FastQCFastQC Report
Thu 26 May 2016
SRR1926854_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926854_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences620912
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT14430.23240008245935012No Hit
GGTATCAACGCAGAGTACTTTTTTT9610.15477233488803566No Hit
CTGTAGGACGTGGAATATGGCAAGA8860.14269332852320457No Hit
GTCCTACAGTGGACATTTCTAAATT8140.13109748241296673No Hit
TATCAACGCAGAGTACTTTTTTTTT7380.1188574226299379No Hit
GTCCTACAGTGTGCATTTCTCATTT6630.10677841626510681No Hit
GTCCTAAAGTGTGTATTTCTCATTT6410.10323524106475636No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAG500.00147143613.3313423
CCTAGAC655.3232772E-513.1848443
TGGACTG1102.6448106E-912.9829765
TAGGACC2600.012.450344
CTTATAC701.06518986E-412.243073
AGGACCT4900.012.0467895
GGACTGC951.0093245E-612.0263356
GGACCTG4450.011.9812936
GGCGAGA4350.011.77900819
GTGTAAG1302.4610927E-911.7331371
TAACTGA657.865464E-411.7179674
GATATAC1800.011.6516581
TGGCGAG6200.011.63124218
TGTAGGA5550.011.5085162
TTACACA1001.8731625E-611.4250184
TTTACAC855.184814E-511.2028083
GTCTCTA600.005718540411.1220361
ATTACAC600.005766954711.1094513
ATACCCT600.005773899611.1076564
TCCAACG600.00592140411.07008518
Produced by FastQC (version 0.11.5)