FastQCFastQC Report
Thu 26 May 2016
SRR1926853_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926853_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682476
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17610.2580310516413764No Hit
GTCCTAAAGTGTGTATTTCTCATTT13650.20000703321435478No Hit
CTGTAGGACGTGGAATATGGCAAGA13220.19370644535485496No Hit
GTCCTACAGTGTGCATTTCTCATTT11960.1752442576735299No Hit
CTTTAGGACGTGAAATATGGCGAGG10890.15956605067430943No Hit
CTGTAGGACCTGGAATATGGCGAGA8210.12029727052672914No Hit
GTATCAACGCAGAGTACTTTTTTTT7140.10461906352750866No Hit
GTACATGGGAAGCAGTGGTATCAAC6970.10212813344352037No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG504.3831333E-617.1690581
GACTGCG508.5952175E-515.2221147
TCCAACG653.4201967E-614.59266318
CTAGGAC852.5879854E-713.4550173
AAAGTTC500.001515709613.27932317
TAGGACC2250.013.1137164
CCAACGA604.140787E-412.64697419
GGACGTG6150.011.7569186
TGTAGGA7250.011.70054152
AGGACGT6150.011.6030745
GGGCTAC751.9944526E-411.4460381
TAGACTG1001.8891114E-611.4174255
GTAGAAC3600.011.3838193
GTAGGAC6950.011.24478153
TAGGACG6650.011.1639934
TGTAGAA4200.011.1198972
TGCGGGA600.005727725611.1198962
CCCAAAC600.00573086811.1190773
ATGTCCA1803.6379788E-1211.0661039
CAATCAC600.00593830311.0661029
Produced by FastQC (version 0.11.5)