-1111.8182396
GGCGAGG2900.011.77948219
GTATTGA657.8021566E-411.7291021
TATTAGG657.8021566E-411.7291022
GTGTAGG657.8021566E-411.7291021
ATTATAC657.868767E-411.7172283
CTAGACA1001.8741448E-611.4242984
Produced by FastQC (version 0.11.5)