FastQCFastQC Report
Thu 26 May 2016
SRR1926855_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926855_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649850
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT11110.1709625298145726No Hit
GTCCTACAGTGTGCATTTCTCATTT9230.1420327767946449No Hit
CTGTAGGACGTGGAATATGGCAAGA9110.14018619681464953No Hit
GTCCTAAAGTGTGTATTTCTCATTT7870.12110487035469723No Hit
GTATCAACGCAGAGTACTTTTTTTT7600.11695006539970763No Hit
CTTTAGGACGTGAAATATGGCGAGG7060.1086404554897284No Hit
CTGTAGGACCTGGAATATGGCGAGA6670.10263907055474339No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCACC508.527693E-515.2359824
GGGTAAT500.001452690713.3541881
TAATTGT500.001471445413.3314854
ATCCTAC701.04843486E-412.26405051
TAGGACC2500.012.1887864
GCCCTAG550.002973168912.140171
GGACTCC550.003016552212.1167216
AGGACGT5150.012.0158895
GTCCTAG802.7437023E-511.9233831
TAGACTG802.7985589E-511.9003525
GTCCTAA3300.011.8511191
TAAGACA1459.276846E-1111.821024
GCCTAGG657.7435724E-411.7399451
CCTAATA657.8193645E-411.726332
GTAATAC657.8193645E-411.726333
GGACGTG5150.011.646176
TGTAGGA6900.011.5988682
GACGTGG2900.011.4899957
CATCTAG751.993509E-411.4464461
AACCATC752.0296485E-411.4243377
Produced by FastQC (version 0.11.5)