##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926856_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 594683 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.93648212577121 31.0 30.0 33.0 26.0 34.0 2 30.255120795449002 31.0 30.0 34.0 26.0 34.0 3 30.303467561709347 31.0 30.0 34.0 26.0 34.0 4 33.8883304214178 37.0 35.0 37.0 30.0 37.0 5 33.855960234276075 37.0 35.0 37.0 30.0 37.0 6 33.7793479887604 37.0 35.0 37.0 29.0 37.0 7 33.77885865242491 37.0 35.0 37.0 29.0 37.0 8 33.8089402252965 37.0 35.0 37.0 30.0 37.0 9 35.369423709774786 39.0 35.0 39.0 30.0 39.0 10 35.28792146404051 39.0 35.0 39.0 30.0 39.0 11 35.296225047630415 39.0 35.0 39.0 30.0 39.0 12 35.202312828851674 39.0 35.0 39.0 29.0 39.0 13 35.213421268137814 39.0 35.0 39.0 29.0 39.0 14 36.31430358695305 40.0 36.0 41.0 29.0 41.0 15 36.285792598745886 40.0 36.0 41.0 29.0 41.0 16 36.2652942828364 40.0 36.0 41.0 29.0 41.0 17 36.24173046816539 40.0 36.0 41.0 29.0 41.0 18 36.21627993401527 40.0 36.0 41.0 29.0 41.0 19 36.29428956267457 40.0 37.0 41.0 29.0 41.0 20 36.25479120808902 40.0 37.0 41.0 29.0 41.0 21 36.20089526689009 40.0 37.0 41.0 29.0 41.0 22 36.120089190375374 40.0 37.0 41.0 29.0 41.0 23 36.04898071745787 40.0 37.0 41.0 29.0 41.0 24 35.966536793552194 40.0 36.0 41.0 28.0 41.0 25 35.86222575725218 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1413097929284497 1101 2 0.15798133848443996 1101 3 0.4708178102611136 1101 4 0.3765756987973248 1101 5 0.07057596488988338 1101 6 0.2062925254547494 1101 7 0.5076893031842076 1101 8 0.4172018449028556 1101 9 0.4271760102285853 1101 10 0.43819830108459 1101 11 0.4433592110186453 1101 12 0.35179856639547324 1101 13 0.5667586872589396 1101 14 0.4402503785876277 1101 15 0.3331560885662981 1101 16 0.35588808577658426 1101 17 0.4491547604321937 1101 18 0.503349716205534 1101 19 0.28398780297446535 1101 20 0.5794574274753543 1101 21 0.5482402047149151 1101 22 0.2188468251892246 1101 23 0.32406485375077665 1101 24 0.4425016117364535 1101 25 0.5748027623935101 1102 1 -0.46317292070494887 1102 2 -0.35694234057622864 1102 3 -0.19570012842120832 1102 4 -0.45850714529922243 1102 5 -0.34204764893920725 1102 6 -0.2393787074219631 1102 7 -0.25649334521762057 1102 8 -0.35964094700842963 1102 9 -0.2507274470447385 1102 10 -0.011213310135239851 1102 11 -0.14205240020118026 1102 12 -0.09553346230642035 1102 13 -0.026613785017644886 1102 14 -0.29141563576332175 1102 15 -0.22839250895490437 1102 16 -0.13365137932452598 1102 17 -0.004863504407985886 1102 18 -0.07064115137437454 1102 19 -0.22502589565566922 1102 20 0.11188795585108124 1102 21 -0.12858432235618267 1102 22 -0.04016687344090997 1102 23 -0.13029913842143515 1102 24 -0.0929700125296904 1102 25 0.12962859409605443 1103 1 -0.7967017029329355 1103 2 -0.7196678148749136 1103 3 -0.576837148041772 1103 4 -0.6796247065121293 1103 5 -0.7430127859588254 1103 6 -0.7905160513493144 1103 7 -0.9346499853992327 1103 8 -0.6728240181937721 1103 9 -0.8068448461042479 1103 10 -0.6762446057408837 1103 11 -0.7164866469986535 1103 12 -0.7275684874401946 1103 13 -0.6670613283936788 1103 14 -0.6573219110997925 1103 15 -0.6875305014958499 1103 16 -0.6398722322513919 1103 17 -0.4778260469732558 1103 18 -0.36814852935393816 1103 19 -0.4958817412856362 1103 20 -0.43573390242684695 1103 21 -0.2620806739047481 1103 22 -0.39047788365657254 1103 23 -0.43547831792524505 1103 24 -0.3921313551598189 1103 25 -0.35523471059018163 1104 1 -0.33789948825504723 1104 2 -0.36298500379536236 1104 3 0.006593342813438596 1104 4 -0.1702796081305067 1104 5 -0.2208660070516828 1104 6 -0.15264567574775612 1104 7 0.0012170620221994 1104 8 -0.1439977666593677 1104 9 -0.343950459326571 1104 10 -0.1626147322425453 1104 11 -0.08844929742161156 1104 12 0.0241110108674647 1104 13 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9 0.37000305157869917 1107 10 0.35965362164818515 1107 11 0.31021067719251505 1107 12 0.5928054792659267 1107 13 0.5010766401888418 1107 14 0.4745653981920128 1107 15 0.5115193907085995 1107 16 0.43953675160236827 1107 17 0.45710651700528615 1107 18 0.41238240322947917 1107 19 0.3037364169781611 1107 20 0.47469370684527235 1107 21 0.4677741970687137 1107 22 0.3954476875869304 1107 23 0.32403552049566287 1107 24 0.2767467204161633 1107 25 0.3752192946160875 1108 1 0.5886299922172711 1108 2 0.6370248115774757 1108 3 0.629545610655331 1108 4 0.5035653678709053 1108 5 0.6964983690341455 1108 6 0.6024607719706623 1108 7 0.6176151146522244 1108 8 0.640544984197021 1108 9 0.4397727078979372 1108 10 0.5193451032518084 1108 11 0.566805362426642 1108 12 0.7586468909628721 1108 13 0.35118602905114926 1108 14 0.5319608749277194 1108 15 0.5287429109397337 1108 16 0.670382088292456 1108 17 0.5383469077395162 1108 18 0.5967688269336549 1108 19 0.6221671629126604 1108 20 0.5151095238709189 1108 21 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25 -0.13831893940836437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14160.0 3 12.0 4 243.0 5 1483.0 6 2045.0 7 1523.0 8 1175.0 9 1061.0 10 905.0 11 887.0 12 748.0 13 756.0 14 745.0 15 694.0 16 745.0 17 786.0 18 869.0 19 1097.0 20 1330.0 21 1818.0 22 2298.0 23 2743.0 24 3090.0 25 3801.0 26 4699.0 27 5065.0 28 6046.0 29 7212.0 30 9121.0 31 11986.0 32 15715.0 33 20797.0 34 28105.0 35 39022.0 36 58353.0 37 101484.0 38 242064.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.460166860821374 19.653619021325593 10.787875319008531 28.098338798844498 2 13.75327005888631 21.09907647140024 40.67246214812146 24.475191321591986 3 18.255759585854747 25.22158722043009 29.651649752595976 26.87100344111919 4 11.84664770978824 17.766608428355948 38.404494495386615 31.982249366469194 5 12.628309476403162 38.85237851800032 35.613867513318624 12.905444492277887 6 31.11320318602764 38.34435890557487 18.022719258730614 12.519718649666869 7 27.522941952151562 32.020842864447374 22.111567857046747 18.344647326354316 8 24.600833481889094 38.47487613813331 20.047963889178526 16.876326490799073 9 26.12695337463758 15.824100421787653 20.03625893860198 38.01268726497279 10 14.535486963028985 30.01875036786036 34.32444022164112 21.12132244746954 11 33.723476172698746 23.046006585342063 24.69906870213603 18.53144853982316 12 23.031041904890024 25.239960987421806 31.958868635232395 19.770128472455774 13 28.8308967205782 22.460894803574373 25.78117905824665 22.927029417600785 14 20.442151532833456 22.972070834377305 27.66246891200858 28.92330872078065 15 22.465438756686492 31.702341975115566 23.442957342656168 22.389261925541774 16 21.465289794024024 28.772736979994317 28.26742404751455 21.49454917846711 17 21.02787708388685 29.22452672184462 28.176571680326628 21.571024513941907 18 20.899076305288418 28.322097542027013 29.990061831885207 20.78876432079936 19 23.058917301881177 27.19424363650428 27.755384819760508 21.991454241854036 20 23.130035296471867 27.9703099329552 28.249453064436658 20.650201706136272 21 22.474453585916006 27.16715662861333 27.482156191347517 22.876233594123146 22 22.518589767304206 28.648252343269178 27.387242257206374 21.445915632220245 23 21.8602547243737 28.397361951429506 27.743903464372554 21.99847985982424 24 22.57122293931883 27.39598642635896 28.64287131609835 21.389919318223857 25 22.29657122004295 27.921122421351967 28.190359593907605 21.59194676469747 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 201.0 1 201.0 2 201.0 3 201.0 4 201.0 5 201.0 6 377.0 7 553.0 8 553.0 9 553.0 10 876.0 11 1199.0 12 1199.0 13 1199.0 14 1905.0 15 2611.0 16 2611.0 17 2611.0 18 4781.0 19 6951.0 20 6951.0 21 6951.0 22 11143.5 23 15336.0 24 15336.0 25 15336.0 26 22129.0 27 28922.0 28 28922.0 29 28922.0 30 38391.0 31 47860.0 32 47860.0 33 47860.0 34 53807.0 35 59754.0 36 59754.0 37 59754.0 38 64505.5 39 69257.0 40 69257.0 41 69257.0 42 72556.5 43 75856.0 44 75856.0 45 75856.0 46 77454.0 47 79052.0 48 79052.0 49 79052.0 50 75600.0 51 72148.0 52 72148.0 53 72148.0 54 63092.5 55 54037.0 56 54037.0 57 54037.0 58 45439.0 59 36841.0 60 36841.0 61 36841.0 62 29905.0 63 22969.0 64 22969.0 65 22969.0 66 17508.5 67 12048.0 68 12048.0 69 12048.0 70 8794.5 71 5541.0 72 5541.0 73 5541.0 74 3886.0 75 2231.0 76 2231.0 77 2231.0 78 1498.0 79 765.0 80 765.0 81 765.0 82 516.0 83 267.0 84 267.0 85 267.0 86 164.5 87 62.0 88 62.0 89 62.0 90 40.0 91 18.0 92 18.0 93 18.0 94 10.5 95 3.0 96 3.0 97 3.0 98 1.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11014271469001133 2 0.11047902832265258 3 0.01832909297894845 4 0.0 5 0.004540234040656955 6 0.33126892815163705 7 0.023373797468567287 8 0.01160282032612333 9 0.011939133958764586 10 0.0047083908569775824 11 0.004876547673298211 12 5.044704489618839E-4 13 8.407840816031397E-4 14 0.0 15 0.0023541954284887912 16 3.3631363264125593E-4 17 1.6815681632062796E-4 18 0.0010089408979237677 19 3.3631363264125593E-4 20 0.0010089408979237677 21 0.01328438848932961 22 1.6815681632062796E-4 23 1.6815681632062796E-4 24 1.6815681632062796E-4 25 0.006726272652825118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 594683.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.37934144619635 #Duplication Level Percentage of deduplicated Percentage of total 1 92.07937492317107 76.77517641871411 2 5.97968553958264 9.971644846914876 3 0.9683819644672957 2.4222915139697108 4 0.34491126675132466 1.1503389711639525 5 0.17460273154579964 0.7279130385497893 6 0.09879644984247257 0.494254975505252 7 0.06683652031652831 0.39009495339832173 8 0.04687955095989745 0.31270288690637205 9 0.030957433507947878 0.23230893768813954 >10 0.17725138937721457 2.7074776064450634 >50 0.016565199053675726 0.9319686960293996 >100 0.01353488596700005 2.2611022519620048 >500 0.001818119010356997 1.199273542412402 >1k 4.0402644674599934E-4 0.42345136034055875 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1379 0.23188824970614597 No Hit GTCCTACAGTGGACATTTCTAAATT 1135 0.19085798652391273 No Hit CTGTAGGACGTGGAATATGGCAAGA 999 0.16798865950430733 No Hit CTTTAGGACGTGAAATATGGCGAGG 976 0.16412105272893288 No Hit GTCCTAAAGTGTGTATTTCTCATTT 905 0.15218191877016832 No Hit GGTATCAACGCAGAGTACTTTTTTT 871 0.14646458701526696 No Hit GTCCTACAGTGTGCATTTCTCATTT 843 0.14175619615828938 No Hit CTGTAGGACCTGGAATATGGCGAGA 752 0.12645392587311224 No Hit TATCAACGCAGAGTACTTTTTTTTT 734 0.12342710317934091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGACCG 25 0.005954099 19.040497 5 TAGGTTG 70 7.0868464E-6 13.600354 5 TAGGACC 320 0.0 13.387848 4 TTATGCT 50 0.0014738017 13.328348 4 ACGTTTT 50 0.0015107489 13.284637 19 GTCCTAG 60 3.978513E-4 12.706529 1 CTATTAG 60 3.978513E-4 12.706529 1 AGCCTAT 60 4.012669E-4 12.693665 6 TCCAACG 60 4.1254517E-4 12.652035 18 TGTAGGA 675 0.0 12.424161 2 GTAGGAC 655 0.0 12.35452 3 CTGTAGG 640 0.0 12.061275 1 TAGGACT 120 9.649739E-9 11.9003105 4 GGACATG 145 9.276846E-11 11.818239 6 GGCGAGG 290 0.0 11.779482 19 GTATTGA 65 7.8021566E-4 11.729102 1 TATTAGG 65 7.8021566E-4 11.729102 2 GTGTAGG 65 7.8021566E-4 11.729102 1 ATTATAC 65 7.868767E-4 11.717228 3 CTAGACA 100 1.8741448E-6 11.424298 4 >>END_MODULE