FastQCFastQC Report
Thu 26 May 2016
SRR1926854_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926854_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620912
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT8750.14092174092302934No Hit
GTCCTACAGTGGACATTTCTAAATT8420.1356069781225037No Hit
CTGTAGGACGTGGAATATGGCAAGA7400.1191795294663334No Hit
GTCCTACAGTGTGCATTTCTCATTT6280.10114154662818563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC1600.014.2870744
TTAGACT551.9144804E-413.8541344
TCCAACG551.9906982E-413.79053618
GACGTGG1450.013.1301057
TGTAGGA4000.012.6335372
GTAGGAC3800.012.5436853
GTATAAC550.002971333712.141031
GTCCTAC5300.012.0592181
CCTTTAT802.7412518E-511.9242261
GCCTAGA751.9919226E-411.4472571
CTCCTAG751.9919226E-411.4472571
CTAAATT2900.011.44257819
TCCTAGA752.0009407E-411.4416942
ATTTCTA3500.011.377192515
TGGCGAG2950.011.24863618
GTCTTGA855.0914292E-511.2228011
CTGTAGG4250.011.22281
GTGTTAA600.005690878311.1292771
CAATTAG600.005690878311.1292771
TGTTAGA600.005711515411.123872
Produced by FastQC (version 0.11.5)