FastQCFastQC Report
Thu 26 May 2016
SRR1926855_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926855_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649850
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13490.207586366084481No Hit
CTGTAGGACGTGGAATATGGCAAGA11070.17034700315457413No Hit
GTCCTACAGTGGACATTTCTAAATT10570.16265291990459335No Hit
GTCCTACAGTGTGCATTTCTCATTT9840.15141955835962145No Hit
GGTATCAACGCAGAGTACTTTTTTT9120.14034007847964913No Hit
CTTTAGGACGTGAAATATGGCGAGG8880.13664691851965838No Hit
CTGTAGGACCTGGAATATGGCGAGA8450.13003000692467492No Hit
GTCCTAAAGTGTGTATTTCTCATTT8350.12849119027467878No Hit
TATCAACGCAGAGTACTTTTTTTTT6860.1055628221897361No Hit
GTACATGGGAAGCAGTGGTATCAAC6720.10340847887974147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGAC707.0817114E-613.601763
AATACTC707.0817114E-613.601765
GCCTAAG500.001461803413.3431191
CCTAGGG500.001462655413.3420862
TAGGACC2200.012.9834994
GGTCTAC603.9762227E-412.7077311
GGCGAGG2250.012.65102319
GTCCTAA2750.012.4766821
ACAGGTC853.981211E-612.2808238
AATCCCG1103.8640792E-812.07597519
GATATAC1950.011.7302131
GGACGTG5250.011.4254786
AGGACGT5450.011.35559755
GTAGGAC6300.011.33483
GTCCTAC6850.011.2699951
CTGCTCA1354.782123E-911.2470849
TGTAGGA6950.011.2441022
TAGGACT855.1920946E-511.201454
CTAAGGC600.005772658611.1081053
ATAAGCT600.005772658611.1081054
Produced by FastQC (version 0.11.5)