FastQCFastQC Report
Thu 26 May 2016
SRR1926853_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1926853_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682476
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT17690.2592032540338415No Hit
CTGTAGGACGTGGAATATGGCAAGA15630.2290190424278656No Hit
GTCCTAAAGTGTGTATTTCTCATTT13170.19297381885956427No Hit
CTTTAGGACGTGAAATATGGCGAGG12980.1901898381774597No Hit
GTATCAACGCAGAGTACTTTTTTTT12980.1901898381774597No Hit
GTCCTACAGTGTGCATTTCTCATTT12410.18183789613114598No Hit
CTGTAGGACCTGGAATATGGCGAGA9570.14022471119863555No Hit
GGTATCAACGCAGAGTACTTTTTTT8220.12044379582578728No Hit
GTACATGGGAAGCAGTGGTATCAAC8080.11839244163897339No Hit
GATATACACTGTTCTACAAATCCCG7560.11077312608795034No Hit
CTGAAGGACCTGGAATATGGCGAGA7150.10476558882656681No Hit
CCCATGTACTCTGCGTTGATACCAC6900.10110245635011342No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCTA250.00608009818.97208611
CCAACGA504.6060886E-617.07988419
GTTTAAG508.434114E-515.255911
GTATTAC400.005148109514.3024161
TGTCGAG400.005316442314.23323518
AGACTGT1101.7826096E-1013.8526876
TCCAACG852.7303577E-713.395987518
GGACGAG500.001470143413.333216
TAGGACT1153.601599E-1013.2503964
TAGGACC3900.012.9424944
GTGTAGA2100.012.7132581
TGTAGAA4050.012.7123222
CTTATAG751.4361551E-512.7001643
GGCGAGG3650.012.47845419
TCCTAGG1001.3719364E-712.3945142
AATGCCG1155.3951226E-912.37672718
ATGCCGG1155.3951226E-912.37672719
AATCCCG1850.012.30982519
GTCTTAC701.05245665E-412.2592131
CTGTACT1353.5470293E-1011.9928354
Produced by FastQC (version 0.11.5)