##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1926854_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 620912 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.096458113226994 31.0 30.0 33.0 26.0 34.0 2 30.366124346123122 31.0 30.0 34.0 26.0 34.0 3 30.42055073826887 31.0 30.0 34.0 26.0 34.0 4 34.03479398046744 37.0 35.0 37.0 30.0 37.0 5 34.04240053340892 37.0 35.0 37.0 30.0 37.0 6 34.0188110392455 37.0 35.0 37.0 30.0 37.0 7 34.0235733888216 37.0 35.0 37.0 30.0 37.0 8 34.05156608343855 37.0 35.0 37.0 30.0 37.0 9 35.631235988352614 39.0 35.0 39.0 30.0 39.0 10 35.524560646275155 39.0 35.0 39.0 30.0 39.0 11 35.53904418017368 39.0 35.0 39.0 30.0 39.0 12 35.42878217847296 39.0 35.0 39.0 30.0 39.0 13 35.44758355451336 39.0 35.0 39.0 30.0 39.0 14 36.592116113072386 40.0 37.0 41.0 30.0 41.0 15 36.574856018244134 40.0 37.0 41.0 30.0 41.0 16 36.55967351251063 40.0 37.0 41.0 30.0 41.0 17 36.539516066688996 40.0 37.0 41.0 30.0 41.0 18 36.5214651995774 40.0 37.0 41.0 30.0 41.0 19 36.589052877058265 40.0 37.0 41.0 30.0 41.0 20 36.54427358466256 40.0 37.0 41.0 30.0 41.0 21 36.50534536294998 40.0 37.0 41.0 30.0 41.0 22 36.413548135645634 40.0 37.0 41.0 30.0 41.0 23 36.35070670239905 40.0 37.0 41.0 30.0 41.0 24 36.283824438889894 40.0 37.0 41.0 30.0 41.0 25 36.16387507408457 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09877948902705924 1101 2 -0.17498158143261833 1101 3 -0.01707535316469233 1101 4 -0.0190291149636721 1101 5 0.025739331818172673 1101 6 0.19491309354518194 1101 7 0.11502543825477574 1101 8 0.24117575099757005 1101 9 0.2945071004476958 1101 10 0.19383055315158515 1101 11 0.22864595345405547 1101 12 0.24957243257978945 1101 13 0.4460895831190328 1101 14 0.20156207855455222 1101 15 0.2619335706873258 1101 16 0.21942139717945963 1101 17 0.3795069149692907 1101 18 0.3508767368685639 1101 19 0.405741167689051 1101 20 0.36951999072078934 1101 21 0.29248691177723174 1101 22 0.21292134613954516 1101 23 0.18902914433824236 1101 24 0.27413829086029295 1101 25 0.279161810916726 1102 1 -0.6038068210285523 1102 2 -0.5363567433390521 1102 3 -0.2579689547199706 1102 4 -0.40205522245246783 1102 5 -0.5735401083209624 1102 6 -0.40018909960096494 1102 7 -0.26050601732980994 1102 8 -0.39071670654087143 1102 9 -0.5249894833570323 1102 10 -0.40733931993328554 1102 11 -0.45435893240038183 1102 12 -0.16418601643584196 1102 13 -0.10249820248879615 1102 14 -0.3407567874783908 1102 15 -0.42256414737716597 1102 16 -0.30965346152591877 1102 17 -0.39153360371672363 1102 18 -0.2688084023770685 1102 19 -0.3150101757379744 1102 20 -0.16909850527515147 1102 21 -0.20679106480091747 1102 22 -0.1926411613307124 1102 23 -0.20816667280444534 1102 24 -0.2564440356650479 1102 25 -0.21631822489172947 1103 1 -0.5183958702949276 1103 2 -0.46670278999190984 1103 3 -0.2719173375100574 1103 4 -0.3665299831815574 1103 5 -0.41804022098283156 1103 6 -0.4327550067940962 1103 7 -0.5127228052736612 1103 8 -0.3379656483025144 1103 9 -0.29133961234582983 1103 10 -0.18578101952493142 1103 11 -0.37616798077510083 1103 12 -0.3922943694398171 1103 13 -0.23022543304318077 1103 14 -0.32568660576142605 1103 15 -0.43549917581977127 1103 16 -0.31871340366470946 1103 17 -0.34317093612466465 1103 18 -0.24922210639807219 1103 19 -0.5288219183580196 1103 20 -0.6527998874615619 1103 21 -0.7318098317378485 1103 22 -0.5004598950113106 1103 23 -0.6602215168430661 1103 24 -0.5897785739381476 1103 25 -0.5185701368299149 1104 1 -0.08386554118628453 1104 2 0.011508757496745403 1104 3 0.08645120158137232 1104 4 0.12757631212956255 1104 5 0.1584866657850128 1104 6 0.16556033248510005 1104 7 0.20239741587065652 1104 8 -0.11435692770584183 1104 9 -0.07102980167457673 1104 10 0.041048375979165996 1104 11 0.01177339511808384 1104 12 0.1304720207964536 1104 13 0.26648869620107263 1104 14 0.12282171322882363 1104 15 0.19969093527395643 1104 16 0.18626755914757354 1104 17 0.17754830447409375 1104 18 0.17254287560787418 1104 19 0.3478275366046901 1104 20 0.2799571145725466 1104 21 0.23473165890002434 1104 22 0.2566073349474891 1104 23 0.19581301087820435 1104 24 0.23570517925644907 1104 25 0.15074348127888726 1105 1 -0.08510140209956418 1105 2 -0.1846160854022898 1105 3 -0.09437549860016503 1105 4 0.004967737908081915 1105 5 -0.30757254382258026 1105 6 -0.3532498895509306 1105 7 -0.6004932418587146 1105 8 -0.6927607819161494 1105 9 -0.47075051107270127 1105 10 -0.37590456355442114 1105 11 -0.46641400887577333 1105 12 -0.21755199819530446 1105 13 -0.12236760878426622 1105 14 -0.08109533746242192 1105 15 -0.2851994431648137 1105 16 -0.0791981579310459 1105 17 -0.2683461236783984 1105 18 -0.2729675279670474 1105 19 -0.06377698790306141 1105 20 -0.175014258140763 1105 21 -0.1627547256831292 1105 22 -0.12215959704525403 1105 23 -0.23807192139382494 1105 24 -0.05940723521080571 1105 25 -0.1687579849967733 1106 1 0.30136109081107065 1106 2 0.230026784141792 1106 3 0.4000780125171204 1106 4 0.26982068419093963 1106 5 0.2874469591694222 1106 6 0.5732626050958771 1106 7 0.4624545456802096 1106 8 0.3195185796357549 1106 9 0.20871521111672564 1106 10 0.22712100446298678 1106 11 0.27108960814796745 1106 12 0.003397803302426894 1106 13 0.034127408403833215 1106 14 0.32425438532887796 1106 15 0.3362642650658003 1106 16 0.23140290460585078 1106 17 0.17765431615168836 1106 18 0.41731801435502547 1106 19 0.24682986187181655 1106 20 0.3851568126140066 1106 21 0.5093438425541876 1106 22 0.3335240493776581 1106 23 0.2540180817312887 1106 24 0.4016052999466666 1106 25 0.6095375576725317 1107 1 0.48997552447104553 1107 2 0.3449986572485244 1107 3 0.39780580966248635 1107 4 0.4972353401835221 1107 5 0.3174084374311832 1107 6 0.49214416751666334 1107 7 0.445827340398786 1107 8 0.3759530966590887 1107 9 0.43864531729304446 1107 10 0.4133427482735357 1107 11 0.5447849101781728 1107 12 0.3722471411354604 1107 13 0.4198437888381079 1107 14 0.4915676276452743 1107 15 0.36023208367149095 1107 16 0.45583607581218644 1107 17 0.5968228876874306 1107 18 0.2997045207209439 1107 19 0.2927024434648118 1107 20 0.10399131690664376 1107 21 0.5315930164794409 1107 22 0.48331893731906916 1107 23 0.34737958931912516 1107 24 0.2438969171208356 1107 25 0.22234918989620667 1108 1 0.3917586797939734 1108 2 0.3655120813143107 1108 3 0.42724972194645616 1108 4 0.5252080921142834 1108 5 0.5828798680419922 1108 6 0.6462607466220476 1108 7 0.5567190488300611 1108 8 0.45595588641501905 1108 9 0.3922808814503469 1108 10 0.5330537905851997 1108 11 0.6176999762768602 1108 12 0.6232815848134479 1108 13 0.3895302573231305 1108 14 0.4305395372005165 1108 15 0.5762540006915131 1108 16 0.5158615977199403 1108 17 0.4405889274218353 1108 18 0.4073880098338165 1108 19 0.2761644215979189 1108 20 0.36517349777654573 1108 21 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24 -0.9620924693608757 2216 25 -0.7854628442717058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12726.0 3 13.0 4 190.0 5 1395.0 6 1860.0 7 1364.0 8 1119.0 9 968.0 10 886.0 11 829.0 12 692.0 13 674.0 14 739.0 15 699.0 16 707.0 17 776.0 18 832.0 19 992.0 20 1353.0 21 1758.0 22 2191.0 23 2713.0 24 3201.0 25 3756.0 26 4572.0 27 5066.0 28 5902.0 29 7480.0 30 9508.0 31 12294.0 32 16068.0 33 21447.0 34 29168.0 35 41189.0 36 60096.0 37 105852.0 38 259837.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.42925581747848 18.779760609007074 10.718414853013028 28.072568720501422 2 14.602969866017446 20.185415087951245 39.73848411073311 25.473130935298194 3 18.990296329228926 23.891584352991966 28.89274415427934 28.225375163499766 4 12.289342128997346 16.825089545700518 37.04035354446363 33.8452147808385 5 13.24507151140317 38.182096379332556 35.010952196881846 13.561879912382423 6 32.83409132783181 37.12217449065679 17.040653717728667 13.003080463782732 7 28.906401020658013 31.56017629547534 20.701347990257354 18.83207469360929 8 25.584646731470816 37.90350248451707 19.007320667799533 17.504530116212578 9 26.146132151296047 15.830419312908614 19.223514896536837 38.7999336392585 10 14.883216780964222 29.629856303974588 33.35963948757759 22.127287427483598 11 35.046312775110444 22.804632727050173 23.144630182303704 19.00442431553568 12 23.240689889533282 24.839630800549514 31.10091462383498 20.818764686082226 13 29.307468071056995 21.54321882398415 25.247298320207438 23.90201478475141 14 21.028583760661736 21.977671554101065 26.83858582214549 30.155158863091707 15 23.289767190909895 30.532054534180496 22.908060138992905 23.2701181359167 16 22.634754263111443 28.345745263388455 26.760325201157016 22.259175272343086 17 21.88026947501333 28.74614880393486 27.0889064616346 22.28467525941721 18 21.802711830558103 27.975274959776712 28.61514328186578 21.606869927799405 19 23.553494065162422 26.958013238633615 26.929667745728043 22.558824950475913 20 23.40371390378638 27.53362000934113 27.544088515243754 21.51857757162874 21 23.15444943954152 26.70291915191091 26.871564081046213 23.271067327501367 22 23.0976370242482 27.682666786919885 26.859200659674805 22.36049552915711 23 22.64507691911253 27.31433761950164 27.236226711675727 22.804358749710104 24 23.084306768602907 26.886461988916288 27.70686942250983 22.32236181997098 25 22.890363147612167 27.179296382375252 27.41122637817152 22.519114091841058 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 180.0 1 180.0 2 194.0 3 208.0 4 208.0 5 208.0 6 341.5 7 475.0 8 475.0 9 475.0 10 790.5 11 1106.0 12 1106.0 13 1106.0 14 1774.0 15 2442.0 16 2442.0 17 2442.0 18 4248.0 19 6054.0 20 6054.0 21 6054.0 22 9715.0 23 13376.0 24 13376.0 25 13376.0 26 19091.5 27 24807.0 28 24807.0 29 24807.0 30 33369.5 31 41932.0 32 41932.0 33 41932.0 34 48272.0 35 54612.0 36 54612.0 37 54612.0 38 60408.5 39 66205.0 40 66205.0 41 66205.0 42 71065.0 43 75925.0 44 75925.0 45 75925.0 46 79354.5 47 82784.0 48 82784.0 49 82784.0 50 81313.0 51 79842.0 52 79842.0 53 79842.0 54 71823.0 55 63804.0 56 63804.0 57 63804.0 58 55658.5 59 47513.0 60 47513.0 61 47513.0 62 38884.0 63 30255.0 64 30255.0 65 30255.0 66 23612.0 67 16969.0 68 16969.0 69 16969.0 70 12343.5 71 7718.0 72 7718.0 73 7718.0 74 5404.0 75 3090.0 76 3090.0 77 3090.0 78 2113.5 79 1137.0 80 1137.0 81 1137.0 82 743.5 83 350.0 84 350.0 85 350.0 86 228.0 87 106.0 88 106.0 89 106.0 90 63.5 91 21.0 92 21.0 93 21.0 94 11.0 95 1.0 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.12159533073929961 2 0.10983843121086401 3 0.018682196510938748 4 0.0 5 0.00515370938232793 6 0.3501301311619038 7 0.021903264874893705 8 0.010790579019249106 9 0.010146365346458113 10 0.0028989615275594608 11 0.004026335454943696 12 1.610534181977478E-4 13 3.221068363954956E-4 14 0.0 15 0.002737908109361713 16 6.442136727909912E-4 17 1.610534181977478E-4 18 4.8316025459324346E-4 19 3.221068363954956E-4 20 3.221068363954956E-4 21 0.013045326874017575 22 0.0 23 0.0 24 1.610534181977478E-4 25 0.005636869636921174 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 620912.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 83.35854610040711 #Duplication Level Percentage of deduplicated Percentage of total 1 91.20987306452456 76.03122408661453 2 6.543405934082262 10.908976104197475 3 1.1758916581459358 2.940618569839265 4 0.42434071159243253 1.414896990782294 5 0.1925016623592408 0.802332934808889 6 0.12240668744551512 0.6122186099055059 7 0.06056707068918132 0.3534148067947517 8 0.04132489980000146 0.2755826852058501 9 0.032871678196455714 0.24661217721033005 >10 0.17146903391656756 2.548586457692789 >50 0.010835683600943816 0.6108194184702227 >100 0.01238350135242914 1.9058297213164488 >500 0.0019349220859246287 1.1161426599496889 >1k 1.9349220859246285E-4 0.2327447772120522 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 1443 0.23240008245935012 No Hit GGTATCAACGCAGAGTACTTTTTTT 961 0.15477233488803566 No Hit CTGTAGGACGTGGAATATGGCAAGA 886 0.14269332852320457 No Hit GTCCTACAGTGGACATTTCTAAATT 814 0.13109748241296673 No Hit TATCAACGCAGAGTACTTTTTTTTT 738 0.1188574226299379 No Hit GTCCTACAGTGTGCATTTCTCATTT 663 0.10677841626510681 No Hit GTCCTAAAGTGTGTATTTCTCATTT 641 0.10323524106475636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.6105341819774784E-4 0.0 0.0 0.0 6 0.0 1.6105341819774784E-4 0.0 0.0 0.0 7 0.0 1.6105341819774784E-4 0.0 0.0 0.0 8 0.0 1.6105341819774784E-4 0.0 0.0 0.0 9 0.0 1.6105341819774784E-4 0.0 0.0 0.0 10 0.0 1.6105341819774784E-4 0.0 3.221068363954957E-4 0.0 11 0.0 1.6105341819774784E-4 0.0 3.221068363954957E-4 0.0 12 0.0 1.6105341819774784E-4 0.0 4.8316025459324346E-4 0.0 13 0.0 1.6105341819774784E-4 0.0 4.8316025459324346E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGAG 50 0.001471436 13.331342 3 CCTAGAC 65 5.3232772E-5 13.184844 3 TGGACTG 110 2.6448106E-9 12.982976 5 TAGGACC 260 0.0 12.45034 4 CTTATAC 70 1.06518986E-4 12.24307 3 AGGACCT 490 0.0 12.046789 5 GGACTGC 95 1.0093245E-6 12.026335 6 GGACCTG 445 0.0 11.981293 6 GGCGAGA 435 0.0 11.779008 19 GTGTAAG 130 2.4610927E-9 11.733137 1 TAACTGA 65 7.865464E-4 11.717967 4 GATATAC 180 0.0 11.651658 1 TGGCGAG 620 0.0 11.631242 18 TGTAGGA 555 0.0 11.508516 2 TTACACA 100 1.8731625E-6 11.425018 4 TTTACAC 85 5.184814E-5 11.202808 3 GTCTCTA 60 0.0057185404 11.122036 1 ATTACAC 60 0.0057669547 11.109451 3 ATACCCT 60 0.0057738996 11.107656 4 TCCAACG 60 0.005921404 11.070085 18 >>END_MODULE