##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926855_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 649850 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.837662537508656 31.0 28.0 31.0 16.0 34.0 2 27.965493575440487 31.0 28.0 31.0 16.0 34.0 3 27.455252750634763 31.0 27.0 31.0 10.0 34.0 4 31.030177733323075 35.0 32.0 37.0 16.0 37.0 5 31.401554204816495 35.0 32.0 37.0 17.0 37.0 6 30.585950603985534 35.0 30.0 37.0 11.0 37.0 7 30.677964145572055 35.0 31.0 37.0 11.0 37.0 8 30.572999923059168 35.0 31.0 37.0 10.0 37.0 9 32.59734861891206 37.0 32.0 39.0 15.0 39.0 10 32.78003847041625 37.0 33.0 39.0 15.0 39.0 11 32.97029622220512 37.0 33.0 39.0 16.0 39.0 12 32.835603600830964 37.0 33.0 39.0 11.0 39.0 13 32.93610987150881 38.0 33.0 39.0 11.0 39.0 14 33.921404939601445 39.0 33.0 40.0 11.0 41.0 15 33.49005001154112 39.0 33.0 40.0 10.0 41.0 16 33.579169039009 39.0 33.0 40.0 10.0 41.0 17 33.78892821420328 39.0 33.0 40.0 10.0 41.0 18 33.830408555820576 39.0 33.0 41.0 10.0 41.0 19 33.192510579364466 39.0 32.0 40.0 8.0 41.0 20 33.481986612295145 39.0 33.0 40.0 2.0 41.0 21 33.473183042240514 39.0 33.0 40.0 2.0 41.0 22 33.485942909902285 39.0 33.0 40.0 2.0 41.0 23 33.457605601292606 39.0 34.0 41.0 2.0 41.0 24 32.14870354697238 38.0 31.0 40.0 2.0 41.0 25 32.70118027237055 39.0 32.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.601092209194718 1101 2 -0.7201978038863643 1101 3 -1.5168268534871352 1101 4 -1.0313054221754676 1101 5 -0.7478435732073692 1101 6 -2.760976816393619 1101 7 -1.3872417452618961 1101 8 -0.5388608132602499 1101 9 -0.8425836625927232 1101 10 -9.258406915589937 1101 11 -4.575695418951341 1101 12 -2.104632426318698 1101 13 -1.3635075570805384 1101 14 -1.23890595766958 1101 15 -2.5389655710338346 1101 16 -0.8898973539800465 1101 17 -0.6804629193046026 1101 18 -0.44280930099034777 1101 19 -1.2646840859270938 1101 20 -0.44152454474487257 1101 21 -0.5459926460234925 1101 22 -0.44791767088860013 1101 23 -0.37583484444734694 1101 24 -0.3941405080504836 1101 25 -0.2508169110723344 1102 1 -0.44333583414894306 1102 2 -0.5112517254549935 1102 3 -0.824982501145179 1102 4 -0.9138050476623363 1102 5 -0.41640881339512603 1102 6 -1.2908099083740439 1102 7 -0.5232711570266027 1102 8 -0.0060212130738257486 1102 9 -0.16956587738016538 1102 10 -0.14994479135144445 1102 11 -0.3328975145603792 1102 12 -0.4055277207692427 1102 13 -0.3930243935264883 1102 14 -0.5061970209539766 1102 15 -0.10913060648774575 1102 16 -0.19074479397472288 1102 17 -0.32201535946572335 1102 18 -0.5358185972385598 1102 19 -0.021759263196642564 1102 20 -0.1846158177566224 1102 21 -0.39016189266951784 1102 22 -0.49861426531584385 1102 23 -0.8325858597940652 1102 24 -3.9257473358410238 1102 25 -2.1214964859583176 1103 1 -0.29218489834132555 1103 2 -0.7124246417514186 1103 3 -0.49464537344945114 1103 4 -0.7050589955763726 1103 5 -0.48873241607518736 1103 6 -0.1243723999949573 1103 7 -0.21031576338878466 1103 8 0.0824307681320775 1103 9 0.12338883034168902 1103 10 -0.19150818089871535 1103 11 -0.4049467508553093 1103 12 -0.3692864155335229 1103 13 -0.305729116057897 1103 14 -0.2829531996537966 1103 15 -0.08078045262432454 1103 16 0.031750755366019234 1103 17 -0.5396658478690668 1103 18 -0.4251585278363379 1103 19 -2.1055875472531476 1103 20 -0.9502133908612294 1103 21 -0.3667378625391038 1103 22 -0.4923537232551638 1103 23 -0.29239897887219257 1103 24 1.0048317331215912 1103 25 0.48580415412598654 1104 1 -0.4591628348056638 1104 2 -0.29134679378535466 1104 3 -1.5744555599061627 1104 4 -0.829209869878305 1104 5 -0.4817776722624352 1104 6 -2.122892342623661 1104 7 -0.5653983109184608 1104 8 0.15418455982544543 1104 9 -0.1430092898328681 1104 10 -0.11685225609173244 1104 11 -0.1091366864651846 1104 12 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0.38426523221067654 2215 4 0.9991290223364082 2215 5 1.0001469360027144 2215 6 1.4925708499797032 2215 7 0.8336543036562603 2215 8 -0.44298088578779016 2215 9 0.09152609220956975 2215 10 -0.05750240765438974 2215 11 0.021005753931248705 2215 12 -0.21836732905896383 2215 13 0.21086067191227187 2215 14 0.22820344815082905 2215 15 0.2996284113046457 2215 16 0.3659414543226873 2215 17 -0.17565019360436196 2215 18 -0.7519038826672855 2215 19 0.3089596263297594 2215 20 -3.8498874024513015 2215 21 -1.8846568040667648 2215 22 -1.0508104272385523 2215 23 -0.7855443170295118 2215 24 -0.5477247795887337 2215 25 -0.4860050957596229 2216 1 -0.6116244133417439 2216 2 -1.2087066386523624 2216 3 -0.09806703280130336 2216 4 -0.39811670325505233 2216 5 -0.7121646364602903 2216 6 -1.7121227556363081 2216 7 -10.095157218041265 2216 8 -11.220962324490493 2216 9 -6.062652715493485 2216 10 -3.4382785534492584 2216 11 -2.3708830903319367 2216 12 -2.1228536497835897 2216 13 -1.9494010084161495 2216 14 -1.94629862335033 2216 15 -3.0530245567688077 2216 16 -2.179475680689233 2216 17 -2.27775799432694 2216 18 -2.1849325332669025 2216 19 -1.4537409534749237 2216 20 -2.2906566657049083 2216 21 -5.045515091104676 2216 22 -3.462124929723366 2216 23 -3.2928837476115334 2216 24 -3.5251157214096978 2216 25 -3.133759150034656 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 44020.0 3 2.0 4 285.0 5 1892.0 6 2241.0 7 1949.0 8 1434.0 9 1413.0 10 1301.0 11 1289.0 12 1249.0 13 1286.0 14 1308.0 15 1353.0 16 1369.0 17 1587.0 18 1728.0 19 2250.0 20 2841.0 21 3739.0 22 4504.0 23 5269.0 24 6386.0 25 7320.0 26 8088.0 27 8792.0 28 9782.0 29 12031.0 30 14076.0 31 16750.0 32 21715.0 33 28476.0 34 37845.0 35 52117.0 36 74930.0 37 115862.0 38 151371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.11382878645343 18.636861342010704 12.714094042533505 25.53521582900236 2 14.105880775885845 19.741361112951513 42.25416131880255 23.89859679236009 3 19.00392457784208 24.843949108379675 28.984247758429678 27.16787855534857 4 12.22258837442793 16.687044576806755 38.42124066055227 32.66912638821305 5 12.678579674008402 37.820873928368044 36.48089147465792 13.019654922965632 6 31.36183835865232 37.08131320127441 19.111295808899357 12.445552631173909 7 28.54766097530485 30.936767390858115 22.210046902418583 18.305524731418448 8 24.408931192044705 38.364188814868655 20.627726570589534 16.59915342249711 9 25.97083979123347 15.882201745056722 20.194822085171943 37.952136378537865 10 14.426679720813668 29.618289352557415 34.69893873479454 21.256092191834387 11 34.152216170293975 22.90368404932997 24.383029086706056 18.561070693669997 12 23.013820912980577 24.92858681934312 31.997568258072462 20.06002400960384 13 28.891594190407737 22.197050263715596 25.620043308765023 23.29131223711164 14 20.341367316157232 22.604734201372857 27.54193985286422 29.51195862960569 15 22.99070109610714 31.433896520837116 23.0373341295321 22.53806825352364 16 21.690120034660605 28.50911687795989 28.221920215966968 21.578842871412544 17 21.456022433968624 28.96515316385755 28.045077255286433 21.533747146887393 18 21.245709888418624 28.22793382070027 29.6921893035783 20.83416698730281 19 23.710643616169826 27.057244529816654 27.132201741100566 22.09991011291296 20 23.206584808030314 27.832295479100804 28.087167442876975 20.873952269991904 21 22.69628854396725 27.121255261679583 27.117099785300386 23.065356409052782 22 22.63280049989688 28.563535231340826 27.310407028106887 21.493257240655407 23 22.122761951521326 28.137577359175904 27.54071312808879 22.198947561213984 24 23.22556218554778 27.373522680575803 27.96052580951487 21.440389324361554 25 22.457802546545327 27.666323454042598 27.96105537112366 21.914818628288415 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 186.0 1 186.0 2 252.5 3 319.0 4 319.0 5 319.0 6 476.0 7 633.0 8 633.0 9 633.0 10 936.5 11 1240.0 12 1240.0 13 1240.0 14 2046.0 15 2852.0 16 2852.0 17 2852.0 18 5144.0 19 7436.0 20 7436.0 21 7436.0 22 11879.0 23 16322.0 24 16322.0 25 16322.0 26 23391.5 27 30461.0 28 30461.0 29 30461.0 30 39981.0 31 49501.0 32 49501.0 33 49501.0 34 56719.5 35 63938.0 36 63938.0 37 63938.0 38 69335.0 39 74732.0 40 74732.0 41 74732.0 42 78587.5 43 82443.0 44 82443.0 45 82443.0 46 84630.0 47 86817.0 48 86817.0 49 86817.0 50 82960.0 51 79103.0 52 79103.0 53 79103.0 54 69792.0 55 60481.0 56 60481.0 57 60481.0 58 51375.0 59 42269.0 60 42269.0 61 42269.0 62 34275.0 63 26281.0 64 26281.0 65 26281.0 66 20342.0 67 14403.0 68 14403.0 69 14403.0 70 10469.0 71 6535.0 72 6535.0 73 6535.0 74 4546.5 75 2558.0 76 2558.0 77 2558.0 78 1735.0 79 912.0 80 912.0 81 912.0 82 607.0 83 302.0 84 302.0 85 302.0 86 194.0 87 86.0 88 86.0 89 86.0 90 57.0 91 28.0 92 28.0 93 28.0 94 17.5 95 7.0 96 7.0 97 7.0 98 6.0 99 5.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.21851196429945371 2 0.12879895360467802 3 0.132799876894668 4 0.06986227590982534 5 0.021543433099946142 6 0.021543433099946142 7 0.2937600984842656 8 0.2814495652842964 9 0.019850734784950374 10 0.038162652919904595 11 0.015849811494960377 12 0.01692698314995768 13 0.014926521504962683 14 0.01692698314995768 15 0.014772639839963068 16 0.01861968146495345 17 0.01861968146495345 18 0.015388166499961529 19 0.022466723089943835 20 0.017234746479956913 21 0.016157574824959605 22 0.017850273139955374 23 0.01861968146495345 24 0.016773101484958065 25 0.016465338154958837 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 649850.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 86.96935585562488 #Duplication Level Percentage of deduplicated Percentage of total 1 93.9287727429224 81.68924861761339 2 4.557159403428146 7.926664356950994 3 0.751752136822878 1.9613819730772586 4 0.2698108720188861 0.9386111096930789 5 0.13503648847195704 0.587201820970581 6 0.08144732994359918 0.42500530928132224 7 0.054596677576276884 0.33237665164662283 8 0.03966467405456212 0.2759688922198868 9 0.02427767301909758 0.19002722257299604 >10 0.13372755669261285 2.1624212460584635 >50 0.011877357774230093 0.7198351418716833 >100 0.010458927601052312 1.795932640740211 >500 0.0012408897149787052 0.8240415454595581 >1k 1.7726995928267215E-4 0.1712834718438857 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 1111 0.1709625298145726 No Hit GTCCTACAGTGTGCATTTCTCATTT 923 0.1420327767946449 No Hit CTGTAGGACGTGGAATATGGCAAGA 911 0.14018619681464953 No Hit GTCCTAAAGTGTGTATTTCTCATTT 787 0.12110487035469723 No Hit GTATCAACGCAGAGTACTTTTTTTT 760 0.11695006539970763 No Hit CTTTAGGACGTGAAATATGGCGAGG 706 0.1086404554897284 No Hit CTGTAGGACCTGGAATATGGCGAGA 667 0.10263907055474339 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 1.538816649996153E-4 0.0 9 0.0 0.0 0.0 1.538816649996153E-4 0.0 10 0.0 1.538816649996153E-4 0.0 3.077633299992306E-4 0.0 11 0.0 1.538816649996153E-4 0.0 3.077633299992306E-4 0.0 12 0.0 1.538816649996153E-4 0.0 3.077633299992306E-4 1.538816649996153E-4 13 0.0 3.077633299992306E-4 0.0 3.077633299992306E-4 1.538816649996153E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCACC 50 8.527693E-5 15.235982 4 GGGTAAT 50 0.0014526907 13.354188 1 TAATTGT 50 0.0014714454 13.331485 4 ATCCTAC 70 1.04843486E-4 12.2640505 1 TAGGACC 250 0.0 12.188786 4 GCCCTAG 55 0.0029731689 12.14017 1 GGACTCC 55 0.0030165522 12.116721 6 AGGACGT 515 0.0 12.015889 5 GTCCTAG 80 2.7437023E-5 11.923383 1 TAGACTG 80 2.7985589E-5 11.900352 5 GTCCTAA 330 0.0 11.851119 1 TAAGACA 145 9.276846E-11 11.82102 4 GCCTAGG 65 7.7435724E-4 11.739945 1 CCTAATA 65 7.8193645E-4 11.72633 2 GTAATAC 65 7.8193645E-4 11.72633 3 GGACGTG 515 0.0 11.64617 6 TGTAGGA 690 0.0 11.598868 2 GACGTGG 290 0.0 11.489995 7 CATCTAG 75 1.993509E-4 11.446446 1 AACCATC 75 2.0296485E-4 11.424337 7 >>END_MODULE