##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1926854_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	620912
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.963299147061097	31.0	28.0	31.0	16.0	34.0
2	28.100089867807355	31.0	28.0	31.0	16.0	34.0
3	27.58905931919499	31.0	27.0	31.0	15.0	34.0
4	31.227038614167547	35.0	32.0	37.0	17.0	37.0
5	31.56205388203159	35.0	32.0	37.0	17.0	37.0
6	30.763939173345015	35.0	31.0	37.0	11.0	37.0
7	30.86346052258613	35.0	32.0	37.0	11.0	37.0
8	30.72219573788234	35.0	32.0	37.0	10.0	37.0
9	32.76097256938181	37.0	32.0	39.0	17.0	39.0
10	32.926817326770944	37.0	33.0	39.0	17.0	39.0
11	33.09829251166027	37.0	33.0	39.0	17.0	39.0
12	32.953172108124825	37.0	33.0	39.0	15.0	39.0
13	33.06573395263741	38.0	33.0	39.0	16.0	39.0
14	34.06251610534182	39.0	34.0	41.0	16.0	41.0
15	33.640638286907	39.0	33.0	41.0	10.0	41.0
16	33.70548483521014	39.0	33.0	40.0	10.0	41.0
17	33.93553031669544	39.0	33.0	41.0	11.0	41.0
18	33.9869707784678	39.0	34.0	41.0	10.0	41.0
19	33.35238810008504	39.0	33.0	40.0	8.0	41.0
20	33.613033731027905	39.0	33.0	41.0	8.0	41.0
21	33.596218143633884	39.0	33.0	41.0	2.0	41.0
22	33.612281611564924	39.0	34.0	41.0	2.0	41.0
23	33.61189669389543	39.0	34.0	41.0	2.0	41.0
24	32.31006487231685	38.0	32.0	40.0	2.0	41.0
25	32.86316096322828	39.0	33.0	40.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	fail
#Tile	Base	Mean
1101	1	-0.5080390709596365
1101	2	-0.6302909483392192
1101	3	-1.280900597846621
1101	4	-1.2864833938243834
1101	5	-0.7083325374301914
1101	6	-2.9329145175300333
1101	7	-1.2532897081726375
1101	8	-0.853008566965773
1101	9	-0.6489397028637143
1101	10	-9.855342063764422
1101	11	-4.58108842581208
1101	12	-2.2041867545425227
1101	13	-1.4813638165847536
1101	14	-1.2757957717059014
1101	15	-2.252141709743537
1101	16	-0.9778025586710015
1101	17	-0.7355000835584207
1101	18	-0.5364118527679764
1101	19	-1.2478825687112618
1101	20	-0.67059551502755
1101	21	-0.7294559518588244
1101	22	-0.256695417143078
1101	23	-0.22527349943904085
1101	24	-0.4820427259384772
1101	25	-0.4810692297474901
1102	1	-0.15153878678487942
1102	2	-0.26724976617222396
1102	3	-0.8340449366707468
1102	4	-0.6157224152875074
1102	5	-0.11505613661937986
1102	6	-0.8202410550618353
1102	7	0.08536001045776231
1102	8	0.526650130611305
1102	9	0.11492814733593804
1102	10	0.3039092272183339
1102	11	0.042149507737370584
1102	12	0.2808959000644009
1102	13	0.056721699283905025
1102	14	0.08910169365039167
1102	15	0.63925962551194
1102	16	0.442416292482406
1102	17	0.11251276650707354
1102	18	-0.23386818376692986
1102	19	0.2763747350678969
1102	20	0.13315071325381211
1102	21	0.11572856659302033
1102	22	0.24378389908122244
1102	23	0.09202408397784012
1102	24	-3.5112322302547625
1102	25	-1.7027487147691645
1103	1	0.013733162096755791
1103	2	-0.27509633396153177
1103	3	-0.1920084438648395
1103	4	-0.3838172970648408
1103	5	-0.3107154615880212
1103	6	0.33130421994759374
1103	7	0.16889125514849823
1103	8	0.35043491872866994
1103	9	0.1834675313948253
1103	10	7.608400904643986E-4
1103	11	-0.04161790514849173
1103	12	-0.3936185393313423
1103	13	-0.22626804547370227
1103	14	-0.3780317666835913
1103	15	-0.12976305988912884
1103	16	-0.017112792956261558
1103	17	-0.25556259524596925
1103	18	-0.45721702467511705
1103	19	-2.422808436261281
1103	20	-0.803179064302256
1103	21	-0.6308798291863198
1103	22	-0.19453101674770323
1103	23	-0.17494357664364912
1103	24	1.3090907659002795
1103	25	0.5748573590912329
1104	1	-0.12951515585035622
1104	2	0.09545078959763842
1104	3	-1.2967912112982098
1104	4	-0.17580342338831656
1104	5	-0.23750052391525145
1104	6	-1.4129324670601768
1104	7	-0.1365121767609594
1104	8	0.11505182792389235
1104	9	0.32505987479439824
1104	10	0.4273894323817089
1104	11	0.26747561177634793
1104	12	0.19310497811506622
1104	13	-0.09304684899949223
1104	14	-0.14882924230034433
1104	15	0.5329918558778317
1104	16	0.048409034613762
1104	17	0.028965126028744237
1104	18	0.012949355129812545
1104	19	-1.107638666272237
1104	20	-0.37091755071543986
1104	21	-0.16127413367701138
1104	22	0.015503928226998198
1104	23	-0.06628817581957236
1104	24	1.5067124213532566
1104	25	0.7257727088017631
1105	1	0.31049322286769865
1105	2	0.21596468430508153
1105	3	0.6016447613313112
1105	4	0.3972659803262495
1105	5	0.6466706812236964
1105	6	0.4742690411090713
1105	7	0.8234096218705176
1105	8	1.1560656528086852
1105	9	0.37517159103514786
1105	10	0.8199672846369808
1105	11	0.7125600971834132
1105	12	0.6482439251894974
1105	13	0.614850092768414
1105	14	0.6433087272569793
1105	15	0.8920897472337899
1105	16	0.43501006798899056
1105	17	0.36705338185893055
1105	18	0.5055760832404133
1105	19	-0.17454971221553706
1105	20	0.5825456527477613
1105	21	0.6663407245830086
1105	22	0.632275328869369
1105	23	0.4120318912101766
1105	24	-4.889712658942791
1105	25	-2.18109784000427
1106	1	-0.05631118006295921
1106	2	-0.5824484550400335
1106	3	-0.4638656151863927
1106	4	-0.5579755211147734
1106	5	-0.19383419741792451
1106	6	0.2548979981330426
1106	7	-2.7840478129941175
1106	8	-0.5243966317613804
1106	9	-0.6031748793284066
1106	10	0.09091419251572574
1106	11	-0.31027055665873604
1106	12	-0.03682037102449698
1106	13	-0.15732370956268227
1106	14	-0.5486423821565438
1106	15	-0.08365593838029639
1106	16	0.07131804164548328
1106	17	-0.1837886612675632
1106	18	-0.27648116411771184
1106	19	0.261551078321169
1106	20	0.25979717768497324
1106	21	-0.24623841589639994
1106	22	-0.6732318067950658
1106	23	-0.8043240007768162
1106	24	-3.3683102954347994
1106	25	-1.7498189761548275
1107	1	0.3114878749098011
1107	2	0.08010270930763852
1107	3	0.5241425188224262
1107	4	0.3678351801763675
1107	5	0.44191202059760926
1107	6	0.42941886341153435
1107	7	-2.0777780562528143
1107	8	-1.4093611051479655
1107	9	-0.5192045373158436
1107	10	0.19371119579956542
1107	11	-0.14851221947019155
1107	12	0.3950448017549917
1107	13	0.04667782266815834
1107	14	0.16335505351647583
1107	15	-1.1396403341777983
1107	16	-0.3117817249962158
1107	17	-0.25232530124901587
1107	18	-0.47432876625601494
1107	19	0.5019658263840014
1107	20	0.5109159448984144
1107	21	0.2063221032434086
1107	22	0.29845715943788775
1107	23	-0.22164434727922355
1107	24	-4.126785021014303
1107	25	-2.0397959358587485
1108	1	0.6466029468931325
1108	2	0.20699301414295945
1108	3	0.6785348042754826
1108	4	0.7825650914817466
1108	5	0.6170858082252622
1108	6	-2.956038226110209
1108	7	-1.2160224497696817
1108	8	0.29520810095942096
1108	9	0.26317052575334543
1108	10	0.44052722338327754
1108	11	0.28962657742576425
1108	12	0.4063084413185294
1108	13	0.46482845423184926
1108	14	0.2053114570415886
1108	15	-0.6639041273958597
1108	16	0.4638292696934414
1108	17	0.2778460180984865
1108	18	0.34222843518288926
1108	19	0.3193948265507274
1108	20	-1.3312830366227217
1108	21	-0.2947997927444632
1108	22	0.06347144059079568
1108	23	0.18890528912621818
1108	24	-0.803984106061538
1108	25	-0.16527174725817417
1109	1	0.3345647315424145
1109	2	0.3896238966541823
1109	3	-0.9492359036202025
1109	4	0.5241872812036164
1109	5	0.4532080517626511
1109	6	0.3793749188054889
1109	7	0.5726345047783603
1109	8	0.9629922310790171
1109	9	0.37765196573004545
1109	10	0.6879994767340847
1109	11	0.27193076807199645
1109	12	0.05968267444735886
1109	13	0.06553945222484714
1109	14	0.4290795564673502
1109	15	-1.1831131025584085
1109	16	0.03189640613793898
1109	17	0.03080428891085063
1109	18	0.20138055583095138
1109	19	-1.6557424030262808
1109	20	-0.29577745563669566
1109	21	0.23457081891640996
1109	22	0.24492787850984143
1109	23	0.3320134203347891
1109	24	-0.1917312617530058
1109	25	0.33772925940861853
1110	1	0.7564224060790679
1110	2	0.8105402101804486
1110	3	1.323449856552216
1110	4	1.0546676145598326
1110	5	1.0219898161956351
1110	6	0.916966948546257
1110	7	1.3161682387882294
1110	8	1.470329646070482
1110	9	1.1980049188991586
1110	10	1.2567802009891622
1110	11	0.8428885846969507
1110	12	0.9081734803147938
1110	13	0.8768251745347868
1110	14	0.5844041231812369
1110	15	1.0871968912629342
1110	16	0.9203654098763394
1110	17	0.6628923212479023
1110	18	0.7681932634764692
1110	19	-0.9205259316010057
1110	20	0.30499394030286453
1110	21	0.3679268281282262
1110	22	0.44128330314167385
1110	23	0.4890563609593528
1110	24	-0.5207280933710834
1110	25	-0.22573407536015822
1111	1	0.46729832628422585
1111	2	0.1252573744500971
1111	3	0.7941178796108801
1111	4	0.8675892186281757
1111	5	0.8681596161485601
1111	6	0.936634632506923
1111	7	0.8557498934827628
1111	8	1.27086950667735
1111	9	0.672905963466178
1111	10	0.6576904769029355
1111	11	0.47065749615089203
1111	12	0.7773583903501304
1111	13	0.5035581260458457
1111	14	0.4816895762918634
1111	15	-0.6473038932252564
1111	16	-0.05289562403034864
1111	17	0.30114630876038007
1111	18	0.4618353315387296
1111	19	0.8672246457470365
1111	20	0.3954916262441941
1111	21	0.628712892548549
1111	22	0.9213911093435385
1111	23	0.36078398749112495
1111	24	0.8386027532435918
1111	25	0.611398296262216
1112	1	0.528897571684471
1112	2	0.5714381389452292
1112	3	-0.7551589451452578
1112	4	0.26364402791584496
1112	5	0.5816565215283731
1112	6	1.5071021737110932
1112	7	1.425836843206632
1112	8	1.9200587953930182
1112	9	0.8988003791605337
1112	10	1.1577167068563057
1112	11	1.050390896962064
1112	12	1.0094029586410969
1112	13	0.8845950012457848
1112	14	1.1677057195423828
1112	15	1.2831414708104987
1112	16	1.0990498670406694
1112	17	0.47670801549715947
1112	18	0.984286628687066
1112	19	1.5677351526434578
1112	20	1.399733678311165
1112	21	1.4029172249139492
1112	22	1.2455789322676907
1112	23	1.2936383927346498
1112	24	2.465130390871124
1112	25	1.3586176640483316
1113	1	-0.45698629582149763
1113	2	-0.0026733085264574186
1113	3	-4.490604342611341
1113	4	-0.8579823555672483
1113	5	0.21181405039932244
1113	6	0.8691743150466031
1113	7	1.1087078809590984
1113	8	1.3958057961778607
1113	9	0.768963193697715
1113	10	0.9852898652821409
1113	11	0.40140418320491733
1113	12	0.45789574290582635
1113	13	0.03177277582013005
1113	14	0.042278261307153286
1113	15	0.4768951747811556
1113	16	0.9279184141231411
1113	17	0.8135971606607768
1113	18	0.48913983132028704
1113	19	1.2949587363251638
1113	20	0.6748075575096664
1113	21	0.7142669918641147
1113	22	0.8908646785877465
1113	23	0.7945523725209753
1113	24	2.1064598961007306
1113	25	1.622147167676225
1114	1	0.6478069372612083
1114	2	0.7380674322142831
1114	3	1.0241373727882674
1114	4	0.7864063940846577
1114	5	0.862642510438306
1114	6	1.6315664063860673
1114	7	1.683530353807889
1114	8	1.2831108644039801
1114	9	0.6420834944495937
1114	10	0.904636843839647
1114	11	0.5450445340821162
1114	12	-0.04300929747289928
1114	13	0.3863397488081546
1114	14	0.6364546745625148
1114	15	-1.7366595453525129
1114	16	0.09242690757900363
1114	17	0.22406778165771613
1114	18	0.5737959159763761
1114	19	1.026022506862624
1114	20	1.1829355162955224
1114	21	1.0381322104661805
1114	22	1.1706265833496516
1114	23	0.36377055687074034
1114	24	1.2040998292669798
1114	25	0.8947557725216697
1115	1	-3.733663925368777
1115	2	-1.1584234166111962
1115	3	-0.2386317961349036
1115	4	0.47420836767405916
1115	5	0.5710336785878241
1115	6	1.51899520319434
1115	7	1.1465027978882567
1115	8	1.1187575903845186
1115	9	0.6977585120844765
1115	10	-0.34496305300579877
1115	11	0.25200405350323507
1115	12	0.04476499125367894
1115	13	0.2853241315115653
1115	14	0.2586909288385044
1115	15	0.5368744842740796
1115	16	0.4858363932473466
1115	17	0.4465877109665044
1115	18	0.27216743925062303
1115	19	-2.059265832879948
1115	20	-0.4673894964091758
1115	21	-0.11184010467099625
1115	22	0.05248996424027297
1115	23	-0.16532888867351403
1115	24	-1.1849597197352253
1115	25	-0.7516137180010034
1116	1	0.9794474420319084
1116	2	0.2233962513381158
1116	3	0.5642009821039551
1116	4	0.8590954266074888
1116	5	1.208851600414711
1116	6	2.1416207820914117
1116	7	1.8817451891418386
1116	8	-1.3509816768907754
1116	9	0.3984700749359007
1116	10	0.3171227369349552
1116	11	0.7892735276185832
1116	12	0.7756528549842727
1116	13	0.7704066411852963
1116	14	0.7747536383462474
1116	15	0.5149563364572742
1116	16	1.0195839815504826
1116	17	0.6754887316936191
1116	18	1.0256785465293632
1116	19	1.5382313916476846
1116	20	1.1296370654901438
1116	21	0.9749845752963822
1116	22	1.1593923574339868
1116	23	1.2829251685461287
1116	24	2.083909001319917
1116	25	1.3251959079479576
1201	1	-0.21311997328037435
1201	2	-0.44188380165295627
1201	3	-0.12491352409702472
1201	4	-0.44104092897769576
1201	5	-0.4981903637764873
1201	6	-0.3985904904594051
1201	7	-0.18802044904107973
1201	8	-0.09742317342738716
1201	9	-0.2256339451956748
1201	10	-0.4175194423228561
1201	11	-0.028954310930529914
1201	12	-0.048477786452941984
1201	13	-0.2615672238195259
1201	14	-0.16464411225823739
1201	15	0.35869660893786914
1201	16	-0.2637694225703697
1201	17	-0.39558072161585756
1201	18	-0.6022876590856328
1201	19	-0.6935689108290433
1201	20	-0.09263406726736889
1201	21	-0.053880675829518054
1201	22	-0.12594297144918443
1201	23	-0.18017759703021596
1201	24	-1.1885128981626423
1201	25	-0.7086288714567957
1202	1	-0.7037266586332898
1202	2	-0.9029000658960733
1202	3	-1.2500998074639469
1202	4	-1.4237419927554562
1202	5	-0.9471995550428538
1202	6	-0.5883086781506748
1202	7	-0.7409236383152766
1202	8	-0.10263524690603987
1202	9	-0.39401866571271427
1202	10	-0.4634062798425731
1202	11	-0.6775357260921311
1202	12	-0.4950521028991588
1202	13	-0.7522408296220462
1202	14	-0.7880788815499855
1202	15	-0.2682862311145371
1202	16	-0.43319269698797314
1202	17	-0.6977407078199533
1202	18	-0.7750325042806168
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2210	23	1.1277299272894439
2210	24	1.6024916421324775
2210	25	1.502779792627031
2211	1	0.7156467245227915
2211	2	0.4266805926584283
2211	3	0.06038213179062524
2211	4	0.6938844005248583
2211	5	0.6195661183626555
2211	6	-1.7270139255078334
2211	7	0.2922116815403655
2211	8	0.6163932095469633
2211	9	0.6021009088776879
2211	10	0.6334286982801274
2211	11	0.8196218223732643
2211	12	0.7007417075827789
2211	13	0.634332579084159
2211	14	0.8250443019957316
2211	15	1.172708946299153
2211	16	0.0835745733004245
2211	17	0.35685671800484187
2211	18	0.28625586083068555
2211	19	0.6827856980957918
2211	20	0.4681928693808928
2211	21	-0.2438148324148912
2211	22	-3.5797646529570777
2211	23	-1.6657790605217215
2211	24	0.7244735334805625
2211	25	8.778853153899036E-4
2212	1	-0.6714188714700846
2212	2	-0.2586316838201803
2212	3	-2.942035191889623
2212	4	-1.6627346693830063
2212	5	-0.5907623175093697
2212	6	-0.17011259810737656
2212	7	0.11309873599194731
2212	8	-0.18994025954553706
2212	9	-0.027966504667027436
2212	10	0.17373397404347202
2212	11	-0.2529890695644639
2212	12	-0.48291613068550276
2212	13	-0.40527465140856833
2212	14	-0.5265577574910978
2212	15	-1.452857139034606
2212	16	-0.47958889897329726
2212	17	-0.37098059793510885
2212	18	-0.396272339590368
2212	19	0.49345535551665165
2212	20	-0.9647856664868755
2212	21	-0.9877183339882762
2212	22	-0.5062784183287619
2212	23	-0.3879063864105916
2212	24	0.8735137972180738
2212	25	0.2877258613893545
2213	1	-0.6694273920516558
2213	2	-0.6636976140820146
2213	3	0.14437829627754795
2213	4	-7.557402618464074
2213	5	-1.9930470607117883
2213	6	-3.743703909159745
2213	7	-1.0791021388821704
2213	8	-2.737440731599918
2213	9	-0.9382230662229176
2213	10	-0.1318976347178591
2213	11	0.03356564153825303
2213	12	0.1784438579851937
2213	13	-0.18119535414018628
2213	14	-0.27336844008173244
2213	15	-2.1040355047823383
2213	16	-0.8464646463927323
2213	17	-0.4329151161249385
2213	18	-0.13804580856066195
2213	19	0.3977768166823097
2213	20	-0.989394451418903
2213	21	-0.1745908950406445
2213	22	-0.5535487638725698
2213	23	-0.31404757787584714
2213	24	-0.4141099203675225
2213	25	-0.1819357936333006
2214	1	0.7394108269084789
2214	2	0.6718383789535274
2214	3	0.593349387776108
2214	4	1.027956073598844
2214	5	0.7898239767521886
2214	6	0.9961927497019261
2214	7	1.2676010011274386
2214	8	-1.5234825483626473
2214	9	-0.5478738677358876
2214	10	-0.13658813663909086
2214	11	0.18544596994843232
2214	12	-0.8828366115152875
2214	13	-0.17305545710608783
2214	14	0.14840618317715126
2214	15	-1.4845487207405483
2214	16	-0.1552319449638162
2214	17	-0.023647247188819165
2214	18	0.14915080977507245
2214	19	1.2171146303830795
2214	20	-0.5946877766470493
2214	21	0.08636799364811765
2214	22	0.36483087409573045
2214	23	-0.16010718102185706
2214	24	-1.5501260331797226
2214	25	-0.4097675434531851
2215	1	0.188985813879345
2215	2	-0.27751052759808204
2215	3	0.21220231565373027
2215	4	0.3676605165784572
2215	5	0.32069348749237037
2215	6	1.104561538619084
2215	7	0.5870943993976034
2215	8	-0.8646043067416933
2215	9	-0.30995555086374793
2215	10	-0.3916004938861022
2215	11	-0.45016323960541627
2215	12	-0.7880834931490242
2215	13	-0.20487468719784374
2215	14	-0.3384584536686859
2215	15	-0.3396012095318639
2215	16	0.020403486286852512
2215	17	-0.40352409237730313
2215	18	-1.4046399868451616
2215	19	0.023354631545259963
2215	20	-3.958412129230812
2215	21	-2.4606812483015155
2215	22	-1.4154897787591452
2215	23	-1.2653671371858692
2215	24	-1.3424516470924743
2215	25	-0.7711094260765918
2216	1	-1.0484350337866282
2216	2	-1.5236204852113389
2216	3	-0.6275578945239104
2216	4	-0.7079693448985012
2216	5	-1.031583035210879
2216	6	-2.304713272775409
2216	7	-10.209289126587088
2216	8	-11.309226079960574
2216	9	-5.985037586942408
2216	10	-3.718489733989262
2216	11	-2.6141923559571936
2216	12	-2.381487551395498
2216	13	-2.059366958762226
2216	14	-2.2882089882374714
2216	15	-3.291352288138441
2216	16	-2.430070136488361
2216	17	-2.8014171510748476
2216	18	-2.496028006306563
2216	19	-2.0589965069743386
2216	20	-2.745323199711251
2216	21	-5.437437405741921
2216	22	-4.015165766377123
2216	23	-3.6889728681763927
2216	24	-4.290723294115246
2216	25	-3.732604979084119
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	40011.0
3	7.0
4	291.0
5	1718.0
6	2252.0
7	1840.0
8	1460.0
9	1368.0
10	1204.0
11	1179.0
12	1191.0
13	1192.0
14	1224.0
15	1259.0
16	1375.0
17	1445.0
18	1673.0
19	2129.0
20	2721.0
21	3422.0
22	4301.0
23	5096.0
24	5909.0
25	6893.0
26	7761.0
27	8411.0
28	9474.0
29	11382.0
30	13276.0
31	16016.0
32	20477.0
33	26785.0
34	35989.0
35	49707.0
36	70009.0
37	109915.0
38	150550.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95930581649848	17.993278971120883	12.354167877226624	25.69324733515402
2	14.57301586021722	19.363011102958367	41.54086807879311	24.5231049580313
3	19.496170723329318	24.147262256668782	28.666115134714097	27.690451885287803
4	12.46329490109366	16.035817085316996	37.620671538116895	33.88021647547245
5	13.006728729273759	37.63094603323931	35.93740686984411	13.42491836764282
6	32.271985540841165	36.420778275178286	18.473282934616446	12.83395324936411
7	28.948461002864267	30.842785299681587	21.635296531380096	18.57345716607405
8	25.179516754627855	37.627034859770966	20.06322866840166	17.13021971719952
9	26.01240987377496	15.88186779553992	19.623354531923532	38.482367798761594
10	14.828664551792139	29.169318315468608	34.12821037470013	21.873806758039127
11	35.03969794446959	22.69282376496626	23.482433518627992	18.785044771936153
12	23.252266415809174	24.594235861882332	31.39896005824705	20.75453766406144
13	29.023939228957733	21.82380779031671	25.233964002564342	23.91828897816121
14	20.833460855110026	21.906093500577473	27.07273895835917	30.18770668595333
15	23.466222093360393	30.52237363487001	22.818047098998097	23.193357172771496
16	22.441333006868643	28.13116148403974	27.314784984342584	22.11272052474903
17	21.970789162963094	28.48757323247374	27.299906410630204	22.24173119393296
18	21.88109577831864	28.00413647133867	28.592874895903638	21.521892854439056
19	23.934885743973062	26.97296034667461	26.745495404789327	22.346658504562992
20	23.48607124332718	27.48589727481548	27.507965502688457	21.52006597916888
21	23.20002448398393	26.747141655954927	26.689636509485933	23.363197350575213
22	22.997522575490574	27.711716703768975	26.989590951118387	22.301169769622074
23	22.64033930467027	27.434805790602113	27.136963815985528	22.78789108874209
24	23.541009590757824	26.97847636781861	27.33607600442006	22.144438037003507
25	23.00337140730885	27.03456296542093	27.400698121322336	22.561367505947885
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	200.0
1	200.0
2	251.0
3	302.0
4	302.0
5	302.0
6	435.5
7	569.0
8	569.0
9	569.0
10	840.5
11	1112.0
12	1112.0
13	1112.0
14	1901.0
15	2690.0
16	2690.0
17	2690.0
18	4496.5
19	6303.0
20	6303.0
21	6303.0
22	10001.5
23	13700.0
24	13700.0
25	13700.0
26	19647.0
27	25594.0
28	25594.0
29	25594.0
30	33950.5
31	42307.0
32	42307.0
33	42307.0
34	48940.5
35	55574.0
36	55574.0
37	55574.0
38	61461.5
39	67349.0
40	67349.0
41	67349.0
42	71720.5
43	76092.0
44	76092.0
45	76092.0
46	79574.5
47	83057.0
48	83057.0
49	83057.0
50	80921.5
51	78786.0
52	78786.0
53	78786.0
54	70894.5
55	63003.0
56	63003.0
57	63003.0
58	54511.5
59	46020.0
60	46020.0
61	46020.0
62	37749.5
63	29479.0
64	29479.0
65	29479.0
66	22992.5
67	16506.0
68	16506.0
69	16506.0
70	12040.0
71	7574.0
72	7574.0
73	7574.0
74	5308.0
75	3042.0
76	3042.0
77	3042.0
78	2095.5
79	1149.0
80	1149.0
81	1149.0
82	761.0
83	373.0
84	373.0
85	373.0
86	236.0
87	99.0
88	99.0
89	99.0
90	61.0
91	23.0
92	23.0
93	23.0
94	15.0
95	7.0
96	7.0
97	7.0
98	4.5
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.21967686242172801
2	0.1299701084855825
3	0.13222485634035097
4	0.06860875615224057
5	0.022708531965882444
6	0.020775890947509472
7	0.3068067616667096
8	0.30422990697554564
9	0.018682196510938748
10	0.03688123276728425
11	0.015944288401577033
12	0.017393769165356766
13	0.014494807637797303
14	0.016588502074368027
15	0.0148169144741928
16	0.01932641018372974
17	0.018521143092741
18	0.014977967892390549
19	0.025285386657046407
20	0.017715876001752263
21	0.015783234983379288
22	0.01707166232896127
23	0.018199036256345506
24	0.01707166232896127
25	0.016588502074368027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	620912.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.8432611524302
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.38731234131185	81.10058753980114
2	4.97265022391045	8.636823240294914
3	0.832629784974776	2.169248575795688
4	0.29104257475171863	1.0110034530255674
5	0.152383630111911	0.6616745692582007
6	0.08864854388097373	0.46191171882228416
7	0.05944373472402694	0.36136014449601184
8	0.03499724862535133	0.24314201615903314
9	0.025426927694309022	0.19873415898547545
>10	0.13393083125160826	2.098081216862758
>50	0.009653321374877599	0.5694151393285103
>100	0.010581370799365808	1.7382111402480707
>500	0.0012994665887870545	0.7498070869223941
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	875	0.14092174092302934	No Hit
GTCCTACAGTGGACATTTCTAAATT	842	0.1356069781225037	No Hit
CTGTAGGACGTGGAATATGGCAAGA	740	0.1191795294663334	No Hit
GTCCTACAGTGTGCATTTCTCATTT	628	0.10114154662818563	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.221068363954957E-4	0.0
11	0.0	0.0	0.0	3.221068363954957E-4	0.0
12	0.0	0.0	0.0	4.8316025459324346E-4	0.0
13	0.0	0.0	0.0	4.8316025459324346E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	160	0.0	14.287074	4
TTAGACT	55	1.9144804E-4	13.854134	4
TCCAACG	55	1.9906982E-4	13.790536	18
GACGTGG	145	0.0	13.130105	7
TGTAGGA	400	0.0	12.633537	2
GTAGGAC	380	0.0	12.543685	3
GTATAAC	55	0.0029713337	12.14103	1
GTCCTAC	530	0.0	12.059218	1
CCTTTAT	80	2.7412518E-5	11.924226	1
GCCTAGA	75	1.9919226E-4	11.447257	1
CTCCTAG	75	1.9919226E-4	11.447257	1
CTAAATT	290	0.0	11.442578	19
TCCTAGA	75	2.0009407E-4	11.441694	2
ATTTCTA	350	0.0	11.3771925	15
TGGCGAG	295	0.0	11.248636	18
GTCTTGA	85	5.0914292E-5	11.222801	1
CTGTAGG	425	0.0	11.2228	1
GTGTTAA	60	0.0056908783	11.129277	1
CAATTAG	60	0.0056908783	11.129277	1
TGTTAGA	60	0.0057115154	11.12387	2
>>END_MODULE